Similar proteomic profiles of human mesenchymal stromal cells from different donors

Lazzarotto-silva, Carolina; Binato, Renata; Rocher, Bárbara Du; Costa, Júlia Assunção Costa E; Pizzatti, Luciana; Bouzas, Luis Fernando; Abdelhay, Eliana

doi:10.1080/14653240902783268

Cited by 12 publications

(7 citation statements)

References 32 publications

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“…Recent studies have characterized the cell surface proteome of human MSC which will allow better characterisation of sub-populations of stem cells derived from different tissues [30]. Another study showed that human bone marrow MSC derived from different donors had very similar proteomic expression profiles [31]. Proteomic analysis has also been used to study changes in protein expression occurring as a result of differentiation of human MSC towards the osteogenic lineage [32].…”

Section: Discussionmentioning

confidence: 99%

Aging Effect on Neurotrophic Activity of Human Mesenchymal Stem Cells

et al. 2012

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Clinical efficacy of stem cells for nerve repair is likely to be influenced by issues including donor age and in vitro expansion time. We isolated human mesenchymal stem cells (MSC) from bone marrow of young (16–18 years) and old (67–75 years) donors and analyzed their capacity to differentiate and promote neurite outgrowth from dorsal root ganglia (DRG) neurons. Treatment of MSC with growth factors (forskolin, basic fibroblast growth factor, platelet derived growth factor-AA and glial growth factor-2) induced protein expression of the glial cell marker S100 in cultures from young but not old donors. MSC expressed various neurotrophic factor mRNA transcripts. Growth factor treatment enhanced the levels of BDNF and VEGF transcripts with corresponding increases in protein release in both donor cell groups. MSC in co-culture with DRG neurons significantly enhanced total neurite length which, in the case of young but not old donors, was further potentiated by treatment of the MSC with the growth factors. Stem cells from young donors maintained their proliferation rate over a time course of 9 weeks whereas those from the old donors showed increased population doubling times. MSC from young donors, differentiated with growth factors after long-term culture, maintained their ability to enhance neurite outgrowth of DRG. Therefore, MSC isolated from young donors are likely to be a favourable cell source for nerve repair.

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Section: Discussionmentioning

confidence: 99%

Aging Effect on Neurotrophic Activity of Human Mesenchymal Stem Cells

et al. 2012

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“…It has also been used to analyze the The protein expression of the suspected protein, VCP. *p<0.05 compared to the other groups differences in protein expression for stem cells from various sources, as well as those with different differentiation status and donors [22,23]. In addition, 2-DGE can be used to identify the biomarkers for disease diagnosis in human patients [24].…”

Section: Discussionmentioning

confidence: 99%

Human endothelial cell response to polyurethane–gold nanocomposites

Lin²,

Hsu

2012

Gold Bull

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Gold (Au) is long considered as a biocompatible metal, and gold nanoparticles (AuNPs~5 nm) were recently reported to scavenge free radicals. The effect of Au embedded in a polymeric material is less investigated compared with that of silver. In this study, nanocomposites from polyurethane (PU) and 43.5 or 174 ppm of AuNPs were prepared from a waterborne process. The response of endothelial cells (ECs) to the PU-Au nanocomposites was investigated in vitro and in vivo. ECs on PU-Au nanocomposites showed lamellipodia formation and better cell proliferation. The activation of proteins in ECs grown on PU-Au nanocomposites was analyzed by two-dimensional gel electrophoresis and confirmed by Western blot. The new protein identified through this procedure was valosin-containing protein (VCP), which is known to have immunomodulating effect. VCP was upregulated by PU-Au 43.5 ppm and PU-Au 174 ppm, but more in PU-Au 43.5 ppm. This suggested that the dispersion of AuNPs in the polymer matrix may be more important than the loading amount. PU-Au catheters implanted in rat blood vessels showed less foreign body reaction and more extensive EC coverage than the control PU catheters. The good in vivo biocompatibility of PU-Au may be associated with the anti-inflammatory effect of PU-Au. Based on this study, AuNPs may serve as an antioxidant additive for biomedical polymers.

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“…Protein extracts were resuspended in buffer containing 6 M Urea, 2 M Thiourea, 15 mM DTT, 2% (w/v) ASB14, 0.5% IPG buffer (pH 3–10) and bromophenol blue traces and 2-D gel electrophoresis were performed according to Lazzarotto et al . 39 .…”

Section: Methodsmentioning

confidence: 99%

Upregulation of tropomyosin alpha-4 chain in patients’ saliva with oral squamous cell carcinoma as demonstrated by Phage display

Faria

Carneiro

Binato

et al. 2019

Sci Rep

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Patients with oral squamous cell carcinoma (OSCC) present significant alterations in their saliva proteome. We have used the shotgun Phage Display (PD) technology to identify candidate proteins that were upregulated in saliva of OSCC by selecting ligands to salivary proteins from a single-chain variable fragment (scFv) PD combinatorial library. After two selection cycles, the highly reactive clone scFv-D09 was able to distinguish saliva of OSCC patients from healthy subjects by enzyme-linked immunosorbent assay (ELISA) with sensitivity and specificity of 96.67%. Additionally, the scFv-D09 clone presented a positive immunostaining for invasive malignant epithelial cells in the connective tissue, keratin pearls in the OSCC, and ducts of salivary glands. We have further identified the target protein as the tropomyosin alpha-4 chain (TPM4) by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry, and its binding to the scFV-D09 was demonstrated by bioinformatics. Briefly, we have identified TPM4 as upregulated salivary protein in patients with OSCC, which plays a central role in stabilizing cytoskeleton actin filaments, probably linked with tumor tissue remodeling. Long-term longitudinal studies are needed to validate TPM4 as a potential marker of a malignant process.

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Similar proteomic profiles of human mesenchymal stromal cells from different donors

Cited by 12 publications

References 32 publications

Aging Effect on Neurotrophic Activity of Human Mesenchymal Stem Cells

Aging Effect on Neurotrophic Activity of Human Mesenchymal Stem Cells

Human endothelial cell response to polyurethane–gold nanocomposites

Upregulation of tropomyosin alpha-4 chain in patients’ saliva with oral squamous cell carcinoma as demonstrated by Phage display

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