Simple and Micro Determination of Manganese in Serum by Graphite Furnace Atomic Absorption Spectrometry

Abstract

A method for the determination of low level manganese in serum by graphite furnace atomic absorption spectrometry was established. Serum was diluted two-fold with Triton X-100 without deproteinization. The final concentration of the detergent was 0.2%. Manganese was measured twice by 20 µl injection of sample solution by a standard addition method. For the serum manganese of 0.52 ng/ml, the relative standard deviation was below 5%. Special precautions for sampling of serum and some sources of contamination are also described.

Direct Determination of Manganese in Biological Materials Using Electrothermal Atomization Atomic Absorption Spectrometry with a Molybdenum Tube Atomizer

“…[16][17][18] For removing this interference, a matrix modifier, a matrixmatched calibration graph, organic solvent extraction and ion exchange separation methods have been combined. 1,9,10,12,14,[17][18][19][20] This paper reports on a direct determination of manganese in biological materials by ETA-AAS with a molybdenum tube atomizer and thiourea used as a …”