Similarity assessment of charge variants for bevacizumab biosimilar formulations using imaged capillary isoelectric focusing

Comparative stability study and aggregate analysis of Bevacizumab marketed formulations using advanced analytical techniques

“…4 G, main and acidic species of Bio-1 and Bio-2 were found to be similar to that of Avt. However prominent differences were observed in basic species of biosimilar formulations, which can be due to the presence of C-terminal lysine residue [ 15 ]. Further changes in HMW species and NGHC content observed between the formulations were shown in Fig.…”

“…For charge heterogeneity analysis of Bio-1 and Bio-2 in comparison to Avt, the reported iCIEF method along with the sample preparation step was used [ 15 ]. Using the developed method, the apparent isoelectric point ( pI) of the formulations as well as the percentage of acidic, neutral and basic species was calculated.…”