Simple and sensitive assay for nucleic acids by use of the resonance light-scattering technique with the anionic dye methyl blue in the presence of cetyltrimethylammonium bromide

Cai, Changqun; Gong, Hang; Chen, Xiaoming

doi:10.1007/s00604-006-0620-z

Cited by 8 publications

(3 citation statements)

References 24 publications

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“…15 Recently, RLS studies have attracted great interest among researchers due to the sensitivity, rapidity and simplicity of this technique. [16][17][18][19][20][21][22] The successful application of the RLS technique to directly screen DNA-targeted anticancer drugs in vitro has been demonstrated. [23][24][25] The RLS technique has already been employed for the detection of DNA hybridization.…”

Section: Introductionmentioning

confidence: 99%

Label-free detection of target DNA sequence and single-base mismatch in hepatitis C virus corresponding to oligonucleotide by resonance light scattering technique

et al. 2012

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Section: Introductionmentioning

confidence: 99%

Label-free detection of target DNA sequence and single-base mismatch in hepatitis C virus corresponding to oligonucleotide by resonance light scattering technique

et al. 2012

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“…Afterwards, Huang et al successfully employed RLS technique for biomacromolecule quantitative analysis [19,20]. Recently, RLS studies have attracted great interest among researchers due to the sensitivity, rapidity and simplicity of this technique [21][22][23][24][25][26][27][28][29]. In addition, the successful application of RLS technique to directly screen DNA-targeted anticancer drugs in vitro has been demonstrated [30,31].…”

Section: Introductionmentioning

confidence: 99%

Investigation on the interaction between anthracyclines and DNA in the presence of paclitaxel by resonance light scattering technique

et al. 2011

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We have developed a method to investigate the interaction between DNA-targeted anthracyclines and DNA in the presence of the drug paclitaxel. It is based on resonance light scattering (RLS) and on the finding that anthracyclines when bound to DNA undergo a dramatic enhancement in their RLS intensities, while paclitaxel does not display such an effect. However, the RLS intensities of the anthracyclines-DNA associates are remarkably enhanced again on addition of paclitaxel. UV-visible spectra reveal interactions between paclitaxel and anthracyclines, but no reaction between paclitaxel and DNA. Consequently, paclitaxel, though not DNAtargeted, can improve the DNA-binding capabilities of anthracyclines. Binding constants between anthracyclines and DNA, and improved efficiency of paclitaxel on the DNA-binding capabilities of anthracyclines were calculated. The DNA binding constants of doxorubicin, epirubicin, and mitoxantrone, respectively, are 4.53×10 5 L mol −1 , 6.05×10 5 L mol −1 , and 9.47×10 5 L mol −1 . The improved values in presence of paclitaxel are 78%, 47% and 19%. We also have investigated the effects of drug concentrations and the order of adding the drugs. Displacement studies (using methylene blue as a competitive agent) provided additional information on the mechanisms of the interaction between paclitaxel and anthracyclines.

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“…Due to its high sensitivity, selectivity, and convenience, RLS studies have attracted great interest among researchers. In recent years, RLS technique has been used to determine pharmaceutical [ 2 – 5 ], ion [ 6 ], bacteria [ 7 ], and various biological macromolecules, such as nucleic acids [ 8 – 10 ], proteins [ 11 – 15 ], and peptide [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive Determination of Trace Chloride Ion in Drinks Using Resonance Light Scattering Technique

Cao

2008

Journal of Automated Methods and Management in Chemistry

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A resonance light scattering (RLS) technique to determine chloride ions in drinks was developed. Chloride ions were found to bind Ag+ forming AgCl aggregates that produced intense resonance scattering light. Effects of factors such as acidity, ionic strength, and coexistent interferents on the RLS of AgCl aggregates were investigated. The pH of solution almost did not affect the production of RLS and few foreign species interfered with the detection of chloride ions. The resonance scattering light intensity at the maximum peak of 571 nm was linear to the concentration of chloride ions in the range of 1.42–8.52 ng mL−1 with a detection limit of 0.71 ng mL−1. To determine the feasibility of the proposed method, some samples of water and drinks were analyzed. The attained results were in agreement with that of ion-selective electrode method. Good recovery results were also obtained with the range of 94.08–105.63%. The sensitivity and selectivity of the RLS method are high enough to determine trace amounts of chloride ions without any significant interference from high concentration of other components such as common anions and cations.

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Simple and sensitive assay for nucleic acids by use of the resonance light-scattering technique with the anionic dye methyl blue in the presence of cetyltrimethylammonium bromide

Cited by 8 publications

References 24 publications

Label-free detection of target DNA sequence and single-base mismatch in hepatitis C virus corresponding to oligonucleotide by resonance light scattering technique

Label-free detection of target DNA sequence and single-base mismatch in hepatitis C virus corresponding to oligonucleotide by resonance light scattering technique

Investigation on the interaction between anthracyclines and DNA in the presence of paclitaxel by resonance light scattering technique

Rapid and Sensitive Determination of Trace Chloride Ion in Drinks Using Resonance Light Scattering Technique

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