Similar Differentiation Patterns Between PBP Expression Levels and Pheromone Component Ratios in Two Populations of Sesamia nonagrioides

Glaser, Nicolas; Frérot, Brigitte; Leppik, Ene; Monsempes, Christelle; Capdevielle‐Dulac, Claire; Rü, Bruno Le; Lecocq, Thomas; Harry, Myriam; Jacquin‐Joly, Emmanuelle; Calatayud, Paul‐André

doi:10.1007/s10886-014-0485-2

Cited by 8 publications

(9 citation statements)

References 15 publications

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“…The three homologous PBPs in the sibling species S. littoralis were also identified 40 , but the comparative analysis of their expression in male antennae was not reported, but we can suspect that the qualitative differences of the sex pheromone components and the three PBPs expressed in male antennae are important factors that permit correct mate recognition and sexual isolation in the two sibling species. This hypothesis was supported by a recently research, inverted expression ratio of PBP1 and PBP2 was found in two different geographical species of the stem borer Sesamia nonagrioides (Lepidoptera:Noctuidae), and this inverted expression ratio of PBP1 and PBP2 are correlated with the inverted ratio of their two sex pheromone components Z11-16:OH and Z11-16:Ald 49 . In addition, the three PBPs in S. littoralis were also expressed in the sensilla of the larvae antennae and suspected to be involved in foraging activity 50 .…”

Section: Discussionsupporting

confidence: 63%

Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura

Zhou

Gao

et al. 2015

Sci Rep

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Insect odorant binding proteins (OBPs) are thought to involve in insects' olfaction perception. In the present study, we identified 38 OBP genes from the antennal transcriptomes of Spodoptera litura. Tissue expression profiles analysis revealed that 17 of the 38 SlitOBP transcripts were uniquely or primarily expressed in the antennae of both sexes, suggesting their putative role in chemoreception. The RPKM value analysis revealed that seven OBPs (SlitPBP1-3, SlitGOBP1-2, SlitOBP3 and SlitOBP5) are highly abundant in male and female antennae. Most S. litura antennal unigenes had high homology with Lepidoptera insects, especially genes of the genus Spodoptera. Phylogenetic analysis of the Lepidoptera OBPs demonstrated that the OBP genes from the genus Spodoptera (S. litura, Spodoptera littoralis and Spodoptera exigua) had a relatively close evolutionary relationship. Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here. The motif-patterns between Lepidoptera OBPs and CSPs are also compared. The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.Insects use their sensitive and selective olfactory organs, usually their antennae, to smell the outside world. Species-specific pheromones and general plant volatiles can diffuse inside the sensilla via the multipores to penetrate the cuticular surface of antennae 1 . When the odorant molecules enter the sensillum lymph, two types of processes occur. First, perireceptor events 2 occur as the hydrophobic pheromones and/or the odorants bind to the soluble odorant binding proteins (OBPs), which deliver the pheromones and odorants to the membrane-bound olfactory receptors (ORs). After activating the ORs, the odorant molecules are subsequently quickly degraded by the odorant degrading enzymes (ODEs) 3 . After these processes, receptor events occur by the specific interaction of odorants with ORs, leading to the activation of the chemoelectric transduction machinery in the olfactory receptor neurons (ORNs) 4 . Although many researchers have focus on these two events, the events prior to membrane receptor stimulation are still poorly understood.Insect OBPs are highly concentrated in antennal sensilla and are proposed to be involved in the first biochemical step of perireceptor events in odorant reception 5,6 . Insect OBPs were first identified in the silkmoth Antheraea polyphemus, where they are uniquely expressed in male antennae and bind with the sex pheromones, so these OBPs are named as pheromone binding proteins (PBPs) 7 . Based on the

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Section: Discussionsupporting

confidence: 63%

Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura

Zhou

Gao

et al. 2015

Sci Rep

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“…The variation in the main compound differentiation within Alpinobombus taxa could be due to a switching off and on of specific metabolic pathways (Glaser et al 2014) and or by activation of an enzyme transcript present in a common ancestor (Roelofs et al, 2002). For instance, between CLGS of B. hyperboreus and B. neoboreus (two closely related species), the difference in the main compounds lays in the position of the C=C double bond (octadec-11-en-1-ol vs octadec-9-en-1-ol).…”

