Similar growth pattern of mouse mammary epithelium cultivated in collagen matrix in vivo and in vitro

Daniel, Charles W.; Berger, Joseph J.; Strickland, Phyllis; García, Richard

doi:10.1016/0012-1606(84)90036-8

Cited by 56 publications

(28 citation statements)

References 17 publications

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“…Early studies by Daniel et al (1984) using ex-vivo culture and fragment implants in collagen 1 gels showed that collagen was required for the regenerating mammary gland and perhaps for normal development. In these experiments, we show that in the absence of macrophages, much less fibrillar collagen surrounds the terminal end bud in the stroma.…”

Section: Discussionmentioning

confidence: 99%

Macrophages promote collagen fibrillogenesis around terminal end buds of the developing mammary gland

Ingman

Wyckoff

Gouon-Evans

et al. 2006

Developmental Dynamics

247

221

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Development of the ductal network in the mammary gland is dependent in part on the presence of macrophages. Here we utilize multi-photon microscopy and second harmonic generation to describe terminal end bud 3-dimensional structure and the organization of the surrounding collagen matrix. We have applied this approach to analyze the effect of macrophage deficiency on terminal end bud structure and collagen organization, using mice homozygous for a null mutation in the colony stimulating factor-1 gene (Csf1 op /Csf1 op ). Primary terminal end buds have an oblong shape, with long collagen I fibers close to the neck of the terminal end bud and radiating upwards in the direction of growth. Around the terminal end buds, the amount of total collagen I detected by antibody staining was not affected by macrophage deficiency. However the amount of collagen I organized into long fibers, detected by second harmonic generation signal, was reduced in Csf1 op /Csf1 op mice. Macrophage deficiency also caused terminal end buds to be rounder and shorter. These studies reveal a role for macrophages in collagen fibrillogenesis and in organization of the structure of terminal end buds. Developmental Dynamics 235:3222-3229, 2006.

show abstract

Section: Discussionmentioning

confidence: 99%

Macrophages promote collagen fibrillogenesis around terminal end buds of the developing mammary gland

Ingman

Wyckoff

Gouon-Evans

et al. 2006

Developmental Dynamics

247

221

View full text Add to dashboard Cite

show abstract

“…In the mouse, the development in the male and in the female follows the same pattern up to about the day 13 of embryonal life; at day 11, an area of raised ectoderm is formed on both sides of the trunk, and the neighbouring ectodermal cells begin to congregate around this area to form the mammary band (Fig. 1A).…”

Section: Embryogenesismentioning

confidence: 89%

“…In the female, at day 15 of embryonic life, the mammary bud cells undergo a phase of rapid proliferation, giving rise to the mammary cord, a Formation of the mammary cord in the mouse embryo between days 11 and 15 of gestation. (A) Mammary band, day 11; (B) mammary bud, day 12-13; (C) mammary cord, day 15. band of epithelial cells elongated into the mesenchyme but still connected to the epidermis by a collar of ectodermal cells (Fig.…”

Section: Embryogenesismentioning

confidence: 99%

“…During this phase the mammary epithelium in the female grows extensively and extends throughout the fat pad. Very few alveoli are formed at this time; the growth pattern is three-dimensional and can be simulated in cultures of mammary epithelium into a collagen matrix (11). Elongation of the ducts occurs as a result of rapid growth in end buds, which, by turning and branching, give rise to the characteristic tree-shaped pattern of the mammary ductal system.…”

Section: From Birth To Maturitymentioning

confidence: 99%

See 1 more Smart Citation

Growth Control and Differentiation in Mammary Epithelial Cells

Borellini¹,

Oka²

1989

Environmental Health Perspectives

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Growth and differentiation of the mammary gland are controlled by various hormones and other environmental factors The role of hormones and growth factors in mammary development is discussed with regard to animal species, physiological stages, and the various experimental systems in vitro. In the female embryo, mammary morphogenesis is induced by the mesenchyme and is hormone independent, whereas androgens cause the partial necrosis of mammary epithelium in the male. Ductal growth during adolescence requires estrogen and prolactin or growth hormone. During pregnancy, progesterone participates in the development of the lobuloaveolar structure of the gland. After parturition, changes in the hormonal environment lead to production and secretion of milk. Proliferation and differentiation of mammary epithelium can be induced in culture systems. Insulin and epidermal growth factor (EGF) stimulate mammary cell proliferation in vitro. EGF is required for the optimal growth of the mammary gland during pregnancy. EGF also appears to play an important role in mammary tumorigenesis in certain mouse strains. Production of milk proteins can be induced in vitro by the synergistic interactions of prolactin, insulin, and glucocorticoids and is inhibited by EGF and progesterone. Complete or partial sequencing of several milk protein genes and comparative analysis have led to identification of a sequence of high homology and conservation in the 5 'flanking region that is likely to be involved in the regulation of milk protein gene expression.

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“…It has been shown that various mammary epithelia are capable of morphogenic expression in cell-free collagen gels, or when embedded in hydrated collagen gels and implanted into the gland (13). This morphogenic expression, however, is not as extensive and is usually characterized by spiked ends rather than end buds typical of normal mammary morphogenesis in vivo (Fig.…”

Section: Mammary Gland Histogenesismentioning

confidence: 99%

Regulation of Functional Cytodifferentiation and Histogenesis in Mammary Epithelial Cells: Role of the Extracellular Matrix

Bissell¹,

Ram²

1989

Environmental Health Perspectives

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Primary mammary epithelial cells provide a versatile system for the study of hormone and extracellular matrix (ECM) influences on tissue-specific gene expression. We have characterized the formation of aveolarlike morphogenesis and mammary-specific functional differentiation that occur when these cells are cultured on a reconstituted basement membrane (EHS). Cells cultured on EHS exhibit many ultrastructural and biochemical features indicative of polarized and functionally differentiated mammary epithelium in vivo. The increased expression and specific vectorial secretion of milk proteins into lumina formed in culture are accompanied by large increases in milk protein mRNA expression. However, when individual ECM components are tested, smaller increases in milk protein mRNA are measured on heparan sulfate proteoglycan (HSPG) and laminin, and these responses are not associated with full functional cytodifferentiation or histotypic configuration. This indicates that multiple levels of regulation are involved in mammary-specific gene expression, and that in addition to individual ligand requirements cooperative interactions between various ECM molecules and cells are necessary for functional differentiation in culture. We have also shown that endogenous production of ECM molecules and changes in cell geometry are correlated with changes in functional and histogenic gene expression. We have previously proposed a model of cell-ECM interactions that is consistent with these data.

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Similar growth pattern of mouse mammary epithelium cultivated in collagen matrix in vivo and in vitro

Cited by 56 publications

References 17 publications

Macrophages promote collagen fibrillogenesis around terminal end buds of the developing mammary gland

Macrophages promote collagen fibrillogenesis around terminal end buds of the developing mammary gland

Growth Control and Differentiation in Mammary Epithelial Cells

Regulation of Functional Cytodifferentiation and Histogenesis in Mammary Epithelial Cells: Role of the Extracellular Matrix

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