Simple and Rapid Method for Detecting Biofilm Forming Bacteria

Kalia, Vipin Chandra; Prakash, Jyotsana; Koul, Shikha; Ray, Subhasree

doi:10.1007/s12088-016-0616-2

Cited by 39 publications

(10 citation statements)

References 14 publications

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“…A link between the biofilm forming ability of bacteria and successful colonization and functions of gut flora is suggested [47,48]. The ability to form biofilms helps bacteria to survive in hostile environment offered by the GI tract and is seen in cases of both pathogenic and probiotic bacteria [49,50]. To unravel the allochthonous communities, fecal samples are generally examined while mucosal epithelium scraped after rinsing are used for analyzing the adherent residents.…”

Section: Fate Of Colonizing Members and The Factors Affecting Fish Gumentioning

confidence: 99%

Fish Gut Microbiome: Current Approaches and Future Perspectives

et al. 2018

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In recent years, investigations of microbial flora associated with fish gut have deepened our knowledge of the complex interactions occurring between microbes and host fish. The gut microbiome not only reinforces the digestive and immune systems in fish but is itself shaped by several host-associated factors. Unfortunately, in the past, majority of studies have focused upon the structure of fish gut microbiome providing little knowledge of effects of these factors distinctively and the immense functional potential of the gut microbiome. In this review, we have highlighted the recently gained insights into the diversity and functions of the fish gut microbiome. We have also delved on the current approaches that are being employed to study the fish gut microbiome with an aim to collate all the knowledge gained and make accurate conclusions for their application based perspectives. The literature reviewed indicated that the future research should shift towards functional microbiomics to improve the maximum sustainable yield in aquaculture.

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Section: Fate Of Colonizing Members and The Factors Affecting Fish Gumentioning

confidence: 99%

Fish Gut Microbiome: Current Approaches and Future Perspectives

et al. 2018

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“…recA [17]. Though Staphylococcus is a natural microflora, yet it is a source of potentially dangerous nosocomial infections caused by antibiotic resistant strains that form rigid biofilms which can be targeted by exploring quorum quenching mechanism [18][19][20][21][22][23]. Staphylococcus warneri contained multiple proteins responsible for complete sperm agglutination.…”

Section: Discussionmentioning

confidence: 99%

Contraceptive Sperm Agglutinating Proteins Identified in Staphylococcus warneri, Natural Microflora of an Infertile Woman

et al. 2018

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Staphylococcus warneri, isolated from the cervix of an adult female with unexplained infertility, was found to agglutinate human spermatozoa in vitro leading to their death. A genomic library of S. warneri was generated using pSMART-Escherichia coli vector-host system. Approximately 3500 transformants were screened and four showed sperm agglutinating activity. Sperm agglutinating proteins (SAPs) were partially purified from the positive transformants and were found to agglutinate sperms in vitro. Cloned ORFs in positive transformants were sequenced and ORF finder identified them as endonuclease, accessory secretory protein-Asp1, accessory secretory protein-Asp2 and signal transduction protein. Mannose was found to competitively inhibit sperm agglutination, indicating that SAPs in S. warneri bind to mannose in glycoprotein receptors on the surface of sperms for agglutination. This is the first report on identification of SAPs which may be responsible for unexplained infertility in women and may be used as contraceptive agents.

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“…The biofilm formation of KCCM 43270 was determined using crystal violet staining as described by Dawson, Valiente, Faulds‐Pain, Donahue, and Wren () and Kalia, Prakash, Koul, and Ray (). About 1% (v/v) of overnight grown culture was inoculated into 5 ml sterile casein‐mannitol (CM) medium (casein digest 20 g/L and d ‐mannitol 20 g/L).…”

Section: Methodsmentioning

confidence: 99%

“…The biofilm formation of KCCM 43270 was determined using crystal violet staining as described by Dawson, Valiente, Faulds-Pain, Donahue, and Wren (2012) and Kalia, Prakash, Koul, and Ray (2017).…”

Section: Biofilm Formationmentioning

confidence: 99%

Autochthonous Bacillus licheniformis: Probiotic potential and survival ability in low‐fishmeal extruded pellet aquafeed

et al. 2018

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In recent years, Bacillus spp. have garnered attention as probiotic supplements in aquafeed owing to the production of heat stable and low pH resistant spores. Herein, we isolated and characterized an autochthonous Bacillus licheniformis KCCM 43270 from the intestine of olive flounder ( Paralichthys olivaceus ) for supplementation in low‐fishmeal extruded aquafeeds. The KCCM 43270 was screened based on amylase, protease, cellulase, and lipase as well as non‐hemolytic activities. The isolate was able to grow in carboxymethyl cellulose (CMC), xylan, and soybean meal (SBM) when used as a single carbon source in the minimal nutrient M9 medium. The KCCM 43270 spores displayed complete survival in acid (pH 2.5) and bile (0.3%, w/v) for 3 hr, strong biofilm formation, and nearly 50% adhesion with intestinal mucus. The spores of the isolate also showed significant survival ability at 80, 90, 100°C for 60, 30, and 1 min, respectively. In addition, the spores in a blend of SBM complex carrier showed significant heat stability at 120°C for 5 min and under different drying conditions. Furthermore, the spores also survived the extrusion process during low‐fishmeal aquafeed manufacturing, implying the potential application of B. licheniformis KCCM 43270 in aquafeed industry.

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Simple and Rapid Method for Detecting Biofilm Forming Bacteria

Cited by 39 publications

References 14 publications

Fish Gut Microbiome: Current Approaches and Future Perspectives

Fish Gut Microbiome: Current Approaches and Future Perspectives

Contraceptive Sperm Agglutinating Proteins Identified in Staphylococcus warneri, Natural Microflora of an Infertile Woman

Autochthonous Bacillus licheniformis: Probiotic potential and survival ability in low‐fishmeal extruded pellet aquafeed

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