Simple and rapid high-performance liquid chromatography analysis of propentofylline and its main metabolites in serum using a direct injection technique

Abstract

A rapid, simple and reliable high‐performance liquid chromatography (HPLC) column‐switching method with UV detection (270 nm) for the simultaneous determination of propentofylline and its metabolites in human and rat sera was developed. The method involves direct injection of serum onto an HPLC column, which contains a shielded hydrophobic stationary phase for the separation of analytes from proteins in serum, and then loading the analytes onto a short octadecylsilylated silica gel (ODS) column using a switching valve. Propentofylline and its three metabolites in serum were separated from the serum components within 30 min after the injection. The detection limits (S/N = 3) of analytes spiked in human and rat sera ranged from 0.08 to 0.57 nmol/mL, and the net volume of serum used was 20 µL. The relative standard deviations for within‐ and between‐day variations using rat serum were less than 4.3 and 5.6%, respectively. The method was used to determine propentofylline and its main metabolites in rat serum after a single intravenous dose of propentofylline (5 mg/kg). Copyright © 1999 John Wiley & Sons, Ltd.

Restricted access materials and large particle supports for on-line sample preparation: an attractive approach for biological fluids analysis

“…In the case of RAM sorbents, TAT depends not only on the separation time but also on the extraction time. Indeed, conventional flow rates are used for sample extraction on RAM supports and total analysis time are usually longer to 5 min [42,43,67,78,88,95,108,123,140]. Nevertheless, alkyl-diol-silica material have, recently, been found suitable for a fast and efficient sample extraction at a high flow rate [16,167].…”

“…Hisep column can tolerate 16 ml of serum without significant loss of performance [136]. Hisep material was used for the direct analysis of endogenous compounds [137] and pharmaceuticals in biological matrices [101,135,136,[138][139][140][141][142][143][144].…”

Antiallodynic effects of propentofylline Elicited by interrupting spinal glial function in a rat model of bone cancer pain

“…The alleviation of pain behavior in bone cancer pain by PPF may employ a complex downstream signaling pathway. The half‐life of PPF in blood is very short (<1 hr), so bioavailability is low following oral administration (Kuroda et al,1999). In vivo studies show that maintaining a certain concentration is necessary for the function of PPF (Raghavendra et al,2003, 2004a).…”

Development and validation of bioanalytical liquid chromatography–tandem mass spectrometry method for the estimation of pentoxifylline in human plasma: Application for a comparative pharmacokinetic study

“…4,5 As per the literature, there were many HPLC methods reported for the estimation of PTF using UV detection in biological matrix. [6][7][8][9][10][11][12][13][14][15][16][17][18][19] However, sensitivity, resolution, and time must be sacrificed by these conventional methods. There were few LC-MS methods proposed for quantification of PTF alone or along with its metabolites.…”