Silver nanoparticles induce anti-proliferative effects on airway smooth muscle cells. Role of nitric oxide and muscarinic receptor signaling pathway

Ramirez-Lee, Manuel Alejandro; Rosas-Hernández, Héctor; Salazar-García, Samuel; Gutiérrez-Hernández, José Manuel; Espinosa‐Tanguma, Ricardo; González, Francisco; Ali, Syed F.; González, Carmen

doi:10.1016/j.toxlet.2013.10.027

Cited by 24 publications

(19 citation statements)

References 68 publications

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“…However, unmodified NMs such as carbon black (14 nm) also exhibited an intrinsic capacity to bind to EGFR receptors to activate the Akt signalling cascade, stimulating proliferation of the RLE6TN cells . In contrast, Ag NPs exerted anti‐proliferative effects on rat airway smooth muscle cells, caused by their unintended interaction with the cells' muscarinic receptors . Gadolinium endohedral metallofullerenols (Gd@C 82 (OH) 22 ), purported to be potential antitumor chemotherapeutics due to their low toxicity to noncancer cells, could still interact with Toll‐like receptors (TLRs) and this interaction promoted pro‐inflammation cytokine IL‐1β production .…”

Section: Some New Yin and Yang Relationships Of Nms With Biological Pmentioning

confidence: 68%

Back to Basics: Exploiting the Innate Physico‐chemical Characteristics of Nanomaterials for Biomedical Applications

Tay

Setyawati

Xie

et al. 2014

Adv Funct Materials

200

182

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In nanomedicine design, emphasis is centered on the engineered impacts of the nanomaterials (NMs). However, failure to understand the unintended effects of nanomaterials on the cell biology can affect the overall performance, approval, and adoption in the clinic. Much of these unintended effects arise from unique physico-chemical properties of the NMs. This feature article discusses some of the key physico-chemical parameters of NMs and highlights how they could cause unexpected and novel biological responses, with some insights into their underlying mechanisms.

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Section: Some New Yin and Yang Relationships Of Nms With Biological Pmentioning

confidence: 68%

Back to Basics: Exploiting the Innate Physico‐chemical Characteristics of Nanomaterials for Biomedical Applications

Tay

Setyawati

Xie

et al. 2014

Adv Funct Materials

200

182

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“…Their dissolution rates are affected by surface-bound moieties and the local environments (Choi et al, 2009;Wang et al, 2013). Cellular uptake can have multiple results including cell death, DNA damage (Kruszewski et al, 2013;Sahu et al, 2014a), increased oxidative stress (Kruszewski et al, 2013) and altered function (Ramírez- Lee et al, 2014). Given these potential adverse interactions, we are interested in improving our understanding of nAg in medical device coatings and improving our ability to make accurate risk assessments of these technologies.…”

Section: Introductionmentioning

confidence: 99%

Different cytotoxicity responses to antimicrobial nanosilver coatings when comparing extract‐based and direct‐contact assays

Sussman

Casey

Dutta

et al. 2015

J of Applied Toxicology

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This study was performed to understand how the choice of cytotoxicity assay format affects the observed biocompatibility of nanosilver (nAg). nAg coatings are physical coatings containing silver (Ag) that have feature sizes of 100 nm or less, often in the form of nanoparticles or grains. They are used on medical devices to prevent infection, but in spite of this intended benefit, observations of potential cytotoxicity from nAg have been reported in numerous published studies. For medical device regulation, cytotoxicity testing is part of a biocompatibility evaluation, in which specific test methods are chosen based on the technological characteristics and intended use of a device. For this study, nAg-coated tissue culture polystyrene surfaces were prepared using magnetron sputter coating, resulting in nAg films of 0.2 to 311 µg cm(-2) Ag. These coatings exhibited nanometer-scale morphologies and demonstrated a > 4log10 reduction in Escherichia coli viability. It was observed that extracts of nAg caused no cytotoxicity to L929 mouse fibroblasts, but cells cultured directly on nAg coatings (direct-contact assay format) showed a dose-dependent reduction in viability by up to 100% (P < 0.001). Results using inductively coupled plasma mass spectrometry to measure Ag release suggested that extracts of nAg are not toxic because the dissolved Ag in those samples becomes less cytotoxic over time, probably owing to the reaction with cell culture media and serum (six-fold cytotoxicity reductions observed over a 24-h period). These findings highlight the potential value of direct-contact cytotoxicity testing for nAg in predicting biological interactions with cells or tissue in vivo.

