Similar Metabolic, Innate Immunity, and Adipokine Profiles in Adult and Pediatric Sepsis Versus Systemic Inflammatory Response Syndrome—A Pilot Study

Tavladaki, Theonymfi; Spanaki, Anna Maria; Dimitriou, Helen; Kondili, Efmorfia; Choulaki, Christianna; Georgopoulos, Dimitris; Briassoulis, George

doi:10.1097/pcc.0000000000001300

Cited by 32 publications

(21 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Relative to other stages, mature enamel contained more V-Type ATPase H+ transporting subunits including B2, E1, and H, and also expressed the metabolic enzymes pyruvate kinase and phosphoglycerate kinase. In erupted enamel, we detected higher quantities of lipocalin 2 (LCN2), resistin (RETN), and peptidoglycan-recognition protein 1 (PGLYRP1) (Figure 5B), all of which were previously identified in saliva and are known to be involved in innate and adaptive immunity (Mamali et al, 2012; Aqrawi et al, 2017; Nylund et al, 2017; Tavladaki et al, 2017; Xiao et al, 2017; Dziarski and Gupta, 2018; Prims et al, 2019). Proteins we located at high abundance at the DEJ include desmoglein-1 (DSG1), collagen alpha-1(II) chain (COL2A1), and fibronectin (FN), consistent with recently published results obtained through a laser-based microsampling and tandem MS technique (Jágr et al, 2019).…”

Section: Resultsmentioning

confidence: 89%

“…The confirmation of the presence of DEJ proteins located in this tissue by microsampling previously (Jágr et al, 2019), and the presence of salivary and immune function proteins almost exclusively within erupted enamel (Figure 5B), encourages further study. In particular the location of proteins LCN2, RETN, and PGLYRP1 in erupted enamel raises the question of whether these are being sampled from the enamel pellicle, or may be infiltrating into mature erupted enamel as a normal part of posteruptive enamel transformation (Mamali et al, 2012; Aqrawi et al, 2017; Nylund et al, 2017; Tavladaki et al, 2017; Xiao et al, 2017; Dziarski and Gupta, 2018; Prims et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Mapping the Tooth Enamel Proteome and Amelogenin Phosphorylation Onto Mineralizing Porcine Tooth Crowns

Green¹,

Schulte²,

Lee

et al. 2019

Front. Physiol.

View full text Add to dashboard Cite

Tooth enamel forms in an ephemeral protein matrix where changes in protein abundance, composition and posttranslational modifications are critical to achieve healthy enamel properties. Amelogenin (AMELX) with its splice variants is the most abundant enamel matrix protein, with only one known phosphorylation site at serine 16 shown in vitro to be critical for regulating mineralization. The phosphorylated form of AMELX stabilizes amorphous calcium phosphate, while crystalline hydroxyapatite forms in the presence of the unphosphorylated protein. While AMELX regulates mineral transitions over space and time, it is unknown whether and when un-phosphorylated amelogenin occurs during enamel mineralization. This study aims to reveal the spatiotemporal distribution of the cleavage products of the most abundant AMLEX splice variants including the full length P173, the shorter leucine-rich amelogenin protein (LRAP), and the exon 4-containing P190 in forming enamel, all within the context of the changing enamel matrix proteome during mineralization. We microsampled permanent pig molars, capturing known stages of enamel formation from both crown surface and inner enamel. Nano-LC-MS/MS proteomic analyses after tryptic digestion rendered more than 500 unique protein identifications in enamel, dentin, and bone. We mapped collagens, keratins, and proteolytic enzymes (CTSL, MMP2, MMP10) and determined distributions of P173, LRAP, and P190 products, the enamel proteins enamelin (ENAM) and ameloblastin (AMBN), and matrix-metalloprotease-20 (MMP20) and kallikrein-4 (KLK4). All enamel proteins and KLK4 were near-exclusive to enamel and in excellent agreement with published abundance levels. Phosphorylated P173 and LRAP products decreased in abundance from recently deposited matrix toward older enamel, mirrored by increasing abundances of testicular acid phosphatase (ACPT). Our results showed that hierarchical clustering analysis of secretory enamel links closely matching distributions of unphosphorylated P173 and LRAP products with ACPT and non-traditional amelogenesis proteins, many associated with enamel defects. We report higher protein diversity than previously published and Gene Ontology (GO)-defined protein functions related to the regulation of mineral formation in secretory enamel (e.g., casein α-S1, CSN1S1), immune response in erupted enamel (e.g., peptidoglycan recognition protein, PGRP), and phosphorylation. This study presents a novel approach to characterize and study functional relationships through spatiotemporal mapping of the ephemeral extracellular matrix proteome.

