Simple characterisation of a deformable mirror inside a high numerical aperture microscope using phase diversity

Débarre, Delphine; Vieille, Thibault; Beaurepaire, Emmanuel

doi:10.1111/j.1365-2818.2011.03518.x

Cited by 14 publications

(16 citation statements)

References 13 publications

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“…The algorithm is presented in more details in 10 . Briefly, the guess for Π tot ( r) at iteration i was used to calculate three 2D amplitude PSFs corresponding to positions z 0 , 0 and −z 0 .…”

Section: Electric Field Retrievalmentioning

confidence: 99%

“…One of the benefits of our scheme is that it can be used to characterise the properties of an adaptive microscope for several objectives with different magnifications and numerical apertures, without modification of the measuring scheme 10 . Here we illustrate this property on the example of two different objectives: a 25x, 1.05NA and a 60x, 1.2NA objective (Olympus).…”

Section: Adaptive Microscope Characterizationmentioning

confidence: 99%

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Calibration of an adaptive microscope using phase diversity

et al. 2012

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Accurate control over the phase and amplitude modulation in an adaptive microscope is essential to the quality of aberration correction that can be achieved. In this paper we present a robust and compact method for characterising such amplitude and phase modulation in the pupil plane of the focussing objective. This method, based on phase diversity, permits calibrating the microscope as a whole and thus avoids errors in the alignment of the wavefront shaping device after calibration and the resulting imprecision in the induced modulation: by acquiring three 2D images of the point spread function at different distances from the focal plane, we show that the electric field distribution at the pupil plane can be retrieved using an iterative algorithm. We have applied this technique to the characterisation of the phase modulation induced by a deformable mirror when conjugated with the entrance pupil of different objectives, which permits accurate evaluation of the performance of the mirror for subsequent aberration correction.

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Section: Electric Field Retrievalmentioning

confidence: 99%

Section: Adaptive Microscope Characterizationmentioning

confidence: 99%

Calibration of an adaptive microscope using phase diversity

et al. 2012

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“…A range of such methods have been developed specifically for use in microscopes, using interferometry, 23 image based measurements 24,25 and phase diversity. 26,27 Spatial light modulators have been used less frequently in microscopes for aberration correction, due to their polarisation dependent operation and sensitivity to wavelength when used in the common modulo 2p mode. They do, however, have many additional uses in microscopy.…”

Section: Adaptive Correction Elementsmentioning

confidence: 99%

Adaptive optical microscopy: the ongoing quest for a perfect image

2014

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Adaptive optics is becoming a valuable tool for high resolution microscopy, providing correction for aberrations introduced by the refractive index structure of specimens. This is proving particularly promising for applications that require images from deep within biological tissue specimens. We review recent developments in adaptive microscopy, including methods and applications. A range of advances in different microscope modalities is covered and prospects for the future are discussed. INTRODUCTIONOptical microscopes have for a long time played a vital role in biomedical research. Their ability to provide multidimensional structural and functional information about a specimen in a non-invasive manner has permitted numerous scientific advances. These microscopes rely upon high-quality optical systems to operate at the optimum resolution, which is determined by the diffraction limit of light for conventional microscopes. However, even with perfect optical components, the performance of the microscope is affected by the optical properties of the specimen. Spatial variations in refractive index introduce aberrations as the light passes through the specimen-a problem that is exacerbated when imaging deeps into tissue. These aberrations detrimentally affect the resolution and contrast of microscope images, ultimately limiting the depth at which imaging is practical. Adaptive optics (AO) was introduced into microscopy in order to overcome this limitation. Dynamic correction elements, such as deformable mirrors (DMs) or spatial light modulators (SLMs) have been employed to compensate specimen-induced aberrations (Figure 1). New wavefront sensing and control schemes have been developed to adapt the correction elements.The first demonstrations of adaptive optical microscopy took place in the early 2000s. In the few years that followed, there were several innovative advances that set the scene for further research. The research in this period is well covered by two review articles 1,2 and a recent compiled volume, 3 so this will not be reproduced here. This present article provides a review of advances in aberration correction in adaptive microscopy that have taken place in the last half-decade. During this period, a wide range of AO techniques have emerged, which have considerably enhanced the available toolkit. Furthermore, the benefit of correction of specimen-induced aberrations has been shown in different areas, particularly (although not exclusively) in biomedical applications. We outline these technological

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“…Using a phase retrieval algorithm [52,53], the phase and intensity distribution in the pupil can be recovered from defocused PSFs [52] and then used as input to a calibrated DM. However, the images need to be of point-like objects otherwise, for extended object images, the knowledge of the object function is also required, which is rarely the case 1 .…”

Section: Phase Retrieval Techniquementioning

confidence: 99%

“…The algorithm suggested in [52,53] where phase and intensity in the pupil can be recovered is in fact, an extension of the Gerchberg-Saxton algorithm [54], where phase only is recovered from a known intensity distribution in the pupil. From the starting phase and uniform amplitude, the PSF is computed and then compared to the measured one.…”

Section: Phase Retrieval Techniquementioning

confidence: 99%

Light sheet adaptive optics microscope for 3D live imaging

Bourgenot

Taylor

Saunter

et al. 2013

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX

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Simple characterisation of a deformable mirror inside a high numerical aperture microscope using phase diversity

Cited by 14 publications

References 13 publications

Calibration of an adaptive microscope using phase diversity

Calibration of an adaptive microscope using phase diversity

Adaptive optical microscopy: the ongoing quest for a perfect image

Light sheet adaptive optics microscope for 3D live imaging

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