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“…117 Any chemical compound that affects the viability and function of Leydig and Sertoli cells may affect spermatogenesis. Braydich-Stolle et al 71 have revealed that 10 µg/mL of AgNPs inhibited SSC proliferation in vitro. Zhang et al 108 have shown that lipopolysaccharide inhibits the expression of GDNF in Sertoli cells and prevents SSC self-renewal via downregulation of GDNF-targeted genes.…”

mentioning

confidence: 99%

“…Induction of apoptosis by AgNPs was mediated by oxidative stress in fibroblasts, and muscle and colon cells. [70][71][72] Recently, Kim et al 62 have shown that in MC3T3-E1 and PC12 cells, ROS generation is induced by AgNPs in a size-and concentrationdependent manner.…”

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confidence: 99%

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Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells

Zhang¹,

Choi²,

Han³

et al. 2015

IJN

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Background Silver nanoparticles (AgNPs) possess unique physical, chemical, and biological properties. AgNPs have been increasingly used as anticancer, antiangiogenic, and antibacterial agents for the treatment of bacterial infections in open wounds as well as in ointments, bandages, and wound dressings. The present study aimed to investigate the effects of two different sizes of AgNPs (10 nm and 20 nm) in male somatic Leydig (TM3) and Sertoli (TM4) cells and spermatogonial stem cells (SSCs). Methods Here, we demonstrate a green and simple method for the synthesis of AgNPs using Bacillus cereus culture supernatants. The synthesized AgNPs were characterized using ultraviolet and visible absorption spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and transmission electron microscopy (TEM). The toxicity of the synthesized AgNPs was evaluated by the effects on cell viability, metabolic activity, oxidative stress, apoptosis, and expression of genes encoding steroidogenic and tight junction proteins. Results AgNPs inhibited the viability and proliferation of TM3 and TM4 cells in a dose- and size-dependent manner by damaging cell membranes and inducing the generation of reactive oxygen species, which in turn affected SSC growth on TM3 and TM4 as feeder cells. Small AgNPs (10 nm) were more cytotoxic than medium-sized nanoparticles (20 nm). TEM revealed the presence of AgNPs in the cell cytoplasm and nucleus, and detected mitochondrial damage and enhanced formation of autosomes and autolysosomes in the AgNP-treated cells. Flow cytometry analysis using Annexin V/propidium iodide staining showed massive cell death by apoptosis or necrosis. Real-time polymerase chain reaction and western blot analyses indicated that in TM3 and TM4 cells, AgNPs activated the p53, p38, and pErk1/2 signaling pathways and significantly downregulated the expression of genes related to testosterone synthesis (TM3) and tight junctions (TM4). Furthermore, the exposure of TM3 and TM4 cells to AgNPs inhibited proliferation and self-renewal of SSCs. Conclusion Our results suggest that AgNPs exhibit size-dependent nanoreprotoxicity in male somatic cells and SSCs, strongly suggesting that applications of AgNPs in commercial products must be carefully evaluated. Further studies of AgNPs-induced nanoreprotoxicity in animal models are required.

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Silver nanoparticles induce anti-proliferative effects on airway smooth muscle cells. Role of nitric oxide and muscarinic receptor signaling pathway

Cited by 24 publications

References 68 publications

Back to Basics: Exploiting the Innate Physico‐chemical Characteristics of Nanomaterials for Biomedical Applications

Back to Basics: Exploiting the Innate Physico‐chemical Characteristics of Nanomaterials for Biomedical Applications

Different cytotoxicity responses to antimicrobial nanosilver coatings when comparing extract‐based and direct‐contact assays

Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells

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