Simple and quick detection of extended-spectrum <i>β</i>-lactamase and carbapenemase-encoding genes using isothermal nucleic acid amplification techniques
TATSUYA NAKAYAMA,
KEISUKE SOGA
Abstract:The spread of plasmid-mediated antibiotic-resistant bacteria must be controlled; to this end, developing kits for simple and rapid detection in food and clinical settings is desirable. This review describes the detection of antibiotic resistance genes in extended-spectrum β-lactamase (ESBL) -and carbapenemase-producing bacteria. Loop-mediated isothermal amplification (LAMP) , a technique developed in Japan, is a useful diffusion amplification method that does not require equipment like thermal cyclers, and amp… Show more
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