Silver nanoparticles: Significance of physicochemical properties and assay interference on the interpretation of in vitro cytotoxicity studies

Ahmed, Kausar Begam Riaz; Nagy, Amber; Brown, Ronald P.; Zhang, Qin; Malghan, S.G.; Goering, Peter L.

doi:10.1016/j.tiv.2016.10.012

Cited by 193 publications

(108 citation statements)

References 170 publications

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“…There are several review papers published to address the issues associated with AgNPs in terms of their toxicology properties during their use as antimicrobial agents for textiles, dental biomaterials, and bio‐detectors, as well as during their syntheses . For instance, toxicity properties including cytotoxicity and genotoxicity of capped or uncapped AgNPs have been reviewed in detail .…”

Section: Introductionmentioning

confidence: 99%

A Review of Silver Nanoparticles: Research Trends, Global Consumption, Synthesis, Properties, and Future Challenges

Syafiuddin

Salmiati

Salim

et al. 2017

J Chinese Chemical Soc

328

176

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Silver nanoparticles (AgNPs) are intensively investigated for their superior physical, chemical, and biological properties. A proper knowledge of these properties is essential to maximizing the potential applications of AgNPs in several areas while minimizing their risks to humans and the environment. This paper aims to critically review AgNPs from the perspectives of research trends, global consumption, synthesis, properties, and future challenges. Generally, AgNPs can be synthesized using three methods, namely physical, chemical, and biological, and the related works as well as their numerous advantages and disadvantages are presented in this review. In addition, AgNPs can be potentially explored for various applications. Future challenges on (AgNP) synthesis, their release into the environment, and scaling up production, as presented in the review, suggest that several potential topics for future works are available to promote a safer and more efficient use of these nanoparticles. Studies on AgNPs in Malaysia have increased since the Malaysian government officially established a directorate for nanotechnology development. This calls for a proper set of policies on AgNPs starting from their production to utilization as well as their effects on various related industries and the environment.

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Section: Introductionmentioning

confidence: 99%

A Review of Silver Nanoparticles: Research Trends, Global Consumption, Synthesis, Properties, and Future Challenges

Syafiuddin

Salmiati

Salim

et al. 2017

J Chinese Chemical Soc

328

176

View full text Add to dashboard Cite

show abstract

“…Titanium dioxide has also been shown to be toxic to eukaryotic cells as studies have shown that cells exposed to high concentrations of TiO 2 nanoparticles had irregular cellular morphology, degraded DNA, increased evidence of oxidative stress, and damaged intracellular organelles (Jin, Zhu, Wang, & Lu, ; Liu, Xu, & Li, ). It seems these cytotoxic effects increase with decreasing nanoparticle diameter, which has also been demonstrated with silver nanoparticles (Liu et al., ; Riaz Ahmed et al., ). This may be due to the smaller sizes facilitating easier access across cellular membranes into the cytoplasm (Riaz Ahmed et al., ).…”

Section: Nanotechnology and Materials Science In Food Processingmentioning

confidence: 62%

“…It seems these cytotoxic effects increase with decreasing nanoparticle diameter, which has also been demonstrated with silver nanoparticles (Liu et al., ; Riaz Ahmed et al., ). This may be due to the smaller sizes facilitating easier access across cellular membranes into the cytoplasm (Riaz Ahmed et al., ). The value of antimicrobial elemental nano‐deposits on surfaces of food processing equipment may be dependent on their stability on the surface.…”

Section: Nanotechnology and Materials Science In Food Processingmentioning

confidence: 62%

“…The ability of silver to bind weak bases, such as sulfur and phosphate, can interfere with many intracellular processes in eukaryotic cells (Prabhu & Poulose, ). This includes disrupting the thiol groups in super oxide dismutase proteins leading to increased oxidative stress in the cell, targeting cytoplasmic and mitochondrial membrane proteins which leads to the disruption of the transmembrane potential and membrane integrity, and binding to DNA causing a structural transition to 1 of 3 bio‐inactive states (Arakawa, Neault, & Tajmir‐Riahi, ; Riaz Ahmed et al., ). Titanium dioxide has also been shown to be toxic to eukaryotic cells as studies have shown that cells exposed to high concentrations of TiO 2 nanoparticles had irregular cellular morphology, degraded DNA, increased evidence of oxidative stress, and damaged intracellular organelles (Jin, Zhu, Wang, & Lu, ; Liu, Xu, & Li, ).…”

