Simple and Rapid Method for the Determination of Uric Acid-Independent Antioxidant Capacity

Duplančić, Darko; Kukoc-Modun, Lea; Modun, Darko; Radić, Njegomir

doi:10.3390/molecules16087058

Cited by 54 publications

(47 citation statements)

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“…Uric acid is a well-known antioxidant that contributes significantly to the plasma antioxidant capacity. These findings are in accordance with previous studies reporting that UA is contributing to approximately 70% of plasma total antioxidant capacity, when measured by FRAP method [24] . In addition, a negative correlation was observed between UA and progesterone level (r= -0.48, P<0.01) in the present study.…”

Section: Discussionsupporting

confidence: 83%

“…The correlation of some antioxidants with oxidative status indicates that antioxidative mechanisms are activated to cope with oxidative stress in physiologic conditions. On the other word, an increased antioxidant capacity in plasma may not necessarily be a desirable condition if it reflects a response to increased oxidative stress, or vice versa in physiologic condition such as the estrous cycle [24] .…”

Section: Discussionmentioning

confidence: 99%

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The Effect of Estrous Cycle on Oxidant and Antioxidant Parameters in Dairy Cows

Aydılek¹,

Varışlı²,

Selek³

et al. 2014

Kafkas Univ Vet Fak Derg

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Reactive oxygen species have fundamental roles in reproductive functions. To comprehensively evaluate the relation between reactive oxygen species and infertility, physiological variations across the estrous cycle in healthy cows have to be known. For this purpose 25 healthy multiparous Holstein dairy cows having regular estrous cycles were used. The estrous cycles were synchronized by ovsynch protocol. Oxidant [lipid hydroperoxide (LOOH), total oxidant status (TOS), oxidative stress index (OSI)], antioxidant parameters [total antioxidant status (TAS), total free sulfhydryl groups (SH), ceruloplasmin (CP), paraoxonase-1 (PON1), arylesterase (ARE), uric acid (UA)], lipid profile and progesterone levels were assayed at estrus, metestrus, diestrus and proestrus stages of the estrous cycle in the plasma samples. The plasma levels of oxidant (LOOH, TOS and OSI) and antioxidant (TAS, SH and UA) parameters were significantly decreased during the luteal phase compared to the follicular phase (at proestrus and at estrus) of the estrous cycle. There was also a significant positive correlation between TAS and TOS. The activity of PON1 and ARE significantly increased only at diestrus. Levels of high density lipoprotein, low density lipoprotein and total cholesterol elevated during the follicular phase (estrus) and declined during the luteal phase. In conclusion, oxidant/antioxidant status and lipid profile were affected by cyclic changes. Moreover, antioxidant defense system showed adaptive response to increased oxidative activities by occurring parallel increases and it may indicate that there is a dynamic balance between oxidant and antioxidant status during the estrous cycle in healthy cows. Keywords: Cow, Estrous cycle, Lipid peroxide, Lipid profile, Total antioxidant status, Total oxidant status Sütçü İneklerde Östrus Siklusunun Oksidan ve Antioksidan Parametreler Üzerine Etkisi ÖzetReaktif oksijen türlerinin üreme fonksiyonları üzerinde önemli rolleri vardır. Sağlıklı süt sığırlarında reaktif oksijen türleri ve infertilite arasındaki ilişkiyi kapsamlı bir şekilde değerlendirmek için östrus siklusu boyunca reaktif oksijen türü seviyelerindeki değişimlerin bilinmesi gerekir. Bu çalışmada düzenli siklus gösteren 25 adet sağlıklı Holştayn ırkı sütçü inek kullanıldı. Östrus siklusları ovsynch protokolü ile senkronize edildi. Östrus siklusunun proöstrus, östrus, metaöstrus ve diöstrus dönemlerinde kan plazmasında oksidan parametreler [lipit hidroperoksit (LOOH), total oksidan seviye (TOS), oksidatif stres indeksi (OSI)], antioksidan parametreler [total antioksidan kapasite (TAS), total serbest sülfhidril gruplar (SH), seruloplazmin (CP), paraoksonaz-1 (PON1), arilesteraz (ARE), ürik asit (UA)], lipit profili ve progesteron seviyesi ölçüldü. Foliküler dönemle karşılaştırıldığında luteal dönemdeki plazma oksidan (LOOH, TOS, OSI) ve antioksidan (TAS, SH, UA) seviyelerinin önemli derecede düştüğü görüldü. Ayrıca TAS ve TOS değerleri arasında önemli bir pozitif korelasyon vardı. Paraoksonaz-1 ve ARE aktiviteleri sadece diöstrus ...

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

The Effect of Estrous Cycle on Oxidant and Antioxidant Parameters in Dairy Cows

Aydılek¹,

Varışlı²,

Selek³

et al. 2014

Kafkas Univ Vet Fak Derg

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“…Serum UA was measured using the automatic analyzer (Hitachi 902, Roche). Total antioxidant capacity and the UA‐dependent antioxidant capacity (UA‐AC) were determined in serum and supernatant samples, in the absence or presence of uricase (Sigma Aldrich) treatment, respectively, by using the ferric reducing antioxidant power assay …”

Section: Methodsmentioning

confidence: 99%

Donor‐specific individuality of red blood cell performance during storage is partly a function of serum uric acid levels

et al. 2017

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BACKGROUND Previous investigations in leukoreduced units of red blood cells (RBCs) in mannitol additive solution revealed the close association of uric acid (UA) levels in vivo with the susceptibility of RBCs to storage lesion markers. In this study, we examined whether UA has a similar correlation with the capability of RBCs to cope with the oxidative provocations of storage under different conditions, namely, in CPDA‐1 and in the absence of leukoreduction. STUDY DESIGN AND METHODS The UA‐dependent antioxidant capacity of the supernatant was measured in nonleukoreduced units of RBCs in CPDA (n = 47). The possible effect of UA variability on the storage lesion profile was assessed by monitoring several physiologic properties of RBCs and supernatant, including cell shape, reactive oxygen species, and size distribution of extracellular vesicles, in units exhibiting the lowest or highest levels of UA activity (n = 16) among donors, throughout the storage period. RESULTS In stored RBC units, the UA‐dependent antioxidant activity of the supernatant declined as a function of storage duration but always in strong relation to the UA levels in fresh blood. Contrary to units of poor‐UA activity, RBCs with the highest levels of UA activity exhibited better profile of calcium‐ and oxidative stress–driven modifications, including a significant decrease in the percentages of spherocytes and of 100‐ to 300‐nm‐sized vesicles, typically associated with the exovesiculation of stored RBCs. CONCLUSION The antioxidant activity of UA is associated with donor‐specific differences in the performance of RBCs under storage in nonleukoreduced CPDA units.

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“…The total antioxidant capacity (TAC) was measured by the ferric‐reducing antioxidant power assay . The contribution of UA to the TAC was determined by uricase treatment . Protein carbonylation, NO, and clusterin were measured by ELISA kits according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%