Simple and quantitative analysis of urinary sulfated tauro- and glycodihydroxycholic acids in infant with cholestasis by electrospray ionization mass spectrometry

Shinka, Toshikatsu; Inoue, Yoshito; Ohse, Morimasa; Kuhara, Tomiko

doi:10.1016/j.jchromb.2007.04.010

Cited by 12 publications

(11 citation statements)

References 16 publications

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“…Eleven different bile acids were quantified ( Table 5). As expected from previous results [25,26], the prominent metabolites in urine were sulfated, mainly UDCA and LCA which were also found as glyco-or tauro-conjugates (76% of total urinary bile acids) (Fig. 4B).…”

supporting

confidence: 90%

Bile acid profiling in human biological samples: Comparison of extraction procedures and application to normal and cholestatic patients

Humbert

Maubert

Wolf

et al. 2012

Journal of Chromatography B

143

128

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supporting

confidence: 90%

Bile acid profiling in human biological samples: Comparison of extraction procedures and application to normal and cholestatic patients

Humbert

Maubert

Wolf

et al. 2012

Journal of Chromatography B

143

128

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“…Shinka et al. [18] reported that these ions were stable and can be applied for quantification. Moreover, Li et al.…”

Section: Biological Samplesmentioning

confidence: 99%

Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

2020

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Bile acids are a group of compounds essential for lipid digestion and absorption with a steroid skeleton and a carboxylate side chain usually conjugated to glycine or taurine. Bile acids are regulatory molecules for a number of metabolic processes and can be used as biomarkers of various disorders. Since the middle of the twentieth century, the detection of bile acids has evolved from simple qualitative analysis to accurate quantification in complicated mixtures. Advanced methods are required to characterize and quantify individual bile acids in these mixtures. This article overviews the literature from the last two decades (2000-2020) and focuses on bile acid analysis in various human biological samples. The methods for sample preparation, including the sample treatment of conventional (blood plasma, blood serum, and urine) and unconventional samples (bile, saliva, duodenal/gastric juice, feces, etc.) are shortly discussed. Eventually, the focus is on novel analytical approaches and methods for each particular biological sample, providing an overview of the microcolumn separation techniques, such as high-performance liquid chromatography, gas chromatography, and capillary electrophoresis, used in their analysis. This is followed by a discussion on selected clinical applications.

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“…LC-MS (and MS/MS) has been exploited for bile acid analysis of human and rodent urine ( 43,55,83,86,97,(99)(100)(101)(102), plasma/serum ( 13,55,97,98,(103)(104)(105)(106)(107)(108)(109)(110), bile ( 13,55,111,112 ), cecal content ( 113,114 ), liver ( 55,97,114 ), feces ( 114 ), and more surprisingly, brain ( 65,115 ). However, care must be taken in interpreting LC-MS data, and to postulate a structure based on the mass of an [M-H] Ϫ ion alone can lead to mistaken identifi cations.…”

Section: Lc-msmentioning

confidence: 99%

Bile acids: analysis in biological fluids and tissues

Griffiths

Sjövall

2010

Journal of Lipid Research

164

146

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in lower vertebrates. Metabolism of cholesterol to bile acids/bile alcohols is of major importance for the maintenance and regulation of cholesterol homeostasis. Depending on their structure, bile acids/bile alcohols can be ligands to nuclear receptors with different regulatory functions in animals, including even Caenorhabditis elegans ( 1 ). Bile acids can be formed in some marine microorganisms ( 2 ). These fi ndings have increased interest in methods for specifi c and sensitive analysis of bile acids.Conjugated forms of bile acids/bile alcohols in bile (bile salts) are essential participants in the absorption of lipids and lipid soluble compounds. Studies of this function, bile secretion, enterohepatic circulation, levels in blood, urine, and feces required methods for analysis of bile acids. In 1844, M. von Pettenkofer described his classical color reaction between cholic acid(s) and sugar in conc. sulfuric acid. During the subsequent 100 years, the reaction was modifi ed in numerous ways. This period is described by Sobotka ( 3, 4 ), who pointed out the discrepancies between the poor quality of the analytical methods of his days and the better understanding of bile acid physiology and pathology. He made the following remarks, well worth considering today:"The value of a method lies not only in its specifi city but also in the ease with which interfering factors may be specifi cally removed. To test the usefulness of a method, it is insuffi cient to demonstrate that solutions of the pure substance yield correct results and that known amounts of the substance, when added to biological material, may be completely recovered. It is absolutely necessary to prove that the analytical reaction given by the biological material or extract is due to the substance for which one tests. The unequivocal identifi cation of this substance, in amounts consistent with the analytical data, should alAbstract The formation of bile acids/bile alcohols is of major importance for the maintenance of cholesterol homeostasis. Besides their functions in lipid absorption, bile acids/bile alcohols are regulatory molecules for a number of metabolic processes. Their effects are structure-dependent, and numerous metabolic conversions result in a complex mixture of biologically active and inactive forms. Advanced methods are required to characterize and quantify individual bile acids in these mixtures. A combination of such analyses with analyses of the proteome will be required for a better understanding of mechanisms of action and nature of endogenous ligands. Mass spectrometry is the basic detection technique for effl uents from chromatographic columns. Capillary liquid chromatography-mass spectrometry with electrospray ionization provides the highest sensitivity in metabolome analysis. Classical gas chromatography-mass spectrometry is less sensitive but offers extensive structuredependent fragmentation increasing the specifi city in analyses of isobaric isomers of unconjugated bile acids. Depending on the nature of the bile acid/bile alc...

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Simple and quantitative analysis of urinary sulfated tauro- and glycodihydroxycholic acids in infant with cholestasis by electrospray ionization mass spectrometry

Cited by 12 publications

References 16 publications

Bile acid profiling in human biological samples: Comparison of extraction procedures and application to normal and cholestatic patients

Bile acid profiling in human biological samples: Comparison of extraction procedures and application to normal and cholestatic patients

Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

Bile acids: analysis in biological fluids and tissues

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