Silver potentiates aminoglycoside toxicity by enhancing their uptake

Hérisse, Marion; Duverger, Yohann; Martin‐Verstraete, Isabelle; Barras, Frédéric; Ezraty, Benjamin

doi:10.1111/mmi.13687

Cited by 30 publications

(54 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The potentiating activity of silver on antibiotic toxicity in E. coli K12 was further investigated by Herisse et al [ 4 ]. An extended set of bactericidal and bacteriostatic antibiotics including tetracycline and chloramphenicol were tested [ 4 ].…”

Section: Silver Enhances Antibacterial Activity Of Antibioticsmentioning

confidence: 99%

“…More recently, a series of initiatives aimed at fighting multidrug resistant bacteria elected combinatorial strategies as a way of potentiating drug efficiency [ 2 ]. Likewise, silver ions were identified as a highly efficient potent of antibiotics of different classes [ 3 , 4 ]. Last, silver nanoparticles (AgNP) rank currently among the most widely commercialised nanomaterial used in medical, bactericidal and electrical products [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Silver and Antibiotic, New Facts to an Old Story

2018

Self Cite

View full text Add to dashboard Cite

The therapeutic arsenal against bacterial infections is rapidly shrinking, as drug resistance spreads and pharmaceutical industry are struggling to produce new antibiotics. In this review we cover the efficacy of silver as an antibacterial agent. In particular we recall experimental evidences pointing to the multiple targets of silver, including DNA, proteins and small molecules, and we review the arguments for and against the hypothesis that silver acts by enhancing oxidative stress. We also review the recent use of silver as an adjuvant for antibiotics. Specifically, we discuss the state of our current understanding on the potentiating action of silver ions on aminoglycoside antibiotics.

show abstract

Section: Silver Enhances Antibacterial Activity Of Antibioticsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Silver and Antibiotic, New Facts to an Old Story

2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Silver is an inert metal in its metallic form; however, it is biologically active when it is in the ionic monoatomic state (Ag + ) soluble in an aqueous environment (water or tissue fluids) [6]. This activated ion shows a strong affinity for sulfhydryl groups and protein residues present in cell membranes [6].Previous studies established four main mechanisms of action of silver ions: (i) destabilization of the cell membrane through the binding of silver ions to the sulfur atoms present in sulfhydryl groups of proteins and enzymes located on the bacterial cell surface [6-8], (ii) production of reactive oxygen species (ROS) [7,9], (iii) inhibition of metabolic pathways through the binding of silver ions to any protein that has some sulfur atom function [10], and (iv) interaction with bacterial DNA which causes the breakdown of cell cycle [11].Recent studies reported that silver potentiates the antibacterial activity of ampicillin, ofloxacin, gentamicin, tetracycline, and chloramphenicol against E. coli in vitro and in animal models [7,12], tobramycin against biofilm producing E. coli and P. aeruginosa [13], and vancomycin against E. coli [7]. Moreover, silver was demonstrated to treat, as an adjuvant, persister cells which are tolerant to antibiotics [7].…”

mentioning

confidence: 99%

“…Recent studies reported that silver potentiates the antibacterial activity of ampicillin, ofloxacin, gentamicin, tetracycline, and chloramphenicol against E. coli in vitro and in animal models [7,12], tobramycin against biofilm producing E. coli and P. aeruginosa [13], and vancomycin against E. coli [7]. Moreover, silver was demonstrated to treat, as an adjuvant, persister cells which are tolerant to antibiotics [7].…”

mentioning

confidence: 99%

Antibacterial Activity of Colloidal Silver against Gram-Negative and Gram-Positive Bacteria

et al. 2020

View full text Add to dashboard Cite

Due to the emergence of antimicrobial resistance, new alternative therapies are needed. Silver was used to treat bacterial infections since antiquity due to its known antimicrobial properties. Here, we aimed to evaluate the in vitro activity of colloidal silver (CS) against multidrug-resistant (MDR) Gram-negative and Gram-positive bacteria. A total of 270 strains (Acinetobacter baumannii (n = 45), Pseudomonas aeruginosa (n = 25), Escherichia coli (n = 79), Klebsiella pneumoniae (n = 58)], Staphylococcus aureus (n = 34), Staphylococcus epidermidis (n = 14), and Enterococcus species (n = 15)) were used. The minimal inhibitory concentration (MIC) of CS was determined for all strains by using microdilution assay, and time–kill curve assays of representative reference and MDR strains of these bacteria were performed. Membrane permeation and bacterial reactive oxygen species (ROS) production were determined in presence of CS. CS MIC90 was 4–8 mg/L for all strains. CS was bactericidal, during 24 h, at 1× and 2× MIC against Gram-negative bacteria, and at 2× MIC against Gram-positive bacteria, and it did not affect their membrane permeabilization. Furthermore, we found that CS significantly increased the ROS production in Gram-negative with respect to Gram-positive bacteria at 24 h of incubation. Altogether, these results suggest that CS could be an effective treatment for infections caused by MDR Gram-negative and Gram-positive bacteria.

