Similar Seed Composition Phenotypes Are Observed From CRISPR-Generated In-Frame and Knockout Alleles of a Soybean KASI Ortholog

Virdi, Kamaldeep S.; Spencer, Madison; Stec, Adrian O.; Xiong, Yer; Merry, Ryan; Muehlbauer, Gary J.; Stupar, Robert M.

doi:10.3389/fpls.2020.01005

Cited by 12 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A previous study has linked null alleles for a soybean KASI gene with a pleiotropic phenotype, including a reduction in oil content (Virdi et al., 2020). A mutation in KASIII has also been linked to reduced saturated fatty acid content in soybean (Cardinal et al., 2014).…”

Section: Resultsmentioning

confidence: 99%

Diversity of fatty acid biosynthesis genes across the soybean pangenome

et al. 2023

View full text Add to dashboard Cite

Soybean (Glycine max) is a major crop that contributes more than half of global oilseed production. Much research has been directed towards improvement of the fatty acid profile of soybean seeds through marker assisted breeding. Recently published soybean pangenomes, based on thousands of soybean lines, provide an opportunity to identify new alleles that may be involved in fatty acid biosynthesis. In this study, we identify fatty acid biosynthesis genes in soybean pangenomes based on sequence identity with known genes and examine their sequence diversity across diverse soybean collections. We find three possible instances of a gene missing in wild soybean, including FAD8 and FAD2-2D, which may be involved in oleic and linoleic acid desaturation, respectively, although we recommend follow-up research to verify the absence of these genes. More than half of the 53 fatty acid biosynthesis genes identified contained missense variants, including one linked with a previously identified QTL for oil quality. These variants were present in multiple studies based on either short read mappings or alignment of reference grade genomes. Missense variants were found in previously characterized genes including FAD2-1A and FAD2-1B, both of which are involved in desaturation of oleic acid, as well as uncharacterized candidate fatty acid biosynthesis genes. We find that the frequency of missense alleles in fatty acid biosynthesis genes has been reduced significantly more than the global average frequency of missense mutations during domestication, and missense variation in some genes is near absent in modern cultivars. This could be due to the selection for fatty acid profiles in seed, though future work should be conducted towards understanding the phenotypic impacts of these variants.

show abstract

Section: Resultsmentioning

confidence: 99%

Diversity of fatty acid biosynthesis genes across the soybean pangenome

et al. 2023

View full text Add to dashboard Cite

show abstract

“…In soybean, a disarmed hyper-virulent strain of A. rhizogenes lacking the root-inducing T-DNA region on the Ri-plasmid was developed [139]. Disarmed A. rhizogenes containing GE binary vectors have been used for the generation of several mutated soybean plants [140,141,144,145] (Table S1).…”

Section: Agrobacterium Rhizogenes-mediated Transformationmentioning

confidence: 99%

Opportunities and Challenges of In Vitro Tissue Culture Systems in the Era of Crop Genome Editing

Bekalu

Panting

Holme

et al. 2023

IJMS

View full text Add to dashboard Cite

Currently, the development of genome editing (GE) tools has provided a wide platform for targeted modification of plant genomes. However, the lack of versatile DNA delivery systems for a large variety of crop species has been the main bottleneck for improving crops with beneficial traits. Currently, the generation of plants with heritable mutations induced by GE tools mostly goes through tissue culture. Unfortunately, current tissue culture systems restrict successful results to only a limited number of plant species and genotypes. In order to release the full potential of the GE tools, procedures need to be species and genotype independent. This review provides an in-depth summary and insights into the various in vitro tissue culture systems used for GE in the economically important crops barley, wheat, rice, sorghum, soybean, maize, potatoes, cassava, and millet and uncovers new opportunities and challenges of already-established tissue culture platforms for GE in the crops.

show abstract

“…β-ketoacyl-[acyl carrier protein] synthase 1 (KASI) plays an important role as a fatty acid biosynthetic enzyme and is involved in the conversion of sucrose to fatty acids during soybean seed development ( Dobbels et al 2017 ). Site-directed mutagenesis of the KASI gene by the CRISPR/Cas9 system was performed using the Agrobacterium -mediated transformation platform ( Virdi et al 2020 ). Mature seeds that had homozygous in-frame mutant alleles at the targeted locus exhibited a wrinkled and cracked seed-coat phenotype, but showed an increase in sucrose content from 5.97% to 11.50% as well as a decrease in oil content from 18.76% to 5.63% compared with wild-type seeds ( Virdi et al 2020 ).…”

Section: Improvement Of Seed Traits By Crispr/cas9 Systemmentioning

confidence: 99%

Recent advances in the improvement of soybean seed traits by genome editing

Sim,

Kuwabara,

Sugano

et al. 2023

Plant Biotechnology

View full text Add to dashboard Cite

Genetic improvement of soybean seed traits is important for developing new varieties that meet the demand for soybean as a food, forage crop, and industrial products. A large number of soybean genome sequences are currently publicly available. This genome sequence information provides a significant opportunity to design genomic approaches to improve soybean traits. Genome editing represents a major advancement in biotechnology. The production of soybean mutants through genome editing is commonly achieved with either an Agrobacterium -mediated or biolistic transformation platform, which have been optimized for various soybean genotypes. Currently, the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated endonuclease 9 (Cas9) system, which represents a major advance in genome editing, is used to improve soybean traits, such as fatty acid composition, protein content and composition, flavor, digestibility, size, and seed-coat color. In this review, we summarize the recent advances in the improvement of soybean seed traits through genome editing. We also discuss the characteristics of genome editing using the CRISPR/Cas9 system with transformation platforms.

show abstract

Similar Seed Composition Phenotypes Are Observed From CRISPR-Generated In-Frame and Knockout Alleles of a Soybean KASI Ortholog

Cited by 12 publications

References 18 publications

Diversity of fatty acid biosynthesis genes across the soybean pangenome

Diversity of fatty acid biosynthesis genes across the soybean pangenome

Opportunities and Challenges of In Vitro Tissue Culture Systems in the Era of Crop Genome Editing

Recent advances in the improvement of soybean seed traits by genome editing

Contact Info

Product

Resources

About