Simple and rapid screening of entomopathogenic fungi having radical-scavenging and anticancer activities

Shin, Tae Young; Bae, Sung Min; Choi, Jae Bang; Woo, Soo Dong

doi:10.1016/j.aspen.2014.05.002

Cited by 7 publications

(5 citation statements)

References 12 publications

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“…The metabolites produced from entomopathogenic fungi can be used in various fields, including in the development of insecticidal, antimicrobial, anticancer, antioxidant, and antiviral materials (Jiang et al 2008;Shin et al 2014). Furthermore, mice treated with P. tenuipes extracts showed improved lipid and glucose metabolic parameters compared to those without the treatment, which is similar to the effect observed in mice treated with Cordyceps militaris extracts (Deng et al 2020).…”

Section: Introductionmentioning

confidence: 66%

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Effects of Paecilomyces tenuipes‐inoculated brown rice combined with fermented oak sawdust on the developmental growth of Protaetia brevitarsis larvae

Kwak

Kim

et al. 2022

Entomological Research

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Protaetia brevitarsis is an edible insect registered as an alternative food ingredient by the Korean Ministry of Food and Drug Safety. Herein, we investigated the effects of Paecilomyces tenuipes on diseases reported in the larvae of edible insects with the aim of improving the productivity of larvae by promoting their development and enhancing their disease resistance. P. tenuipes exhibits several biological and pharmaceutical properties. We developed the feed for P. brevitarsis larvae with fermented oak sawdust combined with P. tenuipes–inoculated brown rice. The larval group that reared on feed combined with P. tenuipes showed a 6.5‐fold increase in weight and 5‐fold increase in survival rate against Metarhizium anisopliae relative to that seen with the controls. The nutrient composition of this feed formulation was 2.37 times higher in crude protein than in the other feed types, including the control. Our results suggest that P. tenuipes–inoculated brown rice can be used to increase larval weight and promote disease resistance, thereby potentially reducing the prevalence and severity fungal disease caused by M. anisopliae.

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Section: Introductionmentioning

confidence: 66%

“…2008; Shin et al . 2014). Furthermore, mice treated with P. tenuipes extracts showed improved lipid and glucose metabolic parameters compared to those without the treatment, which is similar to the effect observed in mice treated with Cordyceps militaris extracts (Deng et al .…”

Section: Introductionmentioning

confidence: 99%

Effects of Paecilomyces tenuipes‐inoculated brown rice combined with fermented oak sawdust on the developmental growth of Protaetia brevitarsis larvae

Kwak

Kim

et al. 2022

Entomological Research

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“…Strain isolation and culture referred to Shin TY's method [12]. The pileus of the fresh mushroom was cut off with a scissor and placed on a filter paper, covered with a beaker for 24h, and the spores were collected.…”

Section: Strain Isolation and Culturementioning

confidence: 99%

Screening, Isolation and Identification of High Antioxidant Strains from 16 Kinds of Mushrooms

Pei-Xia

Tian-Tian

et al. 2020

MRJI

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Aims: To promote the rational exploration of the mushroom resources, this study evaluated the in vitro antioxidant activity of 16 kinds of mushrooms collected from the Sichuan province in China and completed the identification of the optimal active strains. Place and Duration of Study: School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan province, China. April 2019 to May 2020 (13 Months). Methodology: 16 kinds of mushrooms were collected in the wild. These strains were isolated and cultivated. The extract’s antioxidant activities of fermentation broth and mycelium were assessed using the DPPH radical-scavenging method. The higher activity strains were also evaluated by Trolox equivalent antioxidant capacity (TEAC) and the Ferric reducing antioxidant power (FRAP) assay. Meanwhile, the contents of total flavonoids and total phenols were determined. Finally, The highly active strains were identified by internal transcribed spacer (ITS) Identification. Results: All extract of fermentation broth and mycelium from 16 kinds of mushrooms had scavenging effects on DPPH. Among them, strain No. 6 and No. 7 exhibited obvious antioxidant capacity, and the scavenging rates of their fermentation broths on DPPH were 89.47% and 84.27%, respectively. And the results of TEAC and FRAP were consistent with those of DPPH. Besides, these two kinds of mushrooms were all rich in phenolic and flavonoid compounds, and the content in the fermentation broth was significantly higher than that in the mycelium, indicating that phenolic and flavonoid substances may be the material basis of their antioxidant effect. The strain No. 6 and No. 7 were confirmed to be Bjerkandera and Nigrospora sp, respectively by 16S nrDNA sequence identification. Conclusion: The results showed that Bjerkandera and Nigrospora sp. possessed the highest antioxidant capacities with rich total phenolic and total flavonoids. They could be potential sources of natural antioxidants for further exploration and application.

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“…In recent years entomopathogenic fungal metabolites have attracted attention since they exhibit a wide variety of insecticidal, antimicrobial, anticancer, antioxidant, and antiviral activities. Furthermore, they have been suggested as potential candidates for the development of new bioactive agents [20]. It is also well established that many species of entomopathogenic fungus such as Isaria farinosa possesses anti-oxidative properties as well as the capacity for inhibiting cancer growth [21].…”

Section: Introductionmentioning

confidence: 99%

Isaria tenuipes Peck, an entomopathogenic fungus from Darjeeling Himalaya: Evaluation of in-vitro antiproliferative and antioxidant potential of its mycelium extract

Chhetri

Shahi

et al. 2020

BMC Complement Med Ther

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Background: Isaria tenuipes is one of the potent species in the members of the genus Isaria, which is well reported to possess multiple bioactive substances of therapeutic importance. Therefore, an in vitro experimental study was carried to evaluate the bioactivities of the crude methanolic extract from the mycelium of this fungus. Methods: The fungus was authenticated through morphological characters and the species discrepancy was resolved using the nuclear rDNA ITS sequence. The methanolic extract was fingerprinted by FTIR. The antioxidant components in terms of total phenols and flavonoids were determined as gallic acid and quercetin equivalents respectively. Antioxidant activities of the methanolic extract was assessed using 1, 1-diphenyl-2-picrylhydrazyl (DPPH), 2, 2 /-azinobis-(3-ethylbenzthiazoline-6-sulphonic acid) radical cation (ABTS 0+), Fe 2+ chelating activity, and hydroxyl radical scavenging assays. Cytotoxicity of the extract was determined by [3-(4, 5-dimethylthiazol-2-yl)-2, 5diphenyltetrazolium bromide] (MTT) assay on three cancer cell lines: HeLa, HepG2, and PC3. Apoptosis was further studied by propidium iodide (PI) and Annexin-V/PI staining flow cytometric analysis. Anti-proliferation capacity was studied by colony-forming assay.

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Simple and rapid screening of entomopathogenic fungi having radical-scavenging and anticancer activities

Cited by 7 publications

References 12 publications

Effects of Paecilomyces tenuipes‐inoculated brown rice combined with fermented oak sawdust on the developmental growth of Protaetia brevitarsis larvae

Effects of Paecilomyces tenuipes‐inoculated brown rice combined with fermented oak sawdust on the developmental growth of Protaetia brevitarsis larvae

Screening, Isolation and Identification of High Antioxidant Strains from 16 Kinds of Mushrooms

Isaria tenuipes Peck, an entomopathogenic fungus from Darjeeling Himalaya: Evaluation of in-vitro antiproliferative and antioxidant potential of its mycelium extract

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