Simple and reliable extraction and a validated high performance liquid chromatographic assay for quantification of amoxicillin from plasma

Purohit, Trusha J.; Wu, Zimei; Hanning, Sara M.

doi:10.1016/j.chroma.2019.460611

Cited by 9 publications

(12 citation statements)

References 28 publications

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“…The results showed that during 4 weeks of storage, the lyophilized form was kept at 4 °C and room temperature (25 °C) and possessed a slower degradation profile compared to the liquid form under the same storage conditions ( Figure 8 ). Therefore, based on the data obtained, the lyophilized form of in situ gelling nasal formulation appeared to be considered a suitable dosage form for the preparation of AMT with the 4 °C storage condition [ 93 ]. According to these results, the lyophilized form with a low temperature of storage (4 °C) is recommended to maintain the stability of AMT in nasal formulation until redispersion.…”

Section: Resultsmentioning

confidence: 99%

Development of Thermoresponsive-Gel-Matrix-Embedded Amoxicillin Trihydrate-Loaded Bovine Serum Albumin Nanoparticles for Local Intranasal Therapy

Mardikasari

Budai-Szűcs

Orosz

et al. 2022

Gels

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A high dose of amoxicillin is recommended as the first-line therapy for acute bacterial rhinosinusitis (ABR). However, oral administration of amoxicillin is connected to many adverse reactions coupled with moderate bioavailability (~60%). Therefore, this study aimed to develop a topical nasal preparation of amoxicillin, employing a thermoresponsive nanogel system to increase nasal residence time and prolong drug release. Rheological investigations revealed that formulations containing 21–23% w/w Poloxamer 407 (P407) were in accordance with the requirement of nasal administration (gelling temperature ~35 °C). The average hydrodynamic diameter (<200 nm), pH (6.7–6.9), and hypertonic osmolality (611–663 mOsmol/L) of the in situ gelling nasal nanogel appeared as suitable characteristics for local rhinosinusitis treatment. Moreover, taking into account the mucoadhesive strength and drug release studies, the 21% w/w P407 could be considered as an optimized concentration for effective nasal delivery. Antibacterial activity studies showed that the ability of amoxicillin-loaded in situ gelling nasal nanogel to inhibit bacterial growth (five common ABR pathogens) preserved its effectiveness in comparison to 1 mg/mL amoxicillin aqueous solution as a positive control. Altogether, the developed amoxicillin-loaded in situ gelling thermoresponsive nasal nanogel can be a potential candidate for local antibiotic therapy in the nasal cavity.

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Section: Resultsmentioning

confidence: 99%

Development of Thermoresponsive-Gel-Matrix-Embedded Amoxicillin Trihydrate-Loaded Bovine Serum Albumin Nanoparticles for Local Intranasal Therapy

Mardikasari

Budai-Szűcs

Orosz

et al. 2022

Gels

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“…Blank samples spiked with 1, 5, 20, 50, and 100 μg kg −1 for ZEN and 5, 10, 20, 50, and 100 μg kg −1 for ZEN-14G were utilized to investigate the recovery, intra-day, and inter-day precisions ( n = 5). The recovery of the overall process efficiency ( PE ) was calculated by comparing the concentration levels measured by the matrix-matched calibration curves with the spiked levels according to the following formula ( Purohit et al, 2020 ). Recovery values between 70 and 120% were considered to be acceptable.…”

Section: Methodsmentioning

confidence: 99%

Development of a reliable UHPLC-MS/MS method for simultaneous determination of zearalenone and zearalenone-14-glucoside in various feed products

et al. 2022

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A reliable ultra-high-performance liquid chromatography–tandem mass spectrometry method (UHPLC-MS/MS) was developed for the simultaneous determination of two mycotoxins, that is, zearalenone (ZEN) and zearalenone-14-glucoside (ZEN-14G) in formula feed, concentrated feed, and premixed feed products. An improved sample pretreatment was achieved with the hydrophilic–lipophilic balance (HLB) cartridges efficiently removing the impurities and enriching the target analytes in different feeds. The critical parameters affecting the performance of the solid-phase extraction (SPE) procedure were carefully optimized, and 20% acetonitrile in water as the loading solution, 50% methanol in water as the washing solvent, and 5 ml of methanol as the elution solvent yielded the optimal purification efficiencies. The established method was thoroughly validated in terms of linearity (R2 ≥ 0.999), sensitivity (limit of quantification in the range of 0.50–5.00 μg kg−1), recovery (89.35 ± 2.67% to 110.93 ± 1.56%), and precision (RSD, 3.00–14.20%), and it was then successfully applied to investigate a total of 60 feed samples. Among them, 50 samples were found to be contaminated with ZEN (an incidence of 83.3%) at levels ranging from 0.63 to 615.24 μg kg−1, whereas 22 samples were contaminated with ZEN-14G (an incidence of 36.7%) in the range of 0.89–15.31 μg kg−1. The developed method proved to be a specific and reliable tool for intensive monitoring of ZEN and ZEN-14G in complex feed matrices.

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“…In order to obtain the most effective method, first, three different columns were study as a stationary phase, then different rations of mobile phase and different flow rates were tested. The ICH guideline was used to validate the developed HPLC method following the parameters specified by it [13,[15][16][17]. 2.6.1.…”

Section: Methods Development and Validationmentioning

confidence: 99%

Determination of Enalapril maleate from tablets using a new HPLC method

Gherman

Zavastin

Spac

et al. 2021

Ovidius University Annals of Chemistry

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For the determination of enalapril maleate in tablets a new, simple and economical HPLC method was developed and fully validated. Chromatographic separation was achieved on Hewlett Zorbax SB-C 18 (150 x 4.6 mm, 5 μm) column and the mobile phase consisted of acetonitrile: 0.025 M phosphate buffer adjusted to pH 3 (70:30 v/v) pumped at a flow rate 0.8 mL/min and UV-detection was performed at 210 nm. The proposed method was validated according to ICH guidelines (linearity, limit of detection, limit of quantification, precision, accuracy, recovery and system suitability). The total run time was less than 3 min and the retention time for Enalapril maleate was 2.3 min. The calibration graph was linear in the concentration range between 10 – 100 μg/mL with the correlation coefficient r2 = 0.9998. The developed and validated method was successfully applied to determine the Enalapril maleate in tablets. Therefore, this method proved to be sensitive, specific and reproducible and can be applied for routine analysis of enalapril maleate from pharmaceutical formulation due to its simplicity of application.

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Simple and reliable extraction and a validated high performance liquid chromatographic assay for quantification of amoxicillin from plasma

Cited by 9 publications

References 28 publications

Development of Thermoresponsive-Gel-Matrix-Embedded Amoxicillin Trihydrate-Loaded Bovine Serum Albumin Nanoparticles for Local Intranasal Therapy

Development of Thermoresponsive-Gel-Matrix-Embedded Amoxicillin Trihydrate-Loaded Bovine Serum Albumin Nanoparticles for Local Intranasal Therapy

Development of a reliable UHPLC-MS/MS method for simultaneous determination of zearalenone and zearalenone-14-glucoside in various feed products

Determination of Enalapril maleate from tablets using a new HPLC method

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