Silver nanoparticle loaded collagen/chitosan scaffolds promote wound healing via regulating fibroblast migration and macrophage activation

You, Chuangang; Li, Qiong; Wang, Xingang; Wu, Pan; Ho, Jon Kee; Jin, Ronghua; Zhang, Liping; Shao, Huawei; Han, Chunmao

doi:10.1038/s41598-017-10481-0

Cited by 227 publications

(144 citation statements)

References 34 publications

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“…Moreover, it inhibited inflammation in skin wounds (studies on C57BL/mice). You et al [250] fabricated collagen/chitosan scaffold modified with silver nanoparticles (NAg). The in vitro experiments showed that NAg/collagen/chitosan biomaterial possessed antibacterial activity against E. coli and S. aureus as well as promoted migration of skin cells (studies on co-culture of mouse embryo fibroblasts and human skin keratinocytes (HaCaT)).…”

Section: Hacat and Bj Cell Lines/ Micementioning

confidence: 99%

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

2020

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Polymer scaffolds constitute a very interesting strategy for tissue engineering. Even though they are generally non-toxic, in some cases, they may not provide suitable support for cell adhesion, proliferation, and differentiation, which decelerates tissue regeneration. To improve biological properties, scaffolds are frequently enriched with bioactive molecules, inter alia extracellular matrix proteins, adhesive peptides, growth factors, hormones, and cytokines. Although there are many papers describing synthesis and properties of polymer scaffolds enriched with proteins or peptides, few reviews comprehensively summarize these bioactive molecules. Thus, this review presents the current knowledge about the most important proteins and peptides used for modification of polymer scaffolds for tissue engineering. This paper also describes the influence of addition of proteins and peptides on physicochemical, mechanical, and biological properties of polymer scaffolds. Moreover, this article sums up the major applications of some biodegradable natural and synthetic polymer scaffolds modified with proteins and peptides, which have been developed within the past five years.

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Section: Hacat and Bj Cell Lines/ Micementioning

confidence: 99%

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

2020

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“…This technique is suitable to study the paracrine signals (conditioned media) produced by stem (10) or other types of cells under either mechanical (7) or electrical stimuli (11). Furthermore, it is possible to study the response of free cells and those seeded on scaffolds made of both synthetic polymers and natural extracellular matrices (7,(12)(13)(14). In this context, the in vitro scratch wound healing assay is useful to evaluate the proliferation and migration process when exposing cells to metabolites present in the conditioned media.…”

Section: Introductionmentioning

confidence: 99%

An image J plugin for the high throughput image analysis of in vitro scratch wound healing assays

Suarez‐Arnedo

Figueroa

Clavijo

et al. 2020

Preprint

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In vitro scratch wound healing assay, a simple and low-cost technique that works along with other image analysis tools, is one of the most widely used 2D methods to determine the cellular migration and proliferation in processes such as regeneration and disease. There are open-source programs such as imageJ to analyze images of in vitro scratch wound healing assays, but these tools require manual tuning of various parameters, which is time-consuming and limits image throughput. For that reason, we developed an optimized plugin for imageJ to automatically recognize the wound healing size, correct the average wound width by considering its inclination, and quantify other important parameters such as: area, wound area fraction, average wound width, and width deviation of the wound images obtained from a scratch/ wound healing assay. Our plugin is easy to install and can be used with different operating systems. It can be adapted to analyze both individual images and stacks.Additionally, it allows the analysis of images obtained from bright field, phase contrast, and fluorescence microscopes. In conclusion, this new imageJ plugin is a robust tool to automatically standardize and facilitate quantification of different in vitro wound parameters with high accuracy compared with other tools and manual identification.

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“…Because of the well‐known pronounced antimicrobial activity of silver nanoparticles (AgNPs), they may be used as an efficient nanofiller in wound dressings to eliminate various types of pathogens . In addition, AgNPs are able to regulate fibroblast migration and macrophage activation, thus promoting wound healing .…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of biomedical collagen–chitosan scaffolds with entrapped ibuprofen and silver nanoparticles

Rubina

Said-Galiev

Naumkin

et al. 2019

Polymer Engineering & Sci

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In this work, the preparation and characterization of a novel multifunctional collagen–chitosan material containing silver nanoparticles and nonsteroid anti‐inflammatory drug ibuprofen is described. As a starting material, the commercially available collagen–chitosan scaffold was used. Drug‐loading procedure was performed via supercritical CO2‐assisted impregnation technique. Silver nanoparticles were prepared via metal vapor synthesis and introduced into the composite biopolymer matrix. An analysis of the small‐angle X‐ray scattering profiles revealed that silver nanoparticles are characterized by having a logarithmic normal size distribution with the maximum at 25 nm. The average size of particles determined from the broadening of diffraction peaks is in a good agreement with this result. The surface of the materials was characterized by X‐ray photoelectron spectroscopy. The in vitro release kinetics of ibuprofen in phosphate buffer solution with pH = 7.4 from prepared materials was studied. The drug release to solution is governed by Fickian diffusion and can be described within the Ritger–Peppas model. Introduction of silver nanoparticles has no effect on the diffusion mechanism. The as‐prepared materials are promising for the medical applications such as dressings for treatments of infected wounds and infection preventing. These materials can act as a matrix for transdermal drug administration. POLYM. ENG. SCI., 59:2479–2487, 2019. © 2019 Society of Plastics Engineers

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Silver nanoparticle loaded collagen/chitosan scaffolds promote wound healing via regulating fibroblast migration and macrophage activation

Cited by 227 publications

References 34 publications

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

An image J plugin for the high throughput image analysis of in vitro scratch wound healing assays

Preparation and characterization of biomedical collagen–chitosan scaffolds with entrapped ibuprofen and silver nanoparticles

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