Simple and Rapid Colorimetric Biosensors Based on DNA Aptamer and Noncrosslinking Gold Nanoparticle Aggregation

Zhao, Weian; Chiuman, William; Brook, Michael A.; Li, Yingfu

doi:10.1002/cbic.200700014

Cited by 210 publications

(179 citation statements)

References 30 publications

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“…[70][71][72][73][74] This can be achieved through the binding of gold to sulfur-, phosphor-, nitrogen-and oxygen-based ligands, or via non-covalent interaction between capping agents. Thiol-functionalized AuNPs conjugates serve as unique probes for recognizing specific sequences in DNA segments and as building blocks for assembling novel structures and 15 materials.…”

Section: Assays Based On Functionalized Aunpsmentioning

confidence: 99%

“…74,161,177,180 For example, in one study, an adenosine-binding aptamer was hybridized with a short complementary AuNP-conjugated DNA, which was stable and well dispersed in the solution. The aptamer strands underwent structure-switching upon binding adenosine, leading to their release from the AuNPs.…”

Section: Dna Surface Coverage On Aunpsmentioning

confidence: 99%

“…The aptamer strands underwent structure-switching upon binding adenosine, leading to their release from the AuNPs. 74 The ssDNA-conjugated AuNPs now became unstable and aggregated to produce a rapid red-to-purple color change. However, when these AuNPs have high surface coverage (150 strands/AuNP), DNA-modified AuNPs are found highly stable even in the presence of 500 mM MgCl 2 , and the aptamers could not function well under such high divalent metal ion concentrations.…”

Section: Dna Surface Coverage On Aunpsmentioning

confidence: 99%

“…However, when these AuNPs have high surface coverage (150 strands/AuNP), DNA-modified AuNPs are found highly stable even in the presence of 500 mM MgCl 2 , and the aptamers could not function well under such high divalent metal ion concentrations. 74 MCH molecules were therefore added to the solution to decrease DNA surface coverage to 100 DNA strands/AuNP, and successful target detection became possible at this reduced surface coverage. 74 Song and Liu reported colorimetric detection of DNA methylation using DNA-AuNPs in a restriction endonuclease-based assay.…”

Section: Dna Surface Coverage On Aunpsmentioning

confidence: 99%

“…74 Upon binding to their target, aptamer strands underwent a conformational change and dissociated from the probe-modified AuNPs. The unhybridized AuNPs now became unstable at the same salt concentration, causing immediate aggregation with a red to purple color change.…”

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Gold Nanoparticle-Based Colorimetric Sensors for Detection of DNA and Small Molecules

Liang¹

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Section: Assays Based On Functionalized Aunpsmentioning

confidence: 99%

Section: Dna Surface Coverage On Aunpsmentioning

confidence: 99%

Section: Dna Surface Coverage On Aunpsmentioning

confidence: 99%

Section: Dna Surface Coverage On Aunpsmentioning

confidence: 99%

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Gold Nanoparticle-Based Colorimetric Sensors for Detection of DNA and Small Molecules

Liang¹

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Strategy for Use of Smart Routes to Prepare Label‐Free Aptasensors for Bioassay Using Different Techniques

Wei

Dong

2008

Aptamers in Bioanalysis

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Biosensors, which usually need biological molecules as recognition elements, are playing a growing role in various fields, due to their effective selectivity, high sensitivity, and easy performance. Thus, even for only one useful target, it is worth large numbers of designs and improvements to develop detection that is effective enough for a variety of applications. In this way, more and more techniques and novel recognition elements are being brought into bioassays.Aptamers are screened through the systematic evolution of ligands by exponential enrichment (SELEX) process as functional oligonucleotides (Ellington and Szostak, 1990;Robertson and Joyce, 1990;Tuerk and Gold, 1990). Because of their generally impressive selectivity and affinity, they have already attracted much analyst attention and were quick to be used in analytical assays as novel recognition elements. Aptamers exhibit multifarious advantages over traditional recognition elements (Osborne and Ellington, 1997;Jayasena, 1999;Famulok et al., 2000;Tombelli et al., 2005a). For example, they are easy to synthesize in vitro, easy to modify, and flexible to design. These inherent advantages as oligonucleotides not only make target diversification but also improve detection efficiency. They can also help analysts simplify the analytical process and fabricate a sensing platform smartly and conveniently.

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Nucleic Acids as Detection Tools

Lam

Aguirre

2010

The Chemical Biology of Nucleic Acids

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In this chapter, we review some of the popular detection methods that utilize nucleic acids. First, we discuss assorted techniques in which nucleic acid probes are used to detect other nucleic acid sequences, followed by a discussion of functional nucleic acids and their applications as biosensors in conjunction with fluorescent, colorimetric, electrochemical and piezoelectric detection platforms. Detection of nucleic acid targets by nucleic acid probesSince many human diseases are linked to genetic perturbations, identification of specific mutations in patients has become a highly important practice in modern medicine. A great deal of DNA-based diagnostics pertain to identifying subtle differences in sequence content and their associated phenotypes. These differences are often the result of single nucleotide polymorphisms (SNPs) or other sequence alterations (such as point mutations, insertions and deletions). Since SNP is the most frequent form of genetic variation among individuals (roughly one for every 1000 nucleotides), many nucleic acid-based detection technologies are directed at identifying single-nucleotide differences [7,8].Apart from local genetic mutations, structural and distant site changes of DNA sequence are also observed. These large genomic alterations include insertion-deletions, gene copy number variants (CNVs), inversions and translocations, which have been linked to a variety of genetic disorders [9]. For instance, CNVs are characterized by atypical gene content and/or gene deregulation associated with CHARGE syndrome and Parkinson's and Alzheimer's disease [8,10,11]. Currently, there are a plethora of methods for detecting local and structural genetic changes, but here we will only discuss a few major techniques.

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Simple and Rapid Colorimetric Biosensors Based on DNA Aptamer and Noncrosslinking Gold Nanoparticle Aggregation

Cited by 210 publications

References 30 publications

Gold Nanoparticle-Based Colorimetric Sensors for Detection of DNA and Small Molecules

Gold Nanoparticle-Based Colorimetric Sensors for Detection of DNA and Small Molecules

Strategy for Use of Smart Routes to Prepare Label‐Free Aptasensors for Bioassay Using Different Techniques

Nucleic Acids as Detection Tools

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