Silver nanoparticle-induced degranulation observed with quantitative phase microscopy

Yang, Wenzhong; Lee, Seungrag; Lee, Jiyong; Bae, Yoonsung; Kim, Dugyoung

doi:10.1117/12.842925

Cited by 3 publications

(3 citation statements)

References 16 publications

(32 reference statements)

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“…To the best of our knowledge, the present study's data demonstrating that AgNP 20 , but not AgNP 70 , promote degranulation in human PMN are the first to be reported in the literature. These results are in agreement with previous studies demonstrating AgNP could induce degranulation in rodent mast cells Yang et al 2010). Although not of human origin, these cells were found to degranulate in response to AgNP with a diameter of 20 nm (at 25 and 50 mg/ml), but not of 110 nm .…”

Section: Discussionsupporting

confidence: 93%

Silver nanoparticles of 70 nm and 20 nm affect differently the biology of human neutrophils

Poirier

Simard

Girard

2015

Journal of Immunotoxicology

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The influence of size of nanoparticles (NP), especially in regard to pulmonary toxicity, has been widely investigated. In general, NP with smaller diameters are more pro-inflammatory in vivo, at least in terms of neutrophil influx. Nevertheless, the influence of size of NP on polymorphonuclear neutrophil (PMN) cell biology is poorly documented. In the study here, it was decided to determine if AgNP with a diameter of 70 nm (AgNP70) will alter the biology of human PMN similarly to AgNP20 previously reported to induce apoptosis and inhibit de novo protein synthesis. The results here indicated that, in contrast to AgNP20, AgNP70 delayed PMN apoptosis. However, both AgNP20 and AgNP70 inhibited de novo protein synthesis. Both forms of AgNP did not significantly increase reactive oxygen species (ROS) production, but AgNP20 significantly increased the cell production of the CXCL8 chemokine (IL-8). In addition, AgNP20, but not AgNP70, induced the release of albumin and matrix metalloproteinase-9 (MMP-9/gelatinase B) into culture supernatants. Consistent with this latter observation, gelatinase activity was increased by AgNP20, as assessed by zymography. From these outcomes, it is concluded that two NP with different initial diameters can possess similar - as well as distinct - biological properties in modulating human PMN functions. These outcomes are testimony to the complexity of the modes of action of NP at the cellular level.

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Section: Discussionsupporting

confidence: 93%

Silver nanoparticles of 70 nm and 20 nm affect differently the biology of human neutrophils

Poirier

Simard

Girard

2015

Journal of Immunotoxicology

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“…The degranulation of RBL‐2H3 cells has been studied by different microscopies to show their degranulation processes (Huang et al ., ; Yang et al ., ). But the limited resolution of light microscopy still restricts our understanding of the detailed degranulation processes of RBL‐2H3 cells.…”

Section: Resultsmentioning

confidence: 97%

Atomic force microscopy study of ionomycin‐induced degranulation in RBL‐2H3 cells

Huang

Yang

et al. 2016

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Mast cell degranulation is the typical anaphylaxis process of mast cells associated with the release of cytokines, eicosanoids and their secretory granules, which play very important roles in the allergic inflammatory response of the human body upon anaphylactogen stimulation. The calcium ionophore ionomycin is widely used as a degranulation induction agent for mast cell degranulation studies. In the present work, ionomycin-induced degranulation of RBL-2H3 basophilic leukemia cell line cells was investigated in vitro by high resolution atomic force microscopy (AFM). Ionomycin, which could increase the intracellular free Ca level and β-Hexosaminidase release, was found to induce the formation of a kind of peculiar vesicles in the cytoplasm area of RBL-2H3 cells. Those vesicles induced by ionomycin would desintegrate to release a larger amount of granules surrounding RBL-2H3 cells by the controlling of F-actin. These results provide the precise morphological information of ionomycin-induced mast cell degranulation at nanoscale, which could benefit our understanding of ionomycin-induced mast cell anaphylaxis model and also validate the applicability of AFM for the detection of allergic inflammatory response in mast cells. SCANNING 38:525-534, 2016. © 2016 Wiley Periodicals, Inc.

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“…Furthermore, other studies have observed that the toxicity of silver NPs versus Ag + ions on mammalian cells showed significant toxic effects starting from 200 nM NP concentration [ 35 ], which suggests that our NPs are more toxic. Literature also suggests that other effects on the rat basophilic leukemia cells, such as degranulation, may occur [ 39 ]. We would like to add, that according to the experimental evidence presented in this work the origin of the photoluminescence of silver nanoclusters reported in previous publication [ 40 ] must be glycine dimers, and not the intrinsic emission of the silver clusters, as we suggested before.…”

Section: Resultsmentioning

confidence: 99%

Imaging of Biological Cells Using Luminescent Silver Nanoparticles

Kravets¹,

Almemar²,

Jiang³

et al. 2016

Nanoscale Res Lett

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The application of luminescent silver nanoparticles as imaging agents for neural stem and rat basophilic leukemia cells was demonstrated. The experimental size dependence of the extinction and emission spectra for silver nanoparticles were also studied. The nanoparticles were functionalized with fluorescent glycine dimers. Spectral position of the resonance extinction and photoluminescence emission for particles with average diameters ranging from 9 to 32 nm were examined. As the particle size increased, the spectral peaks for both extinction and the intrinsic emission of silver nanoparticles shifted to the red end of the spectrum. The intrinsic photoluminescence of the particles was orders of magnitude weaker and was spectrally separated from the photoluminescence of the glycine dimer ligands. The spectral position of the ligand emission was independent of the particle size; however, the quantum yield of the nanoparticle-ligand system was size-dependent. This was attributed to the enhancement of the ligand’s emission caused by the local electric field strength’s dependence on the particle size. The maximum quantum yield determined for the nanoparticle-ligand complex was (5.2 ± 0.1) %. The nanoparticles were able to penetrate cell membranes of rat basophilic leukemia and neural stem cells fixed with paraformaldehyde. Additionally, toxicity studies were performed. It was found that towards rat basophilic leukemia cells, luminescent silver nanoparticles had a toxic effect in the silver atom concentration range of 10–100 μM.

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Silver nanoparticle-induced degranulation observed with quantitative phase microscopy

Cited by 3 publications

References 16 publications

Silver nanoparticles of 70 nm and 20 nm affect differently the biology of human neutrophils

Silver nanoparticles of 70 nm and 20 nm affect differently the biology of human neutrophils

Atomic force microscopy study of ionomycin‐induced degranulation in RBL‐2H3 cells

Imaging of Biological Cells Using Luminescent Silver Nanoparticles

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