Silver nanoparticles for biolistic transformation in Nicotiana tabacum L.

Nitasana, Rajkumari.; Alex, Swapna; Soni, Kartik; Anith, K. N.; Viji, M.; Kiran, A. G.

doi:10.1007/s13205-021-03043-9

Cited by 6 publications

(5 citation statements)

References 25 publications

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“…Examples of assisted delivery techniques in plant nanoparticle applications are shown in Figure 2 and described briefly in Table 3 . The biolistic (gene gun) is a device that uses an external force, such as compressed gas or helium, to propel nanoparticles [ 93 , 94 ]. Rajkumari et al reported the use of Ag NPs as gene carriers, replacing Au microcarriers for biolistic gene delivery in Nicotiana tabacum L., and showed that the transformation efficiency was significantly higher with Ag NPs than Au microparticles as carriers [ 94 ].…”

Section: Transport Of Nanoparticles Into Plantsmentioning

confidence: 99%

“…The biolistic (gene gun) is a device that uses an external force, such as compressed gas or helium, to propel nanoparticles [ 93 , 94 ]. Rajkumari et al reported the use of Ag NPs as gene carriers, replacing Au microcarriers for biolistic gene delivery in Nicotiana tabacum L., and showed that the transformation efficiency was significantly higher with Ag NPs than Au microparticles as carriers [ 94 ]. Sonoporation involves the application of ultrasound waves to create temporary pores in plant cell membranes, allowing nanoparticles to enter the cells [ 95 ].…”

Section: Transport Of Nanoparticles Into Plantsmentioning

confidence: 99%

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Transport of Nanoparticles into Plants and Their Detection Methods

Sembada,

Lenggoro

2024

Nanomaterials

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Nanoparticle transport into plants is an evolving field of research with diverse applications in agriculture and biotechnology. This article provides an overview of the challenges and prospects associated with the transport of nanoparticles in plants, focusing on delivery methods and the detection of nanoparticles within plant tissues. Passive and assisted delivery methods, including the use of roots and leaves as introduction sites, are discussed, along with their respective advantages and limitations. The barriers encountered in nanoparticle delivery to plants are highlighted, emphasizing the need for innovative approaches (e.g., the stem as a new recognition site) to optimize transport efficiency. In recent years, research efforts have intensified, leading to an evendeeper understanding of the intricate mechanisms governing the interaction of nanomaterials with plant tissues and cells. Investigations into the uptake pathways and translocation mechanisms within plants have revealed nuanced responses to different types of nanoparticles. Additionally, this article delves into the importance of detection methods for studying nanoparticle localization and quantification within plant tissues. Various techniques are presented as valuable tools for comprehensively understanding nanoparticle–plant interactions. The reliance on multiple detection methods for data validation is emphasized to enhance the reliability of the research findings. The future outlooks of this field are explored, including the potential use of alternative introduction sites, such as stems, and the continued development of nanoparticle formulations that improve adhesion and penetration. By addressing these challenges and fostering multidisciplinary research, the field of nanoparticle transport in plants is poised to make significant contributions to sustainable agriculture and environmental management.

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Section: Transport Of Nanoparticles Into Plantsmentioning

confidence: 99%

Section: Transport Of Nanoparticles Into Plantsmentioning

confidence: 99%

Transport of Nanoparticles into Plants and Their Detection Methods

Sembada,

Lenggoro

2024

Nanomaterials

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“…Carbon nanotube (CNT) NPs efficiently deliver DNA into arugula, wheat, and cotton, which results in high protein expression levels [109]. AgNPs (100 nm) act as a gene carrier efficiently in Nicotiana tabacum L. compared to gold microcarriers (0.6 micron) via the biolistic transformation method under conditions of varying helium pressure (1100 psi, 900 psi, 650 psi, and 450 psi) and target distance (9 cm and 6 cm) and produced the highest number of transient GUS expression [110]. Engineered cobalt ferrite NPs (Tb, Co, and Fe) and MnFe 2 O 4 magnetic NPs (MNPs) were hydroponically applied to barley, and it was found that NPs translocated from the roots to the leaves and increased plant growth by incorporating iron within tissues and modulating photoluminescence.…”

