Similar expression profiles of a core of genes and proteins in cells that have acquired a metastatic phenotype, genetically or by in vivo evolution

Abstract

We compared gene expression patterns related to cancer progression, cell adhesion and cytokine expression in v-Src-transformed isogenic cell lines with different levels of metastatic aggressiveness in syngenic animals: a high (HM) and a low metastatic (LM) cell lines, both predetermined by in vitro oncogenic transformation, and a high metastatic (NM) cell line resulting from in vivo selection of grafted LM cells. While LM and NM shared a common v-Src transforming variant different from that of HM, gene expression patterns were similar in HM and NM but different from that of LM. In this model, the induction of the metastatic phenotype by an oncogene variant or by the host environment led to highly similar expression profiles. If confirmed in other models, these results would be relevant for the diagnosis and treatment of metastasis.

Arf6 promotes cell proliferation via the PLD‐mTORC1 and p38MAPK pathways

“…The goal of this study was to study the Arf6 influence on cluster of properties involved in tumor progression and acquisition of highly aggressive cell phenotype, including mentioned above motility, invasiveness and metastatic potential. The chosen HET‐SR cell line is characterized by high level of tumorigenicity and low level of lung‐directed metastasis after subcutaneous injection in immunocompetent syngeneic animals [Deichman et al, 1989; Tatosyan et al, 1996; Illan Rubio et al, 2010]. Pointed features make this model especially attractive for studying influence of different proteins on SMA.…”

“…Here, we focused on Arf6 role in aggressive cellular phenotype acquisition, particularly, in modifying cell growth properties, motility, and invasion as well as on Arf6‐dependent intracellular signaling. HET‐SR, a well characterized cell line of RSV‐transformed Syrian hamster primary fibroblasts, was used as a convenient model for tumor progression and metastasis study since it is high tumorigenic and low metastatic in the spontaneous metastatic activity (SMA) test on immunocompetent syngeneic animals [Deichman et al, 1989; Tatosyan et al, 1996; Illan Rubio et al, 2010]. Previously, using this model system we have demonstrated SMA stimulating effect of few small GTPases and other proteins [Tchevkina et al, 2005].…”