Section: Nanotechnology and Materials Science In Food Processingmentioning

confidence: 99%

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Integration of Emerging Biomedical Technologies in Meat Processing to Improve Meat Safety and Quality

Ravensdale

Coorey

Dykes

2018

Comp Rev Food Sci Food Safe

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Modern-day processing of meat products involves a series of complex procedures designed to ensure the quality and safety of the meat for consumers. As the size of abattoirs increases, the logistical problems associated with large-capacity animal processing can affect the sanitation of the facility and the meat products, potentially increasing transmission of infectious diseases. Additionally, spoilage of food from improper processing and storage increases the global economic and ecological burden of meat production. Advances in biomedical and materials science have allowed for the development of innovative new antibacterial technologies that have broad applications in the medical industry. Additionally, new approaches in tissue engineering and nondestructive cooling of biological specimens could significantly improve organ transplantation and tissue grafting. These same strategies may be even more effective in the preservation and protection of meat as animal carcasses are easier to manipulate and do not have the same stringent requirements of care as living patients. This review presents potential applications of emerging biomedical technologies in the food industry to improve meat safety and quality. Future research directions investigating these new technologies and their usefulness in the meat processing chain along with regulatory, logistical, and consumer perception issues will also be discussed.

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“…AgNPs toxicity may be attributed to either nonspecific interactions, such as size and shape, or specific interactions with cells, such as surface coating, chemical composition and the release of Ag + ions (Riaz Ahmed et al, 2017). The size of the AgNPs is a main factor in mediating various biological effects such as oxidative stress, DNA damage, cellular uptake, mitochondrial dysfunction, and permeabilization across biological barriers (Gliga et al, 2014;Wang et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Effects on cytotoxicity and antibacterial properties of the incorporations of silver nanoparticles into the surface coating of dental alloys

Shen

Zhang

Xiao

et al. 2017

J. Zhejiang Univ. Sci. B

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Abstract:The aim of this study was to research the changes in cytotoxicity and antibacterial properties after silver nanoparticles (AgNPs) were incorporated into the surface coating of dental alloys. AgNPs were attached to cobalt chromium alloys and pure titanium using a hydrothermal method, according to the reaction: AgNO 3 +NaBH 4 → Ag+1/2H 2 +1/2B 2 H 6 +NaNO 3 . A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate the cytotoxicity of the alloys when in contact with osteogenic precursor cells (MC3T3-E1) from mice and mesenchymal stem cells (BMSC) from rats. The antibacterial properties of dental alloys incorporating three different concentrations (10, 4, and 2 μmol/L) of AgNPs were tested on Staphylococcus aureus (SA) and Streptococcus mutans (MS). High cytotoxicity values were observed for all dental alloys that contained 0% of AgNPs (the control groups). The incorporation of AgNPs reduced cytotoxicity values. No significant difference was observed for antibacterial performance when comparing dental alloys containing AgNPs to the respective control groups. The results demonstrated that the cobalt chromium alloys and pure titanium all had cytotoxicity to MC3T3-E1 and BMSC and that the incorporation of AgNPs could reduce this cytotoxicity. The concentrations of AgNPs adopted in this study were found to have no antibacterial action against SA or MS.

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Silver nanoparticles: Significance of physicochemical properties and assay interference on the interpretation of in vitro cytotoxicity studies

Cited by 193 publications

References 170 publications

A Review of Silver Nanoparticles: Research Trends, Global Consumption, Synthesis, Properties, and Future Challenges

A Review of Silver Nanoparticles: Research Trends, Global Consumption, Synthesis, Properties, and Future Challenges

Integration of Emerging Biomedical Technologies in Meat Processing to Improve Meat Safety and Quality

Effects on cytotoxicity and antibacterial properties of the incorporations of silver nanoparticles into the surface coating of dental alloys

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