Similar Diagnostic Performance for Neurocysticercosis of Three Glycoprotein Preparations From Taenia Solium Metacestodes

Abstract

The detection of antibodies to Taenia solium metacestodes is very important in the differential diagnosis of neurocysticercosis (NCC). In this study, an electroimmunotransfer blot (EITB) assay that uses an elaborate protocol with metacestode glycoproteins as antigens was compared with two other Western blots that use glycoproteins obtained using simpler methods, including an eluate from a lectin column, or the vesicular fluid (VF) of the parasite. The concordance between the three assays was 91% in patients with active NCC and 100% in patients with suspected NCC and previous documentation of negative serology. The specificities for the Western blots and the EITB assay were 98% and 100%, respectively (98% concordance). These data suggest that the simplest of these immunoassays, the one that uses the VF of T. solium metacestodes in a Western blot format, can be reliably used for the serologic diagnosis of NCC in developing countries where access to the EITB assay is difficult.

Increased Accumulation of Regulatory Granulocytic Myeloid Cells in Mannose Receptor C Type 1-Deficient Mice Correlates with Protection in a Mouse Model of Neurocysticercosis

“…In vivo studies using murine NCC further provide evidence that glycan antigens are released/secreted in a time-dependent fashion and that such N-glycans (WGA binding glycans) are taken up by infiltrating immune cells, some of which appear immunodominant by antibody production (9). Similarly, ex vivo studies have shown the presence of multiple immunodominant molecules present in N-glycan fractions of T. solium fluid, such as GP-12, -16, and -18 as well as others (11,26,45). However, there is little understanding about how these glycans are recognized and the subsequent immunological consequences.…”

Proteomic analysis of a 120 kDa protein complex in cyst fluid of Taenia solium metacestode and preliminary evaluation of its value for the serodiagnosis of neurocysticercosis

“…Representative results of the immunoblot assays are shown in Fig. It has also been reported that some of the LLGP, particularly those comprising the three major bands between 7 or 14 and 18 or 26 kDa, are regularly spaced and are frequently recognized simultaneously in the sera of NC patients (Restrepo et al 2000 ;Hancock et al 2003;Villota et al 2003). Positive antibody reactions against r14 and r18 were observed in 90 % and 85 % of the serum samples from the active and mixed stage NC (18/20 and 17/20 cases), whereas 2 and 1 of the 10 samples from chronic NC revealed positive reactions.…”

Taeniosis and Cysticercosis

“…Although glycoproteins-EITB has 100 % specificity and an overall sensitivity of 98 %, major problems are that approximately 30 % of patients with a single brain parasite, or calcified lesions, may test negative (Wilson et al 1991) and that the T. solium genotypes showed distinct glycoprotein patterns (Sato et al 2006). Also, it is important to note the technique is very expensive, hampering its use in endemic areas (Villota et al 2003;Suzuki and Rossi 2011).The EITB was recognized as the gold standard for NCC immunodiagnosis by Pan American Health Organization (PAHO) (Greene et al 2000). Also, purified T. solium E/S antigens have been employed in ELISA and FAST-ELISA with very promising results in antibody detection of NCC (Ng and Ko 1994;Sahu et al 2009;Atluri et al 2009).…”