Silver nanoparticles enhance survival of white spot syndrome virus infected Penaeus vannamei shrimps by activation of its immunological system

Meza, Alba Rocío Ochoa; Sánchez, Ana Ruth Álvarez; Romo-Quiñonez, Carlos R.; Barraza, Aarón; Magallón‐Barajas, Francisco J.; Chávez-Sánchez, Alexis; García‐Ramos, Juan Carlos; Toledano-Magaña, Yanis; Bogdanchikova, Nina; Pestryakov, Alexey; Mejía-Ruíz, Claudio Humberto

doi:10.1016/j.fsi.2018.10.007

Cited by 52 publications

(28 citation statements)

References 37 publications

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“…Our research group has studied a commercially available PVP-AgNP formulation known as Argovit™ that has been very effective as an antimicrobial [14, 15], antifungal [15], and antiviral agent [16–18]. This formulation also has been a determining factor in the rapid healing of diabetic foot ulcers [19].…”

Section: Introductionmentioning

confidence: 99%

Antiproliferative and Antitumour Effect of Nongenotoxic Silver Nanoparticles on Melanoma Models

Valenzuela-Salas

Girón-Vázquez

García‐Ramos

et al. 2019

Oxidative Medicine and Cellular Longevity

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During the last 3 decades, there has been a slow advance to obtain new treatments for malignant melanoma that improve patient survival. In this work, we present a systematic study focused on the antiproliferative and antitumour effect of AgNPs. These nanoparticles are fully characterized, are coated with polyvinylpyrrolidone (PVP), and have an average size of 35±15 nm and a metallic silver content of 1.2% wt. Main changes on cell viability, induction of apoptosis and necrosis, and ROS generation were found on B16-F10 cells after six hours of exposure to AgNPs (IC50=4.2 μg/mL) or Cisplatin (IC50=2.0 μg/mL). Despite the similar response for both AgNPs and Cisplatin on antiproliferative potency (cellular viability of 53.95±1.88 and 53.62±1.04) and ROS production (20.27±1.09% and 19.50±0.35%), significantly different cell death pathways were triggered. While AgNPs induce only apoptosis (45.98±1.88%), Cisplatin induces apoptosis and necrosis at the same rate (22.31±1.72% and 24.07±1.10%, respectively). In addition to their antiproliferative activity, in vivo experiments showed that treatments of 3, 6, and 12 mg/kg of AgNPs elicit a survival rate almost 4 times higher (P<0.05) compared with the survival rate obtained with Cisplatin (2 mg/kg). Furthermore, the survivor mice treated with AgNPs do not show genotoxic damage determined by micronuclei frequency quantification on peripheral blood cells. These results exhibit the remarkable antitumour activity of a nongenotoxic AgNP formulation and constitute the first advance toward the application of these AgNPs for melanoma treatment, which could considerably reduce adverse effects provoked by currently applied chemotherapeutics.

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Section: Introductionmentioning

confidence: 99%

Antiproliferative and Antitumour Effect of Nongenotoxic Silver Nanoparticles on Melanoma Models

Valenzuela-Salas

Girón-Vázquez

García‐Ramos

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…Taken together, these results suggest that Argovits-4 does not induce the expression in of the assayed early stage immune-related genes, suggesting that the antiviral activity or immunostimulation by AgNP involves a different route to the endocytic pathway. A previous study by our group (Ochoa-Meza et al, 2019) reported that the LGBP and Cu,ZnSOD genes are negatively deregulated by the presence of AgNPs (Argovit R ), which suggested that there must be a path for the internalization of the nanoparticles and the activation of the immune system of haemocytes. However, such a path does not require the participation of any of the five genes analysed in this study.…”

Section: Discussionmentioning

confidence: 93%

“…Recently, Juárez-Moreno et al (2017) reported that prophylactic doses 50 ng/g Argovit R (AgNPs-based formulation) administered by intramuscular route in infected shrimp with WSSV evinced a rate survival of 80% without toxic effects after 96 h post infection, while Ochoa-Meza et al (2019) showed evidence that a single therapeutic dose 1.2 µg/g AgNPs can delay the onset of clinical signs and enhance the response of shrimp immune system without toxic effects in healthy shrimps. However, this results are not comparable with Juárez-Moreno et al (2017) and Ochoa-Meza et al (2019) in effectiveness, due to the different: (1) AgNP preparations used, (2) weights of the shrimp and, (3) routes of exposure; considering that intramuscular administration is impractical in aquaculture due to the enormous quantity of organisms that are need to be treated (Kumar & Roy, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…This result indicated that a single dose of silver nanoparticles was able to increase shrimp survival by up to 80% without toxic effects. In another study, Ochoa-Meza et al (2019) evaluated the ability of silver nanoparticles coated with commercial non-toxic PVP (Argovit R ) to promote the immune system response of shrimp infected with WSSV and observed a late immunostimulatory activity of two immune-related genes. In the same report, authors showed that a single dose of AgNP was able to increase the survival of the shrimp against severe infections of WSSV, even under adverse conditions in the presence of a high concentration of ferrous ions.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of a new Argovit as an antiviral agent included in feed to protect the shrimpLitopenaeus vannameiagainst White Spot Syndrome Virus infection

