Simple and efficient UPLC-ESI-MS/MS method for multi-residue analysis of 14 coccidiostatic agents in poultry liver and muscle tissues

Rydchuk, Mariana; Plotytsia, Serhiy; Zasadna, Zvenyslava; Yanovych, D.V.

doi:10.1016/j.foodcont.2023.109815

Cited by 2 publications

(2 citation statements)

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“…Since their usage is not permitted for laying hens, coccidiostats could not be detected in eggs. Compared to the LOD achieved by the organic solvent extraction method (2 µg/kg for DIZ and DNC; 1.2, 1.6, and 3.2 µg/kg for TOZ, TZS, TZSO) 7 , this approach is a significant improvement. Taking into account the LOQ, the medium and high points within the linear range, as well as the MRL, the spiking levels were established at 1, 10, 20, 100, and 200 µg/kg with six parallel measurements.…”

Section: Linear Range and Lodsmentioning

confidence: 94%

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Simultaneous Analysis of Nicarbazin, Diclazuril, Toltrazuril, and Its Two Metabolites in Chicken Muscle and Eggs by In-Syringe Dispersive Solid-Phase Filter Clean-Up Followed by Liquid Chromatography–Tandem Mass Spectrometry

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Pan,

Cai

et al. 2024

Foods

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Nicarbazin (NICA) and triazine anticoccidial drugs (diclazuril (DIZ) and toltrazuril (TOZ)) are the primary strategy for preventing and treating coccidiosis. To prevent the development of drug resistance and mitigate the potential chronic toxicity to humans resulting from prolonged exposure, a liquid chromatography–tandem mass spectrometry method with high reliability and sensitivity was developed to determine NICA, DIZ, TOZ, and its two metabolites in chicken muscle and eggs. Upon establishing the extraction conditions involving 10 mL of acetonitrile and 10 min of sonication, in-syringe dispersive solid-phase extraction with silica was performed in combination with n-hexane clean-up. The selection of isotope peaks of precursor ions and low-mass range scanning allowed the two transitions for the quantification of all compounds. The limits of detection for DIZ and NICA were both 0.1 μg/kg, and for TOZ and metabolites, they were 0.3 μg/kg; the limits of quantitation were 0.3 and 1 μg/kg, respectively. The linear range was 0.25–50 ng/mL with a correlation coefficient r > 0.999. The average recoveries at three spiking levels in muscle and eggs were 90.1–105.2% and 94.0–103.7% with the relative standard deviations of 3.0–8.1% and 3.1–14.4%, respectively. The precision, accuracy, and stability were evaluated by three quality control samples.

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Section: Linear Range and Lodsmentioning

confidence: 94%

“…NICA refers to the combination of 4,4′-dinitrocarbanilide (DNC) and 2-hydroxy-4,6-dimethylpyrimidine (HDP) in a 1:1 molar ratio. DNC is commonly employed as the marker for the residue of NICA in animal food [ 7 ]. The structures of these drugs are shown in Figure 1 .…”

Section: Introductionmentioning

confidence: 99%