show abstract

Section: Resultsmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Mapping the Tooth Enamel Proteome and Amelogenin Phosphorylation Onto Mineralizing Porcine Tooth Crowns

Green¹,

Schulte²,

Lee

et al. 2019

Front. Physiol.

View full text Add to dashboard Cite

show abstract

“…We found that resistin, which is a hormone derived from macrophages in humans and adipose tissue in rodents, increases early in sepsis and remains increased during the acute phase of stress, related with the sepsis discriminators lactate and eHSP90α and also histidine and asparagine. In a follow‐up study, adiponectin increased, and resistin decreased during sepsis, but only the resistin related to the severity of sepsis and the degree of inflammatory response .…”

Section: Discussionmentioning

confidence: 90%

Longitudinal Profiles of Metabolism and Bioenergetics Associated with Innate Immune Hormonal Inflammatory Responses and Amino‐Acid Kinetics in Severe Sepsis and Systemic Inflammatory Response Syndrome in Children

Spanaki

Tavladaki

Dimitriou

et al. 2018

J Parenter Enteral Nutr

Self Cite

View full text Add to dashboard Cite

show abstract

“…The clinical conditions of critically ill patients are highly heterogeneous. It has been recently shown that not only malnutrition was associated with poor outcome [ 1 ] but also that energy expenditure was related with adenosine triphosphate concentration [ 2 ]. Therefore, nutritional intake should be tailored to the individual needs of the patient.…”

Section: Introductionmentioning

confidence: 99%

Targeted Energy Intake Is the Important Determinant of Clinical Outcomes in Medical Critically Ill Patients with High Nutrition Risk

Wang

Huang

et al. 2018

Nutrients

View full text Add to dashboard Cite

The clinical conditions of critically ill patients are highly heterogeneous; therefore, nutrient requirements should be personalized based on the patient’s nutritional status. However, nutritional status is not always considered when evaluating a patient’s nutritional therapy in the medical intensive care unit (ICU). We conducted a retrospective cross-sectional study to assess the effect of ICU patients’ nutrition risk status on the association between energy intake and clinical outcomes (i.e., hospital, 14-day and 28-day mortality). The nutrition risk of critically ill patients was classified as either high- or low-nutrition risk using the modified Nutrition Risk in the Critically Ill score. There were 559 (75.3%) patients in the high nutrition risk group, while 183 patients were in the low nutrition risk group. Higher mean energy intake was associated with lower hospital, 14-day and 28-day mortality rates in patients with high nutrition risk; while there were no significant associations between mean energy intake and clinical outcomes in patients with low nutrition risk. Further examination of the association between amount of energy intake and clinical outcomes showed that patients with high nutrition risk who consumed at least 800 kcal/day had significantly lower hospital, 14-day and 28-day mortality rates. Although patients with low nutrition risk did not benefit from high energy intake, patients with high nutrition risk are suggested to consume at least 800 kcal/day in order to reduce their mortality rate in the medical ICU.

show abstract

Similar Metabolic, Innate Immunity, and Adipokine Profiles in Adult and Pediatric Sepsis Versus Systemic Inflammatory Response Syndrome—A Pilot Study

Cited by 32 publications

References 40 publications

Mapping the Tooth Enamel Proteome and Amelogenin Phosphorylation Onto Mineralizing Porcine Tooth Crowns

Mapping the Tooth Enamel Proteome and Amelogenin Phosphorylation Onto Mineralizing Porcine Tooth Crowns

Longitudinal Profiles of Metabolism and Bioenergetics Associated with Innate Immune Hormonal Inflammatory Responses and Amino‐Acid Kinetics in Severe Sepsis and Systemic Inflammatory Response Syndrome in Children

Targeted Energy Intake Is the Important Determinant of Clinical Outcomes in Medical Critically Ill Patients with High Nutrition Risk

Contact Info

Product

Resources

About