show abstract

“…The MIC were determined as previously described (51). Briefly, each antibiotic containing-well of a 96-well micro-titer plate was inoculated with 100 μL of a fresh LB bacterial inoculum of 2 × 10 5 c.f.u / mL.…”

Section: Methodsmentioning

confidence: 99%

A small RNA controls bacterial resistance to gentamicin during iron starvation

Chareyre

Barras

Mandin

2018

Preprint

Self Cite

View full text Add to dashboard Cite

19Phenotypic resistance describes a bacterial population that becomes 20 transiently resistant to an antibiotic without requiring a genetic change. We here 21 investigated the role of the small regulatory RNA (sRNA) RyhB, a key contributor to 22 iron homeostasis, in the phenotypic resistance of Escherichia coli to various classes 23 of antibiotics. We found that RyhB induces resistance to gentamicin, an 24 aminoglycoside that targets the ribosome, when iron is scarce. RyhB induced 25 resistance is due to the inhibition of respiratory complexes Nuo and Sdh activities. 26 These complexes, which contain numerous Fe-S clusters, are crucial for generating a 27 proton motive force (pmf) that allows gentamicin uptake. RyhB directly represses the 28 expression of nuo and sdh operons by binding to their mRNAs, thereby inhibiting their 29 translation. Indirectly, RyhB also inhibits the maturation of Nuo and Sdh by repressing 30 synthesis of the Isc Fe-S biogenesis machinery. Notably, our study identifies nuo as a 31 new direct RyhB target and shows that respiratory complexes activity levels are 32 predictive of the bacterial sensitivity to gentamicin. Altogether, these results unveil a 33 new role for RyhB in the adaptation to antibiotic stress, an unprecedented 34 consequences of its role in iron starvation stress response. 35 36 3 37 AUTHOR'S SUMMARY 38 Understanding the mechanisms at work behind bacterial antibiotic resistance has 39 become a major health issue in the face of the antibiotics crisis. Here, we show that 40 RyhB, a bacterial small regulatory RNA, induces resistance of Escherichia coli to the 41 antibiotic gentamicin when iron is scarce, an environmental situation prevalent during 42 host-pathogen interactions. This resistance is due to RyhB repression of the 43 synthesis and post-translational maturation of the respiratory complexes Nuo and 44Sdh. These complexes are crucial in producing the proton motive force that allows 45 uptake of the antibiotics in the cell. Altogether, these data point out to a major role for 46 RyhB in escaping antibacterial action. 47 71 5 enhances resistance to aminoglycosides, a well-known class of antibiotics that target 72 the ribosome (7). This resistance is due to a deficiency in the maturation of the 73 respiratory complexes in isc mutants, which in turn leads to a decrease in the proton 74 motive force (pmf) that is essential for aminoglycosides uptake (15). Incidentally, it 75 was deduced from these results that the Suf machinery is unable to maturate 76 efficiently the Fe-S cluster containing proteins of the respiratory complexes, although 77 the molecular reason for this still remains unclear. Overall this study predicted that an 78 environmental signal that induces the switch from Isc to Suf should induce a transient 79 resistance to aminoglycosides. 80 Iron starvation is one signal that decreases the expression of the isc operon 81 encoding the Isc pathway. The small RNA RyhB mediates this regulation.(16). RyhB 82 is one of the most studied sRNAs to date in E...

show abstract

Silver potentiates aminoglycoside toxicity by enhancing their uptake

Abstract: and aminoglycoside uptake assays, we showed that silver potentiates aminoglycoside 36 action in by-passing the PMF-dependent step, but depended upon protein translation. 37We showed that oxidative stress or Fe-S cluster destabilization were not mandatory

Cited by 30 publications

References 30 publications

Silver and Antibiotic, New Facts to an Old Story

Silver and Antibiotic, New Facts to an Old Story

Antibacterial Activity of Colloidal Silver against Gram-Negative and Gram-Positive Bacteria

A small RNA controls bacterial resistance to gentamicin during iron starvation

Contact Info

Product

Resources

About