Simple and Practical Multigram Synthesis of <scp>d</scp>-Xylonate Using a Recombinant Xylose Dehydrogenase

Sánchez-Moreno, Israel; Benito-Arenas, Raúl; Montero-Calle, Pilar; Hermida, Carmen; García‐Junceda, Eduardo; Fernández-Mayoralas, Alfonso

doi:10.1021/acsomega.9b01090

Cited by 3 publications

(4 citation statements)

References 27 publications

(54 reference statements)

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“…Xylose reductase activity was determined as described elsewhere [26] with a final sample composition of 100 mM potassium phosphate (pH 6.0) reaction buffer, 0.2 mM NADPH, and 200 mM xylose. Xylose reductase activity was determined as described elsewhere [69] with a final sample composition of 50 mM sodium phosphate (pH 8) reaction buffer, 2 mM NAD, and 200 mM xylose.…”

Section: Enzymatic Activity Assaysmentioning

confidence: 99%

Nitrogen-controlled Valorization of Xylose-derived Compounds by Metabolically Engineered Corynebacterium glutamicum

Schwardmann¹,

Rieks²,

Wendisch³

2023

Synthetic Biology and Engineering

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The implementation of bioprocesses in an economically feasible and industrial competitive manner requires the optimal allocation of resources for a balanced distribution between biomass formation and product synthesis. The decoupling of growth and production in two-stage bioprocesses, aiming to ensure sufficient growth before the onset of production, is particularly relevant when target products inhibit growth. In order to avoid expensive inducer molecules, continuing process monitoring, elaborate individual process optimization, and strain engineering, we developed and applied nitrogen deprivation-induced expression of genes for product biosynthesis. Two native nitrogen deprivation-inducible promoters were identified and shown to function for dynamic growth-decoupled gene expression or CRISPRi-mediated gene knockdown in C. glutamicum with superior induction factors than the standard IPTG-inducible Ptrc promoter. Valorization of xylose to produce either the sugar acid xylonic acid or the sugar alcohol xylitol from xylose as sole source of carbon and energy was demonstrated. Competitive titers of up to 34 g•L −1 xylonate and 13 g•L −1 xylitol were achieved in two-stage processes. We discussed that the transfer to bioprocesses with C. glutamicum using carbon sources other than xylose appears straightforward in particular regarding production of growth-inhibitory compounds by their growthdecoupled fermentative production.

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Section: Enzymatic Activity Assaysmentioning

confidence: 99%

Nitrogen-controlled Valorization of Xylose-derived Compounds by Metabolically Engineered Corynebacterium glutamicum

Schwardmann¹,

Rieks²,

Wendisch³

2023

Synthetic Biology and Engineering

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“…48 A different scheme with xylose dehydrogenase and its cofactor – NAD + – regeneration with alcohol dehydrogenase using acetaldehyde as a sacrificial substrate is reported as well. 16 Authors do not report TTN E for xylose dehydrogenase, but that can be estimated from the information available to be ∼8500.…”

Section: Resultsmentioning

confidence: 99%

“…Nevertheless, if coupled with some NAD(P) + re-oxidation process, these enzymes can be used for the production of aldonic acids. 16 The regeneration of NAD(P) + can be achieved by various means, of which the most important are – enzymatic coupling with some sacrificial substrate, electrochemical and photochemical oxidations. The enzymatic systems of NAD(P) + regeneration exploit various enzymes such as glutamate dehydrogenase, l -lactate dehydrogenase, or NADH oxidase with their respective sacrificial substrates ketoglutarate and ammonium ions, pyruvate, oxygen.…”

Section: Introductionmentioning

confidence: 99%

Efficient Bi-enzymatic synthesis of aldonic acids

et al. 2022

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“…In addition to whole-cell biocatalysis, the use of isolated enzymes to construct an enzymatic cascade of XYD and alcohol dehydrogenase (ADH) under alkaline conditions was established (Figure 17B). 225 XYD catalyzes conversion of xylose to xylonolactone whereas ADH catalyzes reduction of aldehyde to alcohol with simultaneous regeneration of NADH to NAD + to be used in the XYD reaction. The reactions were maintained at pH 8.0 which pushed the equilibrium toward xylonate formation because the produced lactone can undergo spontaneous hydrolysis at alkaline pH.…”

Section: Production Of Lignocellulosic Biomass-derived Productsmentioning

confidence: 99%

Enzymes, In Vivo Biocatalysis, and Metabolic Engineering for Enabling a Circular Economy and Sustainability

et al. 2021

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Since the industrial revolution, the rapid growth and development of global industries have depended largely upon the utilization of coal-derived chemicals, and more recently, the utilization of petroleum-based chemicals. These developments have followed a linear economy model (produce, consume, and dispose). As the world is facing a serious threat from the climate change crisis, a more sustainable solution for manufacturing, i.e., circular economy in which waste from the same or different industries can be used as feedstocks or resources for production offers an attractive industrial/business model. In nature, biological systems, i.e., microorganisms routinely use their enzymes and metabolic pathways to convert organic and inorganic wastes to synthesize biochemicals and energy required for their growth. Therefore, an understanding of how selected enzymes convert biobased feedstocks into special (bio)chemicals serves as an important basis from which to build on for applications in biocatalysis, metabolic engineering, and synthetic biology to enable biobased processes that are greener and cleaner for the environment. This review article highlights the current state of knowledge regarding the enzymatic reactions used in converting biobased wastes (lignocellulosic biomass, sugar, phenolic acid, triglyceride, fatty acid, and glycerol) and greenhouse gases (CO 2 and CH 4 ) into value-added products and discusses the current progress made in their metabolic engineering. The commercial aspects and life cycle assessment of products from enzymatic and metabolic engineering are also discussed. Continued development in the field of metabolic engineering would offer diversified solutions which are sustainable and renewable for manufacturing valuable chemicals.

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Simple and Practical Multigram Synthesis of d-Xylonate Using a Recombinant Xylose Dehydrogenase

Cited by 3 publications

References 27 publications

Nitrogen-controlled Valorization of Xylose-derived Compounds by Metabolically Engineered Corynebacterium glutamicum

Nitrogen-controlled Valorization of Xylose-derived Compounds by Metabolically Engineered Corynebacterium glutamicum

Efficient Bi-enzymatic synthesis of aldonic acids

Enzymes, In Vivo Biocatalysis, and Metabolic Engineering for Enabling a Circular Economy and Sustainability

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