Simple and rapid gas-chromatographic determination of volatile metabolites in fermentation broths

Griot, M.; Dettwiler, B.; Heinzle, Elmar; Mayer, Francieli Martins; Dunn, Irving J.

doi:10.1016/s0003-2670(00)81335-5

Cited by 4 publications

(2 citation statements)

References 23 publications

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“…The result of this study might also be of some value for fermentation monitoring on which there is a very large literature 14–16. In this regard, infrared gas analysers have been used for the fast determination of kinetic parameters for ethanol production from glucose14 and on‐line computers have been used to facilitate data acquisition since they became readily available 15.…”

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confidence: 98%

“…In this regard, infrared gas analysers have been used for the fast determination of kinetic parameters for ethanol production from glucose14 and on‐line computers have been used to facilitate data acquisition since they became readily available 15. Also gas chromatography is used to monitor volatile metabolites from fermentation broth 16. Thus, SIFT‐MS with its facility for reliable on‐line quantification could well be used in these applications.…”

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Kinetics and isotope patterns of ethanol and acetaldehyde emissions from yeast fermentations of glucose and glucose‐6,6‐d₂ using selected ion flow tube mass spectrometry: a case study

Smith

Španěl

2001

Rapid Comm Mass Spectrometry

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As a prelude to investigations of the emission of metabolites from human cell lines in vitro, we have conducted a study using selected ion flow tube mass spectrometry (SIFT-MS) of the acetaldehyde and ethanol that appear in the headspace above a fermenting yeast/glucose/water mixture in sealed glass bottles at a temperature of 30 degrees C. A fixed quantity of yeast (10 mg) and varying amounts (2, 4, 8 and 16 mg) of both non-deuterated glucose and glucose-6,6-d2 in 5 mL of water were used and the emission of the acetaldehyde and the ethanol were observed as a function of time. The ethanol and acetaldehyde concentrations in the headspace were obtained from the magnitudes of their characteristic ions on the accumulated SIFT mass spectra and, when the deuterated glucose was used, characteristic singly and doubly deuterated ions were obvious. This study indicates, as expected, that ethanol is the major species generated and that acetaldehyde is a relatively minor component of the headspace and a very minor component of the liquid phase. We estimate that about 10(8) ethanol molecules are produced per minute per cell in this yeast fermentation process. The distribution of the non-deuterated and partially deuterated ethanol under these fermentation conditions is observed to be C2H5OH (66 +/- 4)%, C2H4DOH(6 +/- 1)%, C2H3D2OH(28 +/- 4)%, and the analogous distribution for the acetaldehyde is the same, within error. These results indicate that the D atoms in the glucose-6,6-d2 are mostly retained by the 6-C atom, but the appearance of the singly deuterated ethanol and acetaldehyde indicates that some D/H mixing must be occurring in the enzymatic reactions. The results of this study illustrate the potential and power of on-line SIFT-MS analysis in this area of research.

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Kinetics and isotope patterns of ethanol and acetaldehyde emissions from yeast fermentations of glucose and glucose‐6,6‐d₂ using selected ion flow tube mass spectrometry: a case study

Smith

Španěl

2001

Rapid Comm Mass Spectrometry

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Literaturverzeichnis

Schügerl¹

1997

Bioreaktionstechnik: Bioprozesse Mit Mikroorganismen Und Zellen

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Progress in monitoring, modeling and control of bioprocesses during the last 20 years

Schügerl

2001

Journal of Biotechnology

238

112

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Simple and rapid gas-chromatographic determination of volatile metabolites in fermentation broths

Cited by 4 publications

References 23 publications

Kinetics and isotope patterns of ethanol and acetaldehyde emissions from yeast fermentations of glucose and glucose‐6,6‐d₂ using selected ion flow tube mass spectrometry: a case study

Kinetics and isotope patterns of ethanol and acetaldehyde emissions from yeast fermentations of glucose and glucose‐6,6‐d₂ using selected ion flow tube mass spectrometry: a case study

Literaturverzeichnis

Progress in monitoring, modeling and control of bioprocesses during the last 20 years

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Simple and rapid gas-chromatographic determination of volatile metabolites in fermentation broths

Cited by 4 publications

References 23 publications

Kinetics and isotope patterns of ethanol and acetaldehyde emissions from yeast fermentations of glucose and glucose‐6,6‐d2 using selected ion flow tube mass spectrometry: a case study

Kinetics and isotope patterns of ethanol and acetaldehyde emissions from yeast fermentations of glucose and glucose‐6,6‐d2 using selected ion flow tube mass spectrometry: a case study

Literaturverzeichnis

Progress in monitoring, modeling and control of bioprocesses during the last 20 years

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Product

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Kinetics and isotope patterns of ethanol and acetaldehyde emissions from yeast fermentations of glucose and glucose‐6,6‐d₂ using selected ion flow tube mass spectrometry: a case study

Kinetics and isotope patterns of ethanol and acetaldehyde emissions from yeast fermentations of glucose and glucose‐6,6‐d₂ using selected ion flow tube mass spectrometry: a case study