Simple and Sensitive Detection of Bacterial Hydrogen Sulfide Production Using a Paper-Based Colorimetric Assay

Abstract

Hydrogen sulfide (H2S) is known to participate in bacteria-induced inflammatory response in periodontal diseases. Therefore, it is necessary to quantify H2S produced by oral bacteria for diagnosis and treatment of oral diseases including halitosis and periodontal disease. In this study, we introduce a paper-based colorimetric assay for detecting bacterial H2S utilizing silver/Nafion/polyvinylpyrrolidone membrane and a 96-well microplate. This H2S-sensing paper showed a good sensitivity (8.27 blue channel intensity/μM H2S, R2 = 0.9996), which was higher than that of lead acetate paper (6.05 blue channel intensity/μM H2S, R2 = 0.9959). We analyzed the difference in H2S concentration released from four kinds of oral bacteria (Eikenella corrodens, Streptococcus sobrinus, Streptococcus mutans, and Lactobacillus casei). Finally, the H2S level in Eikenella corrodens while varying the concentration of cysteine and treatment time was quantified. This paper-based colorimetric assay can be utilized as a simple and effective tool for in vitro screening of H2S-producing ability of many bacteria as well as salivary H2S analysis.

Combining PDMS Composite and Plasmonic Solid Chemosensors: Dual Determination of Ammonium and Hydrogen Sulfide as Biomarkers in a Saliva Single Test

“…Salivary Hydrogen Sulfide Measured with a New Highly Sensitive Self-Immolative Coumarin-Based Fluorescent Probe 0.05-0.56 0.02 [33] Sensitivity of salivary hydrogen sulfide to psychological stress and its association with exhaled nitric oxide and affect 0.08-0.56 0.02 [34] Simple and Sensitive Detection of Bacterial Hydrogen Sulfide Production Using a Paper-Based Colorimetric Assay 0.02-2.8 0.008 [35] Sub-Parts-per-Million Hydrogen Sulfide Colorimetric Sensor: Lead Acetate Anchored Nanofibers toward Halitosis Diagnosis 0.6-5 0.2 [36] Plasmonic sensor for hydrogen sulphide in saliva: Multisensor platform and bag format 0.06-1 0.02 [37] In this paper, a dual determination of NH4 + and H2S have been proposed in saliva samples using two patented chemosensors from the MINTOTA group [38,39]. The chemosensor for ammonium is based on a composite of PDMS, tetraethyl orthosilicate (TEOS), Silica NPs, ionic liquid (IL) and 1,2-naphthoquinone-4-sulfonate (NQS) as a chromophore [26,38].…”

“…The values found ranged from 0.055 to 0.3 mg/L. Ahn et al [35] developed a paper chemosensor impregnated with silver nitrate to detect H2S produced by various bacteria. Cha et al [36] created a colorimetric chemosensor based on lead acetate nanofibers as a possible halitosis diagnostic.…”

A novel “on–off–on” near-infrared fluorescent probe for Cu2+ and S2− continuous detection based on dicyanoisoflurone derivatives, and its application in bacterial imaging

“…Suldes mainly come from metabolites produced by the breakdown of organic matter by bacteria. [12][13][14] At the same time, the accumulation of suldes in the body can cause various health problems, such as loss of consciousness, respiratory paralysis, and digestive diseases. In addition, suldes are easily protonated to produce hydrogen sulde, 15 H 2 S plays an important role in the physiological and nervous system, [16][17][18] and a variety of pathological conditions such as diabetes, heart failure, hypertension, asthma, atherosclerosis, and Alzheimer's disease can occur under conditions exposed to high concentrations of H 2 S. 19 Therefore, the design and synthesis of a highly selective probe to detect Cu 2+ and S 2− in environmental and biological samples is of great practical signicance.…”