Section: Evolution Of Clgs In Alpinobombusmentioning

confidence: 99%

Adding attractive semio-chemical trait refines the taxonomy of Alpinobombus (Hymenoptera: Apidae)

et al. 2018

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Species taxonomy of bumblebees (Bombus Latreille, 1802) is well known to be problematic due to a potentially high intra-specific variability of morphological traits while different species can converge locally to the same color pattern (cryptic species). Assessing species delimitation remains challenging because it requires to arbitrarily select variable traits whose accuracy continues to be debated. Integrative taxonomic approach seems to be very useful for this group as different independent traits are assessed to propose a rational taxonomic hypothesis. Among operational criteria to assess specific status, the reproductive traits involved in the pre-mating recognition (i.e., the male cephalic labial gland secretions, CLGS) have been premium information. Since these secretions are supposed to be species-specific, these chemical traits can bring essential information where species delimitation is debated. Here, we describe and compare the CLGS of 161 male specimens of nine Alpinobombus taxa: alpinus , balteatus , helleri , hyperboreus , kirbiellus, natvigi , neoboreus , polaris , and pyrrhopygus. We aim also to test the congruence between this new information (reproductive traits) and published genetic dataset. Our results emphasized six distinct groups with diagnostic major compounds: (a) alpinus + helleri with hexadec-9-en-1-ol; (b) polaris + pyrrhopygus with two major compounds hexadec-9-en-1-ol and hexadec-9-enal; (c) balteatus with tetradecyl acetate; (d) kirbiellus with geranyl geranyl acetate; (e) hyperboreus + natvigi with octadec-11en-1-ol; (f) neoboreus with octadec-9-en-1-ol. Based on this new information, we can confirm the species status of B. alpinus , B. balteatus , B. hyperboreus , B. kirbiellus , B. neoboreus , and B. polaris . We also confirm the synonymy of helleri (Alps) with alpinus (Sweden). However, the specific status of natvigi (Alaska) and pyrrhopygus (Sweden) is questionable and these taxa do not have specific CLGS composition.bumblebees / species / cephalic labial gland secretions / arcto-alpine distribution / chemical trait

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“…We already reported the presence of PBAN transcripts in the head and thoracic complex of Maruca vitrata , but absent in abdomen (Chang & Ramasamy, ). The PBP transcripts were mostly expressed in antennae, as evidenced in S. nonagrioides (Glaser et al ., ). There was one isoform cluster identified as a putative PBAN gene, and two isoform clusters were homologous to PBAN‐R (Fig.…”

Section: Resultsmentioning

confidence: 97%

“…For instance, PBAN transcripts were present in the head and thoracic complex of legume pod borer ( Maruca vitrata ), a Crambid species (Chang & Ramasamy, ). The three PBP transcripts in the stem borer, Sesamia nonagrioides were found to be expressed in antennae (Glaser et al ., ). Hence, we focused on transcripts of S. recurvalis in the head (with antennae) and thoracic complex using Illumina sequencing technology.…”

Section: Introductionmentioning

confidence: 97%

Transcriptome analysis in the beet webworm, Spoladea recurvalis (Lepidoptera: Crambidae)

Chang¹,

Srinivasan

2016

Insect Science

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The beet webworm, Spoladea recurvalis Fabricius, is a destructive pest on vegetable crops in tropics and subtropics; its main host plant is amaranth. It has become imperative to develop non-chemical methods to control S. recurvalis on amaranth. However, the lack of molecular information about this species has hindered the development of novel pest management strategies. In this study, high-throughput RNA sequencing covering de novo sequence assemblies, functional annotation of transcripts, gene function classification and enrichment was performed on S. recurvalis. Illumina sequencing generated a total of 120 435 transcript contigs ranging from 201 to 22 729 bases with a mean length of 688 bases. The assembled transcripts were subjected to Basic Local Alignment Search Tool-X (BLASTX) to obtain the annotations against non-redundant, Swiss-Prot, Clusters of Orthologous Groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) protein databases. A subset of 58 225 transcript sequences returned hits from known proteins in the National Center for Biotechnology Information database, and the majority of the transcript sequences had the highest number of hits for Danaus plexippus (50.43%). A total of 1217 Gene Ontology-level 3 annotations were assigned to 51 805 transcripts, while 39 650 transcripts were predicted as functional protein-coding genes in the COG database and 20 037 transcripts were enriched to KEGG pathways. We identified 40 putative genes related to pheromone production and reception in S. recurvalis, with the expression of one gene between 0.29 and 1141.79 fragments per kilo base per million (FPKM) reads. The transcriptome sequence of S. recurvalis is a first step toward offering a comprehensive genomic resource which would enable better understanding of molecular mechanisms to enable development of effective pest management practices for this species.

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Similar Differentiation Patterns Between PBP Expression Levels and Pheromone Component Ratios in Two Populations of Sesamia nonagrioides

Cited by 8 publications

References 15 publications

Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura

Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura

Adding attractive semio-chemical trait refines the taxonomy of Alpinobombus (Hymenoptera: Apidae)

Transcriptome analysis in the beet webworm, Spoladea recurvalis (Lepidoptera: Crambidae)

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