Silver nanoparticles inkjet-printed flexible biosensor for rapid label-free antibiotic detection in milk

Rosati, Giulio; Ravarotto, Marco; Scaramuzza, Matteo; Toni, A. De; Paccagnella, A.

doi:10.1016/j.snb.2018.09.084

Cited by 78 publications

(30 citation statements)

References 33 publications

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“…We assessed both the conductivity and the ratio between the real and nominal printed lines in previous research [21] that was focused on the fabrication of interdigitated electrodes (IDE) for antibiotic detection. Here, we also studied the lines' real width as a function of the line angle, since the sensor layout was more complicated and heterogeneous.…”

Section: Methodsmentioning

confidence: 99%

“…The inkjet-printed lines produced with the Epson printer were characterized in previous studies by means of their actual width and their conductivity [21]. However, this was done only for lines printed in the print head movement direction (horizontal lines).…”

Section: Printed Line Characterizationmentioning

confidence: 99%

“…Many substrates have been proposed and tested in the literature to be used with this technique for biosensing applications. Among them there are sustainable materials like paper, with tunable and peculiar microfluidic characteristics [11][12][13][14][15], and polyethylene terephthalate (PET), which is flexible, light, and transparent [16][17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

“…We used consumer low-cost printers to fabricate the devices. In a previous work, we studied and characterized the resistivity, resolution, and morphology of these devices [21]. In this research we focused on the electrical equivalent modeling of the impedance response of the printed devices, optimizing their geometrical parameters, and defining their stability for long-term measurements.…”

Section: Introductionmentioning

confidence: 99%

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Inkjet Printed Interdigitated Biosensor for Easy and Rapid Detection of Bacteriophage Contamination: a Preliminary Study for Milk Processing Control Applications

Rosati

Cunego²,

Fracchetti³

et al. 2019

Chemosensors

Self Cite

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Bacteriophages are responsible for significant material and time losses in the dairy industry. This because these viruses infect the selected lactic starter cultures used for milk fermentation, i.e., the first stage toward cheese production. Standard detection techniques are time- and labor-consuming, causing huge costs related to production plant sanitation and product wasting. A new type of biosensor for early detection of bacteriophage contamination is highly demanded by the milk processing market, and inkjet-printed electrochemical sensors could be the answer. Inkjet printing is a well-known technology that has been revisited in recent years, using silver nanoparticle (AgNP) based inks for low-cost and easy fabrication of sensing and biosensing systems on flexible and eco-compatible substrates. In this research, we studied inkjet printing for the manufacturing of both interdigitated electrodes arrays (IDEAs), and a versatile system to monitor bacterial cultures by electrochemical impedance spectroscopy (EIS). In particular, we studied this biosensing system for the detection of bacteriophages by comparing its performance with standard microbiological methods. We performed electrical and morphological characterizations of the devices produced with a consumer-use inkjet printer with commercial AgNPs ink on flexible substrates, such as office paper, polyethylene (PET), and photo paper. We used light microscopy optical analysis, profilometry, atomic force microscopy (AFM), and scanning electron microscopy (SEM) imaging to define the objects resolution, their real dimensions, and thickness. We also investigated the devices’ conductivity and layout, by EIS measurements with a standard buffer solution, i.e., phosphate buffered saline (PBS). Finally, we tested our system by monitoring Lactococcus lactis cultures and bacteriophage infection. We compared the results to those obtained by two standard microbiological methods in terms of response time, proving that our technique requires less than half the time of other methods and no specialized personnel.

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Section: Methodsmentioning

confidence: 99%

Section: Printed Line Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inkjet Printed Interdigitated Biosensor for Easy and Rapid Detection of Bacteriophage Contamination: a Preliminary Study for Milk Processing Control Applications

Rosati

Cunego²,

Fracchetti³

et al. 2019

Chemosensors

Self Cite

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“…The newly developed inkjet technologies have demonstrated their precise control capability of droplets (Castrejon-Pita et al, 2013), for example, the acoustic drop generation (Elrod et al, 1989) (Hadimioglu et al n.d.) and cavity collapse (Silverbrook, 2011) methods. Rosati et al (2019) designed an inkjet-printed flexible biosensor for rapid label-free antibiotic detection. Kastner et al, (2019) introduced a new method for production of printable graphene flakes applied in organic solar cells.…”

Section: Introductionmentioning

confidence: 99%

Theoretical Design of Inkjet Process to Improve Delivery Efficiency

Zhong¹,

Dong²,

Yin³

et al. 2020

JAFM

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Inkjet technology is an essential tool for precise and quick delivery of liquids in micro-droplets. A key topic of the technology is to deliver the droplets efficiently by designing the nozzle that is related to the droplet speed and the droplet volume in a stable inkjet process. The ejected droplets are usually too small to determine their physical states through onsite measurement. Complex physical phenomena, such as the coupling effects of surface tension, viscous force and inertial force, make it difficult to optimize the nozzle design by experiments alone. In the paper, we adopt computational fluid dynamics to investigate the inkjet process with the orthogonal test method to arrange the studied cases. The computational results firstly have been verified through measuring a simulated case that could be observed in the experiment. Different nozzle structures then have been examined by numerical simulation. It is found that the Laval-shaped nozzle can improve the droplet speed significantly to deliver the droplets fast, and that the curvilinear-triangle-shaped nozzle can minimize the droplet volume to improve the printing accuracy. It is further revealed that a large ink viscosity and surface tension, as well as a low ink density can improve the process stability. Additionally, a parameter combined by the droplet speed, the droplet volume and the stability level is proposed to evaluate the comprehensive performance of the inkjet nozzle.

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Bioreceptors for Cells

Prajapati,

Roy

2023

Biosensors Nanotechnology

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Silver nanoparticles inkjet-printed flexible biosensor for rapid label-free antibiotic detection in milk

Cited by 78 publications

References 33 publications

Inkjet Printed Interdigitated Biosensor for Easy and Rapid Detection of Bacteriophage Contamination: a Preliminary Study for Milk Processing Control Applications

Inkjet Printed Interdigitated Biosensor for Easy and Rapid Detection of Bacteriophage Contamination: a Preliminary Study for Milk Processing Control Applications

Theoretical Design of Inkjet Process to Improve Delivery Efficiency

Bioreceptors for Cells

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