Section: Nanotechnology In Genetic Modificationmentioning

confidence: 99%

Nanotechnology in Food and Plant Science: Challenges and Future Prospects

Ansari

2023

Plants

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Globally, food safety and security are receiving a lot of attention to ensure a steady supply of nutrient-rich and safe food. Nanotechnology is used in a wide range of technical processes, including the development of new materials and the enhancement of food safety and security. Nanomaterials are used to improve the protective effects of food and help detect microbial contamination, hazardous chemicals, and pesticides. Nanosensors are used to detect pathogens and allergens in food. Food processing is enhanced further by nanocapsulation, which allows for the delivery of bioactive compounds, increases food bioavailability, and extends food shelf life. Various forms of nanomaterials have been developed to improve food safety and enhance agricultural productivity, including nanometals, nanorods, nanofilms, nanotubes, nanofibers, nanolayers, and nanosheets. Such materials are used for developing nanofertilizers, nanopesticides, and nanomaterials to induce plant growth, genome modification, and transgene expression in plants. Nanomaterials have antimicrobial properties, promote plants’ innate immunity, and act as delivery agents for active ingredients. Nanocomposites offer good acid-resistance capabilities, effective recyclability, significant thermostability, and enhanced storage stability. Nanomaterials have been extensively used for the targeted delivery and release of genes and proteins into plant cells. In this review article, we discuss the role of nanotechnology in food safety and security. Furthermore, we include a partial literature survey on the use of nanotechnology in food packaging, food safety, food preservation using smart nanocarriers, the detection of food-borne pathogens and allergens using nanosensors, and crop growth and yield improvement; however, extensive research on nanotechnology is warranted.

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“…Суть метода заключается в том, что молекулы ДНК наносят на поверхность носителей -золотых или вольфрамовых микрочастиц (83-95) (табл. 1) либо наночастиц (96), которые разгоняются до скорости 300-600 м/с за счет величины давления гелия. Ими осуществляют бомбардировку различных эксплантов, размещенных на мишени.…”

Section: классификация прямых методов генетической трансформации раст...unclassified

TRANSGENIC TOMATO PLANTS (Solanum lycopersicum L.): DIRECT METHODS OF GENE TRANSFER AND FACTORS AFFECTING TRANSFORMATION EFFICIENCY (review)

Mikhel¹

2022

S-h. biol.

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Томат (Solanum lycopersicum L.) -важнейшая продовольственная культура, которая также находит широкое применение в качестве модельного объекта в различных молекулярногенетических исследованиях, затрагивающих вопросы вегетативного развития и репродуктивной биологии, механизмов устойчивости растений к абиотическим и биотическим стрессам, ассоциативного симбиоза с микроорганизмами и многие другие, имеющие как фундаментальное, так и прикладное значение. Получение трансгенных растений томата, экспрессирующих чужеродные гетерологичные гены, а также растений с индуцированным сайленсингом или нокаутом собственных генов, -важная составляющая исследований в современной физиологии растений. Существует два принципиально отличающихся друг от друга подхода для введения чужеродной ДНК в геном томата. Первый (метод агробактериальной, или Agrobacterim-опосредованной, трансформации) основан на естественном механизме заражения растений бактериальным патогеном рода Agrobacterim (A. tumefaciens или A. rhizogenes) и опосредованном им переносе чужеродной ДНК в растительный геном. Второй подход основан на непосредственной доставке чужеродной ДНК в растительную клетку сквозь плазмалемму с помощью химических веществ (Ca 2+ , полиэтиленгликоль -ПЭГ) или физических воздействий (электрический импульс или повышенное давление) (так называемые прямые методы генетической трансформации томата). При этом трансгенные растения томата можно получать как классическим способом с использованием метода культуры изолированных органов и тканей in vitro, так и без него (трансформация in planta). В представленном обзоре рассмотрены классические методы прямого введения чужеродной ДНК в геном томата (химически опосредованная трансфекция, электропорация протопластов, микроинъекция, биобаллистическая трансформация), а также методы трансформации томата in planta (метод пыльцевых трубок или pollen-tube pathway, электропорация зародышей зрелых семян) и проведен подробный анализ физических, генетических и физиологических факторов, влияющих на эффективность трансформации (ЭТ). Обсуждаются особенности получения растений томата как с транзиентной экспрессией трансгена, так и со стабильно наследуемой вставкой в ядерный или пластидный геном. Отдельно рассмотрено применение прямых методов генетической трансформации для доставки различных систем геномного редактирования (ZFNs, TALEN, CRISPR/Cas, редакторов оснований, prime editing), получивших широкое распространение в последние 5 лет. Ключевые слова: Solanum lycopersicum L., электропорация, ПЭГ-опосредованная трансформация, микроинъекция, биобаллистическая трансформация, трансформация in planta, геномное редактирование.  Работа выполнена при финансовой поддержке Министерства науки и высшего образования Российской Федерации в рамках государственного задания № 0431-2022-0003.

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Silver nanoparticles for biolistic transformation in Nicotiana tabacum L.

Cited by 6 publications

References 25 publications

Transport of Nanoparticles into Plants and Their Detection Methods

Transport of Nanoparticles into Plants and Their Detection Methods

Nanotechnology in Food and Plant Science: Challenges and Future Prospects

TRANSGENIC TOMATO PLANTS (Solanum lycopersicum L.): DIRECT METHODS OF GENE TRANSFER AND FACTORS AFFECTING TRANSFORMATION EFFICIENCY (review)

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