In this study, for the first time an electrochemiluminescence sensor for ultra-trace monitoring of lamotrigine is reported. The sensing probe was fabricated using silver prussian blue analogue as a new effective coreaction accelerator and carboxylated sulfur-doped graphitic carbon nitride nanocomposite as a new green luminophore. Also potassium peroxydisulfate was utilized as a strong co-reactant. The proposed ECL sensor exhibited excellent wide linear range (3.45 × 10 À 15 to 2.98 × 10 À 8 M), low detection limit (4.89 × 10 À 16 M), the relative standard deviation of 1.07 %, outstanding reproducibility and superior long-term stability. The prominent advantages of the sensor showed that it can be used to determine LMT in clinical samples.