Silver nanoparticles boost charge-extraction efficiency in
            <i>Shewanella</i>
            microbial fuel cells

Cao, Bocheng; Zhao, Zipeng; Peng, Lele; Shiu, Hui-Ying; Ding, Mengning; Song, Frank; Guan, Xun; Lee, Calvin K.; Huang, Jin; Zhu, Donghui; Fu, Xiaoyang; Wong, Gerard C. L.; Liu, Chong; Nealson, Kenneth H.; Weiss, Paul S.; Duan, Xiangfeng; Huang, Yu

doi:10.1126/science.abf3427

Cited by 221 publications

(109 citation statements)

References 44 publications

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“…A single cell electron collector permits intimate association of the conductive nanoparticles with the cellular electron transfer machinery and maximizes the interfacial area between microbial cells and electrodes, which substantially enhanced high electron transfer rate and BES performance. Similarly, by introducing trans-outermembrane silver nanoparticles, Cao et al (2021) obtained the highest power density of Shewanella MFCs reported to date. Collectively, all of these results demonstrate a promising and new platform for wiring-up cells with abiotic interface.…”

Section: Artificial Biofilmmentioning

confidence: 95%

Biofilm Biology and Engineering of Geobacter and Shewanella spp. for Energy Applications

Wang

Han

et al. 2021

Front. Bioeng. Biotechnol.

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Geobacter and Shewanella spp. were discovered in late 1980s as dissimilatory metal-reducing microorganisms that can transfer electrons from cytoplasmic respiratory oxidation reactions to external metal-containing minerals. In addition to mineral-based electron acceptors, Geobacter and Shewanella spp. also can transfer electrons to electrodes. The microorganisms that have abilities to transfer electrons to electrodes are known as exoelectrogens. Because of their remarkable abilities of electron transfer, Geobacter and Shewanella spp. have been the two most well studied groups of exoelectrogens. They are widely used in bioelectrochemical systems (BESs) for various biotechnological applications, such as bioelectricity generation via microbial fuel cells. These applications mostly associate with Geobacter and Shewanella biofilms grown on the surfaces of electrodes. Geobacter and Shewanella biofilms are electrically conductive, which is conferred by matrix-associated electroactive components such as c-type cytochromes and electrically conductive nanowires. The thickness and electroactivity of Geobacter and Shewanella biofilms have a significant impact on electron transfer efficiency in BESs. In this review, we first briefly discuss the roles of planktonic and biofilm-forming Geobacter and Shewanella cells in BESs, and then review biofilm biology with the focus on biofilm development, biofilm matrix, heterogeneity in biofilm and signaling regulatory systems mediating formation of Geobacter and Shewanella biofilms. Finally, we discuss strategies of Geobacter and Shewanella biofilm engineering for improving electron transfer efficiency to obtain enhanced BES performance.

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Section: Artificial Biofilmmentioning

confidence: 95%

Biofilm Biology and Engineering of Geobacter and Shewanella spp. for Energy Applications

Wang

Han

et al. 2021

Front. Bioeng. Biotechnol.

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“…Representative examples of 2D and 3D electrode interface layers designed to increase current densities are listed in Table 1. [56][57][58][59][60][61][62][63][64][65][66][67] Although 2D electrode interface layers in MESs usually generate lower absolute biotic current density compared with their 3D counterparts, we found that the improvement of current density using our PEDOT:PSS/PHEA-coated electrode surpassed that of other 2D and 3D interfaces. For example, a 2018 study reported that the steady-state current density increased a factor of 20 using a PEDOT:PSS-based, multilayer biocomposite interface layer.…”

