Simple Approach for Species Discrimination of Fabaceae Family on the Basis of Length Variation in PCR Amplified Products Using Barcode Primers

Sikdar, Sumana; Tiwari, Sharad; Sapre, Swapnil; Thakur, Vishwa Vijay

doi:10.20546/ijcmas.2018.712.115

Cited by 2 publications

(4 citation statements)

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“…Further, among all DNA barcoding primers only three primer pairs (atpF-atpH, trnH-psbA and trnL) were able to produce amplicon length polymorphism with studied plant species and rest of the primers amplified only monomorphic alleles. Similar results were obtained in a recent study conducted to differentiate 24 representative species of Fabaceae family on the basis of amplicon length polymorphism (Sikdar et al 2018) where, rbcL and matK produced monomorphic band. To identify the specific species, we have developed a colour code matrix which may also be used for discriminating valuable medicinal plant species from their potential adulterants.…”

Section: Discussionsupporting

confidence: 89%

“…Identification of plant species based on amplicon length variability which can be visibly envisaged on agarose gel and does not necessitate sequencing; therefore, it is a decent and inexpensive approach to differentiate plant species (Sikdar et al 2018). This method can provide the way to sort adulteration in herbal drug materials in a normal facility molecular biology laboratory because of no need of sequencing of amplicons.…”

Section: Discussionmentioning

confidence: 99%

“…Although DNA barcoding is proven useful for identification of plants (Sikdar et al 2018) but the process of sequencing is costly and time taking which hinders the wider application of this technique in developing countries (Osathanunkul et al 2016). Hence, there is a need to develop reliable, cost effective, rapid and simple technique for proper identification of plant's taxa.…”

Section: Introductionmentioning

confidence: 99%

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Molecular identification of medicinal plants with amplicon length polymorphism using universal DNA barcodes of the atpF–atpH, trnL and trnH–psbA regions

et al. 2019

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Amplification success and species discrimination efficiency of universal DNA barcode primers (trnH-psbA, trnL, ycf1b, atpF-atpH, matK and rbcL) was evaluated in 46 representative medicinal plant species of 28 families on agarose gel. The results showed that amplicons length polymorphism revealed by the primers atpF-atpH, trnH-psbA and trnL can simultaneously discriminate all the 46 species under study precisely. Some of the plant species included in this study are used as potential adulterants to other plant species. We were able to successfully discriminate the plant species from their potential substitute. Vitex negundo is an adulterant of Ocimum sanctum which has been successfully discriminated by all these three markers. Another example of adulteration between Bacopa monnieri and Centella asiatica was successfully discriminated by atpF-atpH, trnL and trnH-psbA on the basis of variability in amplicon length of these two medicinal herbs. Further, Cassia tora and Cassia fistula are also adulterants for each other and variability in amplicon length between these two species was revealed by atpF-atpH and trnH-psbA markers. A colour code distance matrix based on amplicon length polymorphism was designed to select primers which can effectively discriminate plants species on the basis of their amplicon length. Discrimination of plant species with the universal markers on agarose gel is a noble and inexpensive approach as it does not require sequencing of amplicons. This procedure will provide a way for the development of diagnostic markers to identify adulteration not only in herbal drug formulations but also in food material.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular identification of medicinal plants with amplicon length polymorphism using universal DNA barcodes of the atpF–atpH, trnL and trnH–psbA regions

et al. 2019

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“…2,3 It is the second most important family in nutritional and economic aspects, behind only the family of cereals. 4 The genus Dalbergia is distributed mainly in tropical and subtropical regions of Africa, America, and Asia. In those continents, approximately 250 Dalbergia species have been cataloged.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Activity and Docking Studies of Cathepsin V for Isoflavanoids from Dalbergia miscolobium Benth

Silva¹,

Moreira²,

Cardoso³

et al. 2021

Rev. Virtual Quim.

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Resumo: Os extratos de plantas do gênero Dalbergia demonstram uma ampla gama de atividades biológicas, incluindo analgésica, antidiabética, anti-inflamatória e antimicrobiana. Neste trabalho, o estudo químico dos extratos das folhas e galhos de Dalbergia miscolobium levou ao isolamento e identificação de cinco isoflavonoides: prunetina, di-O-metildaidzeína, 8-O-metilretusina, duartina e sativan por meio de dados de ressonância magnética nuclear. A atividade de inibição enzimática desses Isoflavonoides foi avaliada na catepsina V na concentração de 100 µM. Duartin e sativan mostraram uma atividade notável contra a catepsina V, exibindo valores de inibição de 89% e 88%, respectivamente. Além disso, foram realizadas simulações de docking molecular para predizer o modo de ligação dos isoflavonoides a essa proteína e os resultados mostraram que a duartina está muito bem ligada à catepsina V e estabilizada por duas ligações de hidrogênio. Os isoflavanonoides duartina e sativan mostraram uma importante porcentagem de inibição da catepsina V, que pode ser considerada como alvo na investigação dos inibidores da catepsina V e estudos químicos adicionais de espécies de Dalbergia podem proporcionar novos isoflavonoides inibidores da catepsina V. Plant extracts from Dalbergia genus have demonstrated a wide range of biological activities including, analgesic, antidiabetic, anti-inflammatory, and antimicrobial. In this work, the chemical study of the extracts from the leaves and branches of Dalbergia miscolobium led to the isolation and identification of five isoflavonoids: prunetin, di-O-methyldaidzein, 8-O-methylretusin, duartin and sativan employing nuclear magnetic resonance data. The inhibition activity of these isoflavonoids was screened against cathepsin V at a concentration of 100 µM. Duartin and sativan showed remarkable activity against cathepsin V displaying 89% and 88% inhibition values, respectively. Also, docking simulations to predict the binding mode of isoflavonoids into this protein were performed and results showed that the duartin is nicely bound to the cathepsin V and stabilized by two hydrogen bonds. The isoflavans duartin and sativan showed a significant inhibition percentage of cathepsin V, which can be considered as targets into cathepsin V inhibitors investigation and further chemical study of Dalbergia species may afford novels isoflavonoids cathepsin inhibitors.

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Simple Approach for Species Discrimination of Fabaceae Family on the Basis of Length Variation in PCR Amplified Products Using Barcode Primers

Cited by 2 publications

References 11 publications

Molecular identification of medicinal plants with amplicon length polymorphism using universal DNA barcodes of the atpF–atpH, trnL and trnH–psbA regions

Molecular identification of medicinal plants with amplicon length polymorphism using universal DNA barcodes of the atpF–atpH, trnL and trnH–psbA regions

Inhibitory Activity and Docking Studies of Cathepsin V for Isoflavanoids from Dalbergia miscolobium Benth

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