Simil-Microfluidic Nanotechnology in Manufacturing of Liposomes as Hydrophobic Antioxidants Skin Release Systems

Abstract

Novel nanotechnologies represent the most attractive and innovative tools to date exploited by cosmetic companies to improve the effectiveness of their formulations. In this context, nanoliposomes have had a great impact in topical preparations and dermocosmetics, allowing the transcutaneous penetration and absorption of several active ingredients and improving the stability of sensitive molecules. Despite the recent boom of this class of delivery systems, their industrial production is still limited by the lack of easily scalable production techniques. In this work, nanoliposomes for the topical administration of vitamin D3, K2, E, and curcumin, molecules with high antioxidant and skin curative properties but unstable and poorly absorbable, were produced through a novel simil-microfluidic technique. The developed high-yield semi continuous method is proposed as an alternative to face the problems linked with low productive conventional methods in order to produce antioxidant formulations with improved features. The novel technique has allowed to obtain a massive production of stable antioxidant vesicles of an 84–145 nm size range, negatively charged, and characterized by high loads and encapsulation efficiencies. The obtained products as well as the developed high-performance technology make the achieved formulations very interesting for potential topical applications in the cosmetics/cosmeceutical field.

Herbal nanocosmecuticals: A review on cosmeceutical innovation

“…Liposome formulation inhibited melanin concentration in B16 melanoma cells and increased cell viability in HaCaT keratinocyte cells, according to a cellular investigation. In terms of skin penetration metrics and tyrosinase inhibition, Liposomal Phenylethyl Resorcinol cream demonstrated better stability and performance than Phenylethyl Resorcinol cream 38 …”

Formulation and Evaluation of Liposome Moringa Oleifera Seed Oil (Moringa oleifera L.) as Anti-aging

“…Particle size is one of the factors for increasing the permeability, thereby increasing the amount of penetration of the active substance in the preparation in the stratum corneum. This is due to the liposome component as a penetration factor that can increase the permeability of the stratum corneum, because liposome vesicles are fused with the lipid components of the stratum corneum in the skin, so that the vesicles are able to penetrate deep into the skin layer [22]. Based on the results of the particle size analysis of the 9 liposome formulas of Moringa seed oil, the formula that has the best particle size is seen in formula 6 with a value of 83.98nm.…”

A New Productive Approach and Formulative Optimization for Curcumin Nanoliposomal Delivery Systems

“…To address the limitations of existing techniques for producing nanoliposomes—such as the need for harsh operating conditions, toxic solvents, multiple post-processing steps, poorly controlled conditions, low output volumes, and high costs associated with microfluidic devices—a new technology called “simil-microfluidic” has been developed [ 16 , 32 , 38 ]. This novel technology allows for the production of homogeneous, antioxidant-loaded nanoliposomes in a single step at room temperature, directly at the nanoscale.…”

“…In this study, several production batches were prepared. The first batch was produced using a recipe outlined in a previous study [ 16 ]. To prepare the lipid ethanol solution, 940 mg of PC, 188 mg of cholesterol, and 140 mg of curcumin were dissolved in 20 mL of ethanol, resulting in a theoretical concentration in CUR of 6.99 mg/mL.…”

“…They are useful as biocompatible drug-delivery vehicles for hydrophilic, amphiphilic, and lipophilic molecules which are poorly absorbed and/or rapidly metabolized in their naked form (such curcumin). Due to their low intrinsic toxicity and immunogenicity and their resemblance to cell membranes in terms of structure and composition, a characteristic that favors the drug penetration through biological barriers, liposomes are attractive candidates in the controlled release of many kinds of active ingredients [ 15 , 16 , 17 ]. Moreover, liposomes’ size, which has a key role in carriers’ uptake after their administration, can be easily modified according to need, i.e., it has flexible features.…”