Simple Characterization Scheme for Optical Coherence Tomography Systems With Application to a Commercial and a Near-Isometric Resolution Fibre-Based System

Dey, Rajib; Zhou, Yi; Neuhaus, Kai; Alexandrov, Sergey; Nolan, Andrew; Chang, Ting-Chiao; Leahy, Martin J.

doi:10.1109/jphot.2021.3135058

Cited by 1 publication

(1 citation statement)

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“…In this study, for spectral interference data collection, a custom built fiber-based SDOCT system is used with supercontinuum laser light and a broadband spectrometer. The SDOCT system has an axial and lateral resolution of 3.8 µm in air and 5.5 µm, respectively [ 53 ]. This SDOCT system operates within a wavelength range of 1100 nm to 1500 nm (400 nm bandwidth) with a central wavelength of 1300 nm.…”

Section: Methodsmentioning

confidence: 99%

Skin cancer margin detection using nanosensitive optical coherence tomography and a comparative study with confocal microscopy

Dey¹,

Alexandrov²,

Owens

et al. 2022

Biomed. Opt. Express

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Excision biopsy and histology represent the gold standard for morphological investigation of the skin, in particular for cancer diagnostics. Nevertheless, a biopsy may alter the original morphology, usually requires several weeks for results, is non-repeatable on the same site and always requires an iatrogenic trauma. Hence, diagnosis and clinical management of diseases may be substantially improved by new non-invasive imaging techniques. Optical Coherence Tomography (OCT) is a non-invasive depth-resolved optical imaging modality based on low coherence interferometry that enables high-resolution, cross-sectional imaging in biological tissues and it can be used to obtain both structural and functional information. Beyond the resolution limit, it is not possible to detect structural and functional information using conventional OCT. In this paper, we present a recently developed technique, nanosensitive OCT (nsOCT), improved using broadband supercontinuum laser, and demonstrate nanoscale sensitivity to structural changes within ex vivo human skin tissue. The extended spectral bandwidth permitted access to a wider distribution of spatial frequencies and improved the dynamic range of the nsOCT. Firstly, we demonstrate numerical and experimental detection of a few nanometers structural difference using the nsOCT method from single B-scan images of phantoms with sub-micron periodic structures, acting like Bragg gratings, along the depth. Secondly, our study shows that nsOCT can distinguish nanoscale structural changes at the skin cancer margin from the healthy region in en face images at clinically relevant depths. Finally, we compare the nsOCT en face image with a high-resolution confocal microscopy image to confirm the structural differences between the healthy and lesional/cancerous regions, allowing the detection of the skin cancer margin.

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Section: Methodsmentioning

confidence: 99%