Simple and rapid detection of the porcine reproductive and respiratory syndrome virus from pig whole blood using filter paper

Inoüe, Ryo; Tsukahara, Takamitsu; Sunaba, Chinatsu; Itoh, Mitsugi; Ushida, Kazunari

doi:10.1016/j.jviromet.2006.11.030

Cited by 28 publications

(32 citation statements)

References 17 publications

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“…Figure 3 shows the antigenicity plots of the structural proteins (GP2, GP3, GP4, GP5, M, and N) of the CA strain. As previously reported, the N protein was highly antigenic, which therefore makes it a suitable candidate for detection with virus-specific antibodies and diagnosis of the disease (Seuberlich et al, 2002;Inoue et al, 2007). However, the most important glycoprotein of PRRSV involved in the generation of PRRSV-neutralizing antibodies and protective immunity is the GP5 protein (Bastos et al, 2004;Jiang et al, 2006).…”

Section: J Microbiolmentioning

confidence: 91%

Molecular characterization of ORFs 2 to 7 of Korean porcine reproductive and respiratory syndrome virus (CA) and its protein expression by recombinant baculoviruses

Koo

Lee

et al. 2008

J Microbiol.

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To determine the characteristics of the Korean porcine reproductive and respiratory syndrome virus (PRRSV), CA, which was isolated from the serum of an infected pig in 2006, we investigated the nucleotide sequence and expression of the structural ORFs (ORFs 2 to 7) using the bApGOZA system. We found that the structural ORFs 2 to 7 of CA consisted of 3188 nucleotides that were the same as those formed from VR-2332. Comparison of the CA with the other strains revealed nucleotide sequence identity ranging from 89.8 to 99.5%. To better understand the genetic relationships between other strains, phylogenetic analyses were performed. The CA strain was closely related to the other North American genotype strains but formed a distinct branch with high bootstrap support. Additionally, expression levels of the PRRSV proteins in insect cells were strong or partially weak. The results of this study have implications for both the taxonomy of PRRSV and vaccine development.

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Section: J Microbiolmentioning

confidence: 91%

Molecular characterization of ORFs 2 to 7 of Korean porcine reproductive and respiratory syndrome virus (CA) and its protein expression by recombinant baculoviruses

Koo

Lee

et al. 2008

J Microbiol.

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“…The ORF7, encoding nucleocapsid (N) protein, is most consistent in nucleotide sequence between different strains of PRRSV [23], and has been presumed as the most useful marker for revealing the genetic relationships of PRRSV [24]. Alignment analysis of ORF7 deduced aa sequences indicates one aa substitution (Q17 ?…”

Section: Discussionmentioning

confidence: 99%

Genomic analysis of two Chinese strains of porcine reproductive and respiratory syndrome viruses with different virulence

Xue

LianXiang

et al. 2010

Virus Genes

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Two strains of porcine reproductive and respiratory syndrome virus (PRRSV) were isolated, designated GDQJ and GDBY1. Experimental inoculation showed that GDBY1, caused 100% morbidity and 67% mortality, while GDQJ, caused 100% morbidity but no death. Full-length genomes were sequenced. Homologic and phylogenetic analyses indicated that these two strains were closely related to Chinese highly pathogenic PRRSV strains. Surprisingly, identical 30 amino acids (aa) deletion in the NSP2-coding region, a presumed high virulence marker, was present in low virulent strain GDQJ. Further comprehensive analysis of GDQJ genome in comparison with Chinese highly pathogenic PRRSV strains revealed multiple genomic variations, distributing in 5' UTR, NSP1b, NSP2, NSP3, NSP5, NSP7, NSP9, NSP10, GP5, and N regions. Data present in this article confirm that the 30 aa deletion in the NSP2-coding region alone is not a reliable genomic indicator for the high virulence of PRRSV strains emerged in China. The genomic variations of GDQJ strain provided the basis for further studies of virulence determinants for PRRSVs.

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“…Zheng et al [45] also suggested co-expression of GP4, GP5, and M proteins of vaccine virus, Ankala. The remaining major structural protein, N protein, is the most abundant and most immunogenic protein in the virion [9] and is an important target for virological detection by PCR [15] and serological detection by ELISA [30], as well as being a phylogenetic marker [44].…”

Section: Introductionmentioning

confidence: 99%

Tracing the genetic history of porcine reproductive and respiratory syndrome viruses derived from the complete ORF 5-7 sequences: a Bayesian coalescent approach

et al. 2012

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Simple and rapid detection of the porcine reproductive and respiratory syndrome virus from pig whole blood using filter paper

Cited by 28 publications

References 17 publications

Molecular characterization of ORFs 2 to 7 of Korean porcine reproductive and respiratory syndrome virus (CA) and its protein expression by recombinant baculoviruses

Molecular characterization of ORFs 2 to 7 of Korean porcine reproductive and respiratory syndrome virus (CA) and its protein expression by recombinant baculoviruses

Genomic analysis of two Chinese strains of porcine reproductive and respiratory syndrome viruses with different virulence

Tracing the genetic history of porcine reproductive and respiratory syndrome viruses derived from the complete ORF 5-7 sequences: a Bayesian coalescent approach

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