Romo-Quiñonez

Sánchez

Álvarez-Ruíz

et al. 2020

PeerJ

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In this study, four experimental assays were conducted to evaluate the use of a new silver nanoparticle formulation named Argovit-4, which was prepared with slight modifications to enhance its biological activity against white spot syndrome virus (WSSV) in shrimp culture. The goals of these assays were to (1) determine the protective effect of Argovit-4 against WSSV, (2) determine whether Argovit-4 supplemented in feed exhibits toxicity towards shrimp, (3) determine whether Argovit-4 as antiviral additive in feed can prevent or delay/reduce WSSV-induced shrimp mortality, and (4) determine whether Argovit-4 supplemented in feed alters the early stages of the shrimp immune response. In bioassay 1, several viral inocula calibrated at 7 SID50(shrimp infectious doses 50% endpoint) were exposed to 40, 100, 200 and 1,000 ng/SID50 of Ag+ and then intramuscularly injected into shrimp for 96 h. In bioassay 2, shrimp were fed Argovit-4 supplemented in feed at different concentrations (10, 100 and 1,000 µg per gram of feed) for 192 h. In bioassay 3, shrimp were treated with Argovit-4 supplemented in feed at different concentrations and then challenged against WSSV for 192 h. In bioassay 4, quantitative real-time RT-qPCR was performed to measure the transcriptional responses of five immune-relevant genes in haemocytes of experimental shrimp treated with Argovit-4 supplemented in feed at 0, 6, 12, 24 and 48 h. The intramuscularly injected Argovit-4 showed a dose-dependent effect (p < 0.05) on the cumulative shrimp mortality from 0–96 h post-infection. In the second bioassay, shrimp fed Argovit-4 supplemented in feed did not show signs of toxicity for the assayed doses over the 192-h experiment. The third and fourth bioassays showed that shrimp challenged with WSSV at 1,000 µg/g feed exhibited reduced mortality without altering the expression of some immune system-related genes according to the observed level of transcriptional. This study is the first show that the new Argovit-4 formulation has potential as an antiviral additive in feed against WSSV and demonstrates a practical therapeutic strategy to control WSSV and possibly other invertebrate pathogens in shrimp aquaculture.

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“…Silver nanoparticles (AgNPs) have shown high antimicrobial activity; several studies have demonstrated the potential of AgNPs to eliminate pathogens like bacteria, fungi, viruses and parasites affecting humans [ 1 , 2 , 3 , 4 , 5 , 6 ]. For this reason, the effectiveness of AgNPs as antimicrobials in controlling pathogens in aquaculture has recently been investigated, showing that AgNPs can eliminate bacteria [ 7 , 8 ] viruses [ 9 , 10 ] and parasites [ 11 , 12 ] in fish and aquatic invertebrates. Thus, AgNPs may be used as a potential therapeutic agent to control pathogens in aquaculture [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons from Caenorhabditis elegans

Pimentel-Acosta

Ramírez-Salcedo

Morales-Serna

et al. 2020

IJMS

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The mechanisms of action of silver nanoparticles (AgNPs) in monogenean parasites of the genus Cichlidogyrus were investigated through a microarray hybridization approach using genomic information from the nematode Caenorhabditis elegans. The effects of two concentrations of AgNPs were explored, low (6 µg/L Ag) and high (36 µg/L Ag). Microarray analysis revealed that both concentrations of AgNPs activated similar biological processes, although by different mechanisms. Expression profiles included genes involved in detoxification, neurotoxicity, modulation of cell signaling, reproduction, embryonic development, and tegument organization as the main biological processes dysregulated by AgNPs. Two important processes (DNA damage and cell death) were mostly activated in parasites exposed to the lower concentration of AgNPs. To our knowledge, this is the first study providing information on the sub-cellular and molecular effects of exposure to AgNPs in metazoan parasites of fish.

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Silver nanoparticles enhance survival of white spot syndrome virus infected Penaeus vannamei shrimps by activation of its immunological system

Cited by 52 publications

References 37 publications

Antiproliferative and Antitumour Effect of Nongenotoxic Silver Nanoparticles on Melanoma Models

Antiproliferative and Antitumour Effect of Nongenotoxic Silver Nanoparticles on Melanoma Models

Evaluation of a new Argovit as an antiviral agent included in feed to protect the shrimpLitopenaeus vannameiagainst White Spot Syndrome Virus infection

Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons from Caenorhabditis elegans

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