Section: Poly(34-ethylenedioxythiophene)-poly(styrenesulfonate)/ Poly...mentioning

confidence: 99%

Solution‐Deposited and Patternable Conductive Polymer Thin‐Film Electrodes for Microbial Bioelectronics

et al. 2022

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Microbial bioelectronic devices integrate naturally occurring or synthetically engineered electroactive microbes with microelectronics. These devices have a broad range of potential applications, but engineering the biotic–abiotic interface for biocompatibility, adhesion, electron transfer, and maximum surface area remains a challenge. Prior approaches to interface modification lack simple processability, the ability to pattern the materials, and/or a significant enhancement in currents. Here, a novel conductive polymer coating that significantly enhances current densities relative to unmodified electrodes in microbial bioelectronics is reported. The coating is based on a blend of poly(3,4‐ethylenedioxythiophene)‐poly(styrenesulfonate) (PEDOT:PSS) crosslinked with poly(2‐hydroxyethylacrylate) (PHEA) along with a thin polydopamine (PDA) layer for adhesion to an underlying indium tin oxide (ITO) electrode. When used as an interface layer with the current‐producing bacterium Shewanella oneidensis MR‐1, this material produces a 178‐fold increase in the current density compared to unmodified electrodes, a current gain that is higher than previously reported thin‐film 2D coatings and 3D conductive polymer coatings. The chemistry, morphology, and electronic properties of the coatings are characterized and the implementation of these coated electrodes for use in microbial fuel cells, multiplexed bioelectronic devices, and organic electrochemical transistor based microbial sensors are demonstrated. It is envisioned that this simple coating will advance the development of microbial bioelectronic devices.

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“…[173,174] Electrogenic bacteria are known for their extraordinary capability to electrochemically interact with external redox-active materials, which could promote seamless contact between biological systems and external electronics. Conventionally, such living electrodes are obtained by natural biofilm formation, [171,175,176] increasing the surface-area-to-volume ratio of the electrode, [20,[177][178][179][180] or modifying the electrode surface free energy to favor bacterial attachment. [174,181] More recently, the conductive polymer poly(3,4-ethylenedioxythiophene) (PEDOT) gained considerable attention in the field of bioelectronics because of its biocompatibility, good electric and ionic conductivity, and chemical stability.…”

Section: Living Electrodesmentioning

confidence: 99%

“…[16][17][18][19] Many studies have explored ways to improve the MFC performance through nanotechnologies, genetic engineering of bacteria, and material innovations. [13,20,21] However, their integration into the large-scale footprint cost-effectively and robustly is questionable. Although stacking of modular Considerable research efforts into the promises of electrogenic bacteria and the commercial opportunities they present are attempting to identify potential feasible applications.…”

mentioning

confidence: 99%

Electrogenic Bacteria Promise New Opportunities for Powering, Sensing, and Synthesizing

Choi

2022

Small

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Considerable research efforts into the promises of electrogenic bacteria and the commercial opportunities they present are attempting to identify potential feasible applications. Metabolic electrons from the bacteria enable electricity generation sufficient to power portable or small‐scale applications, while the quantifiable electric signal in a miniaturized device platform can be sensitive enough to monitor and respond to changes in environmental conditions. Nanomaterials produced by the electrogenic bacteria can offer an innovative bottom‐up biosynthetic approach to synergize bacterial electron transfer and create an effective coupling at the cell–electrode interface. Furthermore, electrogenic bacteria can revolutionize the field of bioelectronics by effectively interfacing electronics with microbes through extracellular electron transfer. Here, these new directions for the electrogenic bacteria and their recent integration with micro‐ and nanosystems are comprehensively discussed with specific attention toward distinct applications in the field of powering, sensing, and synthesizing. Furthermore, challenges of individual applications and strategies toward potential solutions are provided to offer valuable guidelines for practical implementation. Finally, the perspective and view on how the use of electrogenic bacteria can hold immeasurable promise for the development of future electronics and their applications are presented.

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Silver nanoparticles boost charge-extraction efficiency in Shewanella microbial fuel cells

Cited by 221 publications

References 44 publications

Biofilm Biology and Engineering of Geobacter and Shewanella spp. for Energy Applications

Biofilm Biology and Engineering of Geobacter and Shewanella spp. for Energy Applications

Solution‐Deposited and Patternable Conductive Polymer Thin‐Film Electrodes for Microbial Bioelectronics

Electrogenic Bacteria Promise New Opportunities for Powering, Sensing, and Synthesizing

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