Similar sponge‐associated bacteria can be acquired via both vertical and horizontal transmission

Sipkema, Detmer; Caralt, Sònia de; Morillo, José A.; Al‐Soud, Waleed Abu; Sørensen, Søren J.; Smidt, Hauke; Uriz, María Jesús

doi:10.1111/1462-2920.12827

Cited by 71 publications

(69 citation statements)

References 72 publications

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“…Indeed, the symbiosis may be propagated by two mechanisms: vertical transmission from maternal tissues to the progeny, as evident in our study, and horizontal transmission from the environment, as suggested by the presence of free calcibacteria in seawater. Redundant mechanisms for assuring a relevant biological function are frequent in nature19 and two acquisition modes of symbiotic microbes have also been reported for other sponges20.…”

Section: Discussionmentioning

confidence: 99%

Endosymbiotic calcifying bacteria across sponge species and oceans

Garate

Sureda

Agell

et al. 2017

Sci Rep

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From an evolutionary point of view, sponges are ideal targets to study marine symbioses as they are the most ancient living metazoans and harbour highly diverse microbial communities. A recently discovered association between the sponge Hemimycale columella and an intracellular bacterium that generates large amounts of calcite spherules has prompted speculation on the possible role of intracellular bacteria in the evolution of the skeleton in early animals. To gain insight into this purportedly ancestral symbiosis, we investigated the presence of symbiotic bacteria in Mediterranean and Caribbean sponges. We found four new calcibacteria OTUs belonging to the SAR116 in two orders (Poecilosclerida and Clionaida) and three families of Demospongiae, two additional OTUs in cnidarians and one more in seawater (at 98.5% similarity). Using a calcibacteria targeted probe and CARD-FISH, we also found calcibacteria in Spirophorida and Suberitida and proved that the calcifying bacteria accumulated at the sponge periphery, forming a skeletal cortex, analogous to that of siliceous microscleres in other demosponges. Bacteria-mediated skeletonization is spread in a range of phylogenetically distant species and thus the purported implication of bacteria in skeleton formation and evolution of early animals gains relevance.

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Section: Discussionmentioning

confidence: 99%

Endosymbiotic calcifying bacteria across sponge species and oceans

Garate

Sureda

Agell

et al. 2017

Sci Rep

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“…; Sipkema et al . )]. For the latter mode of symbiont acquisition, a cellular recognition is required that discriminates symbiont from nonsymbiotiv microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Expression of eukaryotic‐like protein in the microbiome of sponges

et al. 2017

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Eukaryotic-like proteins (ELPs) are classes of proteins that are found in prokaryotes, but have a likely evolutionary origin in eukaryotes. ELPs have been postulated to mediate host-microbiome interactions. Recent work has discovered that prokaryotic symbionts of sponges contain abundant and diverse genes for ELPs, which could modulate interactions with their filter-feeding and phagocytic host. However, the extent to which these ELP genes are actually used and expressed by the symbionts is poorly understood. Here, we use metatranscriptomics to investigate ELP expression in the microbiomes of three different sponges (Cymbastella concentrica, Scopalina sp. and Tedania anhelens). We developed a workflow with optimized rRNA removal and in silico subtraction of host sequences to obtain a reliable symbiont metatranscriptome. This showed that between 1.3% and 2.3% of all symbiont transcripts contain genes for ELPs. Two classes of ELPs (cadherin and tetratricopeptide repeats) were abundantly expressed in the C. concentrica and Scopalina sp. microbiomes, while ankyrin repeat ELPs were predominant in the T. anhelens metatranscriptome. Comparison with transcripts that do not encode ELPs indicated a constitutive expression of ELPs across a range of bacterial and archaeal symbionts. Expressed ELPs also contained domains involved in protein secretion and/or were co-expressed with proteins involved in extracellular transport. This suggests these ELPs are likely exported, which could allow for direct interaction with the sponge. Our study shows that ELP genes in sponge symbionts represent actively expressed functions that could mediate molecular interaction between symbiosis partners.

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“…If hosts control endophytic colonization, fitness benefits conferred to the host as a result of microbial NP production may, through long periods of hostsymbiont coevolution, lead to enriched plant colonization by NP-producing symbionts. Although both vertical (from parents to offspring) and horizontal (inoculation from environmental populations of bacteria) transmission are possible in sponges and plants [89,101], vertically-transmitted microbes, which are likely to be especially co-adapted to their hosts, may be most fruitful as targets for novel NPs.…”

Section: Roles Of Nps In Biotic Interactionsmentioning

confidence: 99%

Leveraging ecological theory to guide natural product discovery

Smanski

Schlatter

Kinkel

2016

Journal of Industrial Microbiology and Biotechnology

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Technological improvements have accelerated natural product (NP) discovery and engineering to the point that systematic genome mining for new molecules is on the horizon. NP biosynthetic potential is not equally distributed across organisms, environments, or microbial life histories, but instead is enriched in a number of prolific clades. Also, NPs are not equally abundant in nature; some are quite common and others markedly rare. Armed with this knowledge, random 'fishing expeditions' for new NPs are increasingly harder to justify. Understanding the ecological and evolutionary pressures that drive the non-uniform distribution of NP biosynthesis provides a rational framework for the targeted isolation of strains enriched in new NP potential. Additionally, ecological theory leads to testable hypotheses regarding the roles of NPs in shaping ecosystems. Here we review several recent strain prioritization practices and discuss the ecological and evolutionary underpinnings for each. Finally, we offer perspectives on leveraging microbial ecology and evolutionary biology for future NP discovery.

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Similar sponge‐associated bacteria can be acquired via both vertical and horizontal transmission

Cited by 71 publications

References 72 publications

Endosymbiotic calcifying bacteria across sponge species and oceans

Endosymbiotic calcifying bacteria across sponge species and oceans

Expression of eukaryotic‐like protein in the microbiome of sponges

Leveraging ecological theory to guide natural product discovery

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