Silybin has therapeutic efficacy against non-small cell lung cancer through targeting of Skp2

Zhang, Shibing; Hong, Ming; Sun, Xiaoyang; Huang, Dan; He, Dan‐Hua; Chen, Yufei; Yuan, Yong; Liu, Yongqiang

doi:10.15212/amm-2022-0011

Cited by 15 publications

(5 citation statements)

References 21 publications

(31 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The incidence of NSCLC has remained high, which is coupled with the 5-year survival rate remaining low. Pathological staging is particularly necessary for designating the treatment of NSCLC ( 138 , 139 ). Therefore, in addition to the current traditional imaging and pathological examination techniques, it is necessary to identify novel characteristic diagnostic biomarkers of NSCLC.…”

Section: Summary and Discussionmentioning

confidence: 99%

Long non‑coding RNAs as potential therapeutic targets in non‑small cell lung cancer (Review)

Tang

Sun

et al. 2023

Int J Mol Med

View full text Add to dashboard Cite

Non-small cell lung cancer (NSCLC) is one of the most common malignancies with a high morbidity and mortality rate. Long non-coding RNAs (lncRNAs) have been reported to be closely associated with the occurrence and progression of NSCLC. In addition, lncRNAs have been documented to participate in the development of drug resistance and radiation sensitivity in patients with NSCLC. Due to their extensive functional characterization, high tissue specificity and sex specificity, lncRNAs have been proposed to be novel biomarkers and therapeutic targets for NSCLC. Therefore, in the current review, the functional classification of lncRNAs were presented, whilst the potential roles of lncRNAs in NSCLC were also summarized. Various physiological aspects, including proliferation, invasion and drug resistance, were all discussed. It is anticipated that the present review will provide a perspective on lncRNAs as potential diagnostic molecular biomarkers and therapeutic targets for NSCLC.

show abstract

Section: Summary and Discussionmentioning

confidence: 99%

Long non‑coding RNAs as potential therapeutic targets in non‑small cell lung cancer (Review)

Tang

Sun

et al. 2023

Int J Mol Med

View full text Add to dashboard Cite

show abstract

“…Caliper measurements of tumor diameters were performed twice a week to estimate the tumor volume, using the following formula: 0.5 × width 2 × length. Blood was collected from the mouse eyeballs, and the serum was used to measure alanine transaminase (ALT), aspartate aminotransferase (AST), creatinine (CRE), and urea nitrogen (BUN) levels (S. Zhang et al, 2022). The liver, spleen, and kidney were immobilized, and H&E staining was performed (Wang et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

Polyphyllin VII induces autophagy‐dependent ferroptosis in human gastric cancer through targeting T‐lymphokine‐activated killer cell‐originated protein kinase

Xiang,

Wan,

Ren

et al. 2023

Phytotherapy Research

View full text Add to dashboard Cite

T‐lymphokine‐activated killer cell‐originated protein kinase (TOPK) is a serine–threonine kinase that is overexpressed in gastric cancer (GC) and promotes tumor progression. Polyphyllin VII (PPVII), a pennogenin isolated from the rhizomes of Paris polyphylla, shows anticancer effects. Here, we explored the antitumor activity and mechanism of PPVII in GC. Ferroptosis was detected by transmission electron microscope, malondialdehyde, and iron determination assays. Autophagy and its upstream signaling pathway were detected by Western blot, and gene alterations. The binding of PPVII and TOPK was examined through microscale thermophoresis and drug affinity responsive target stability assays. An in vivo mouse model was performed to evaluate the therapeutic of PPVII. PPVII inhibits GC by inducing autophagy‐mediated ferroptosis. PPVII promotes the degradation of ferritin heavy chain 1, which is responsible for autophagy‐mediated ferroptosis. PPVII activates the Unc‐51‐like autophagy‐activating kinase 1 (ULK1) upstream of autophagy. PPVII inhibits the activity of TOPK, thereby weakening the inhibition of downstream ULK1. PPVII stabilizes the dimer of the inactive form of TOPK by direct binding. PPVII inhibits tumor growth without causing obvious toxicity in vivo. Collectively, this study suggests that PPVII is a potential agent for the treatment of GC by targeting TOPK to activate autophagy‐mediated ferroptosis.

show abstract

“…We lysed the cell pellet using a mixture of RIPA lysis buffer and protease inhibitor on ice. The protein quantification was performed using bicinchoninic acid assay, followed by electrophoresis and transfer as previously described [ 21 ]. The antibodies (human specific) for immunoblotting namely cyclin B1 (12231), CDK1(9116), p-CDC25C (Ser216, 4901), p-Histone H3 (Ser10, 3377S), PARP (9542), caspase 3 (9662S), caspase 9 (9502) and Bcl-2 (15071S) were supplied by Cell signaling Technology Company (Danvers, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Pulsatilla Decoction and its bioactive component β-peltatin induce G2/M cell cycle arrest and apoptosis in pancreatic cancer

Liu

et al. 2023

Chin Med

View full text Add to dashboard Cite

Background Pancreatic cancer (PAC), a malignancy that is fatal and commonly diagnosed at a late stage. Despite considerable advancements in cancer treatment, the survival rate of PAC remains largely consistent for the past 60 years. The traditional Chinese medicine formula Pulsatilla Decoction (PD) has been clinically used to treat inflammatory diseases for millennia and recently as a supplementary anti-cancer treatment in China. However, the bioactive ingredients and mechanisms underlying its anti-cancer effect remains unclear. Methods The composition and quality control of PD were verified through analysis by high performance liquid chromatography. Cell viability was determined using Cell Counting Kit-8 assay. The cell cycle distribution was analyzed through PI staining and flow cytometry analysis, while apoptotic cells were measured by double staining with Annexin V-FITC and PI. We used immunoblotting to examine protein expressions. The in vivo effects of β-peltatin and podophyllotoxin were evaluated on a subcutaneously-xenografted BxPC-3 cell nude mice model. Results The current study demonstrated that PD markedly inhibited PAC cell proliferation and triggered their apoptosis. Four herbal PD formula was then disassembled into 15 combinations of herbal ingredients and a cytotoxicity assay showed that the Pulsatillae chinensis exerted the predominant anti-PAC effect. Further investigation indicated that β-peltatin was potently cytotoxic with IC50 of ~ 2 nM. β-peltatin initially arrested PAC cells at G2/M phase, followed by apoptosis induction. Animal study confirmed that β-peltatin significantly suppressed the growth of subcutaneously-implanted BxPC-3 cell xenografts. Importantly, compared to podophyllotoxin that is the parental isomer of β-peltatin but clinically obsoleted due to its severe toxicity, β-peltatin exhibited stronger anti-PAC effect and lower toxicity in mice. Conclusions Our results demonstrate that Pulsatillae chinensis and particularly its bioactive ingredient β-peltatin suppress PAC by triggering cell cycle arrest at G2/M phase and apoptosis.

show abstract

Silybin has therapeutic efficacy against non-small cell lung cancer through targeting of Skp2

Cited by 15 publications

References 21 publications

Long non‑coding RNAs as potential therapeutic targets in non‑small cell lung cancer (Review)

Long non‑coding RNAs as potential therapeutic targets in non‑small cell lung cancer (Review)

Polyphyllin VII induces autophagy‐dependent ferroptosis in human gastric cancer through targeting T‐lymphokine‐activated killer cell‐originated protein kinase

Pulsatilla Decoction and its bioactive component β-peltatin induce G2/M cell cycle arrest and apoptosis in pancreatic cancer

Contact Info

Product

Resources

About