Similarities and differences in the transcriptional regulation of the leptin gene promoter in gastric and adipose cells

̈ot, Hélène Goı; Laigneau, Jean–Pierre; Devaud, Hélène; Sobhani, Iradj; Bado, André

doi:10.1016/j.febslet.2005.02.031

Cited by 10 publications

(7 citation statements)

References 67 publications

(71 reference statements)

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“…Indeed, colocalization of CCK1R and leptin was demonstrated in canine chief cells, and activation of CCK1R was shown to enhance leptin protein amounts as well as to dose-dependently stimulate leptin secretion (532). In agreement with this study, CCK was recently reported to activate the leptin gene promoter in gastric cells (179). These data argue for a role of CCK1R in the physiological functions of gastric leptin.…”

Section: Stomachsupporting

confidence: 77%

Cholecystokinin and Gastrin Receptors

Dufresne

Seva²,

Fourmy³

2006

Physiological Reviews

414

418

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Cholecystokinin and gastrin receptors (CCK1R and CCK2R) are G protein-coupled receptors that have been the subject of intensive research in the last 10 years with corresponding advances in the understanding of their functioning and physiology. In this review, we first describe general properties of the receptors, such as the different signaling pathways used to exert short- and long-term effects and the structural data that explain their binding properties, activation, and regulation. We then focus on peripheral cholecystokinin receptors by describing their tissue distribution and physiological actions. Finally, pathophysiological peripheral actions of cholecystokinin receptors and their relevance in clinical disorders are reviewed.

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Section: Stomachsupporting

confidence: 77%

Cholecystokinin and Gastrin Receptors

Dufresne

Seva²,

Fourmy³

2006

Physiological Reviews

414

418

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“…Notably, the lack of recovery with refeeding in gastric leptin levels (considering the rise in the mRNA levels) would reflect its secretion in response to food intake, according to its role as a satiety signal [8,11,44]. Curiously, gastric leptin levels were positively correlated with circulating insulin levels (r=0.363, p<0.05) and this may suggest the possibility that gastric leptin levels may depend, at least in part, on circulating insulin levels; along these lines, not only has adipose leptin expression been clearly shown to be induced by insulin [45][46][47], but it also happens for gastric leptin as shown in human gastric cultured cells [48].…”

Section: Discussionmentioning

confidence: 84%

Induction of NPY/AgRP Orexigenic Peptide Expression in Rat Hypothalamus is an early Event in Fasting: Relationship with Circulating Leptin, Insulin and Glucose

Palou

Sánchez

Rodríguez

et al. 2009

Cell Physiol Biochem

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Hypothalamus is crucial in the control of energy intake and expenditure in mammals, presenting two interconnected populations of neurons producing orexigenic NPY/AgRP (neuropeptide Y; agouti related peptide) and anorexigenic POMC/CART (pro-opiomelanocortin; cocaine and amphetamine regulated transcript) neuropeptides. We aimed to shed more light on the response and sensitivity in the production of these neuropeptides to face nutritional changes, particularly food deprivation, and on the signals that regulate them. Male Wistar rats were fasted for 0, 4, 8 and 24h and refed for 3h after 8h fasting. mRNA levels of gastric and adipose tissue (retroperitoneal, mesenteric and inguinal) leptin, and of hypothalamic NPY, AgRP, POMC, CART, leptin receptor, SOCS3 (suppressor of cytokine signaling 3) and insulin receptor were analyzed. Gastric and circulating leptin, and circulating insulin, glucose and ghrelin were also determined. The only neuropeptide mRNAs that responded (increasing) to the short-term periods of fasting used were those of NPY (transiently) and AgRP, and these changes were accompanied by an increase in leptin receptor mRNA levels and by a decrease in adipose and gastric leptin expression and in the circulating levels of leptin, insulin and glucose, but without changes in circulating ghrelin. The elevation in AgRP and leptin receptor mRNA levels and the drop in circulating leptin were not reverted with refeeding. It is suggested that the induction of expression of the orexigenic molecules in NPY/AgRP neurons is an early event upon fasting, related with changes in leptin, insulin and glucose levels, but with the role of leptin signaling in particular.

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“…Small amounts of leptin have been reported in brain, pituitary, trophoblast, stomach, mammary epithelial cells, liver, chondrocytes and muscle, 4 , 12 - 14 but the physiological importance of this low level expression, if any, has not been determined.…”

Section: Transcriptional Regulation Of Lep Gene Expressionmentioning

confidence: 99%

New insights into adipocyte-specific leptin gene expression

Wrann

Rosen

2012

Adipocyte

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The adipocyte-derived hormone leptin is a critical regulator of many physiological functions, ranging from satiety to immunity. Surprisingly, very little is known about the transcriptional pathways that regulate adipocyte-specific expression of leptin. In a recent published study, we pursued a strategy integrating BAC transgenic reporter mice, in vitro reporter assays, and chromatin state mapping to locate an adipocyte-specific cis-element upstream of the LEP gene in human fat cells. Quantitative proteomics (stable isotope labeling by amino acids in cell culture, SILAC) with affinity enrichment of protein-DNA complexes identified the transcription factor FOSL2 as a specific binder to the identified region. We confirmed that FOSL2 is an important regulator of LEP gene expression in vitro and in vivo using cell culture models and genetic mouse models. In this commentary, we discuss the transcriptional regulation of LEP gene expression, our strategy to identify an adipocyte-specific cis-regulatory element and the transcription factor(s) responsible for LEP gene expression. We also discuss our data on FOSL2 and leptin levels in physiology and pathophysiology. We speculate on unanswered questions and future directions.

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Similarities and differences in the transcriptional regulation of the leptin gene promoter in gastric and adipose cells

Cited by 10 publications

References 67 publications

Cholecystokinin and Gastrin Receptors

Cholecystokinin and Gastrin Receptors

Induction of NPY/AgRP Orexigenic Peptide Expression in Rat Hypothalamus is an early Event in Fasting: Relationship with Circulating Leptin, Insulin and Glucose

New insights into adipocyte-specific leptin gene expression

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