Silver-nanoparticle exposure on immune system of mice depending on the route of administration

Kalmantaeva, O. V.; Фирстова, В. В.; Потапов, В. Д.; Zyrina, E. V.; Gerasimov, V. N.; Ganina, Elena; Бурмистров, В. А.; Борисов, А. В.

doi:10.1134/s1995078014050061

Cited by 8 publications

(7 citation statements)

References 11 publications

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“…Argovit has already shown to possess a broad spectrum of antimicrobial activity at concentrations that are not toxic for mammalian cells and tissues, for example, immune cells, several organs in mice, and in in vivo experiments in rats. [50][51][52] So the use of AgNPs from Argovit has been approved by international instances as food supplement, cosmetic, and medical devices for veterinary and human use. 20 The genotoxic effects of several NPs have been fully studied, it has been reported that there is a strong consistency between the genotoxic effects of NPs measured by the comet assay with those obtained by the micronucleus assay.…”

Section: Discussionmentioning

confidence: 99%

Comparison of cytotoxicity and genotoxicity effects of silver nanoparticles on human cervix and breast cancer cell lines

et al. 2016

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The wide application of silver nanoparticles (AgNPs) has pointed out the need to evaluate their potential risk and toxic effects on human health. Herein, the cytotoxic effects of Argovit™ AgNPs were evaluated on eight cancer cell lines. Further cytotoxic studies were performed in gynecological cancer cell lines from cervical (HeLa) and breast (MDA-MB-231 and MCF7) cancer. In both cases, the half maximal inhibitory concentration (IC50) of AgNPs produced the formation of reactive oxygen species (ROS) after 24 h of incubation, but it was not statistically significant compared with untreated cells. However, HeLa, MDA-MB-231, and MCF7 cells treated with the maximal IC of AgNPs induced the formation of ROS either at 12 or 24 h of incubation. Genotoxicity achieved by comet assay in HeLa, MDA-MB-231, and MCF7 cells revealed that exposure to IC50 of AgNPs does not induced noticeable DNA damage in the cells. However, the IC of AgNPs provoked severe DNA damage after 12 and 24 h of exposure. We conclude that, Argovit (polyvinylpyrrolidone-coated AgNPs) induce a cytotoxic effect in a time and dose-dependent manner in all the eight cancer cell lines tested. Nevertheless, the genotoxic effect is mainly restricted by the concentration effect. The results contribute to explore new therapeutic applications of AgNPs for malignances in murine models and to study in deep the cytotoxic and genotoxic effects of AgNPs in healthy cells at the surrounding tissue of the neoplasia.

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Section: Discussionmentioning

confidence: 99%

Comparison of cytotoxicity and genotoxicity effects of silver nanoparticles on human cervix and breast cancer cell lines

et al. 2016

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“…Так, малорастворимый фуллерен С60 токсичен при концентрации 0,02 части на миллион, более растворимый С60(ОН)24 был токсичен при концентрациях выше 5000 частей на миллион [46,48]. Однако результаты исследований токсикологической роли растворения НЧ неоднозначны [49,50]. Наибольшее количество работ в этом плане проведено с НЧ серебра.…”

Section: факторы и механизмы повреждающего действия наноматериаловunclassified

“…В проявлении токсичности НЧ существенным может быть ионное высвобождение. НЧ серебра состоят из элементарного серебра, которое не растворимо и не активно в чистой воде, но растворимо в кислых растворах (например, азотной кислоты) [49]. Поверхностное окисление НЧ серебра приводит к образованию высокореакционного ионного серебра, которое как адсорбируется на поверхности НЧ, так и высвобождается в окружающую среду.…”

Section: факторы и механизмы повреждающего действия наноматериаловunclassified

Factors Influencing the Manifestation of Toxicity and Danger of Nanomaterials

Leonenko¹,

Leonenko²

2020

Innov Biosyst Bioeng

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nanoparticles. Most nanoparticles are unstable in dispersion, prone to aggregation and sedimentation, which significantly affects the process of absorption of nanoparticles and their toxicity. Conclusions. The toxicity and danger of nanoparticles and nanomaterials depend on many factors and their combinations. The complexity of assessing the impact of nanostructures is determined by the range of variability of properties, chemical, geometric, physicochemical properties and characteristics, size, surface of nanoparticles. The improvement and development of new approaches to identifying the danger of nanoscale objects is a promising direction of scientific investigations.

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“…Since 2004, Argovit™, a commercial formulation of silver nanoparticles, has been studied in vitro and in vivo for its multiple biomedical applications (antiviral, bactericide, re-epithelialization agent, among others) [ 11 , 12 , 13 , 14 ]. The murine models used thus far do not show toxic effects on immune cells or organs [ 15 ]. This specific AgNP formulation showed cytotoxic effects in eight human cancer cell lines, apparently through the generation of ROS [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

AgNPs Argovit™ Modulates Cyclophosphamide-Induced Genotoxicity on Peripheral Blood Erythrocytes In Vivo

et al. 2021

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Silver nanoparticles (AgNPs) have been studied worldwide for their potential biomedical applications. Specifically, they are proposed as a novel alternative for cancer treatment. However, the determination of their cytotoxic and genotoxic effects continues to limit their application. The commercially available silver nanoparticle Argovit™ has shown antineoplastic, antiviral, antibacterial, and tissue regenerative properties, activities triggered by its capacity to promote the overproduction of reactive oxygen species (ROS). Therefore, in this work, we evaluated the genotoxic and cytotoxic potential of the Argovit™ formulation (average size: 35 nm) on BALB/c mice using the micronucleus in a peripheral blood erythrocytes model. Besides, we evaluated the capability of AgNPs to modulate the genotoxic effect induced by cyclophosphamide (CP) after the administration of the oncologic agent. To achieve this, 5–6-week-old male mice with a mean weight of 20.11 ± 2.38 g were treated with water as negative control (Group 1), an single intraperitoneal dose of CP (50 mg/kg of body weight, Group 2), a daily oral dose of AgNPs (6 mg/kg of weight, Group 3) for three consecutive days, or a combination of these treatment schemes: one day of CP doses (50 mg/kg of body weight) followed by three doses of AgNPs (one dose per day, Group 4) and three alternate doses of CP and AgNPs (six days of exposure, Group 5). Blood samples were taken just before the first administration (0 h) and every 24 h for seven days. Our results show that Argovit™ AgNPs induced no significant cytotoxic or acute genotoxic damage. The observed cumulative genotoxic damage in this model could be caused by the accumulation of AgNPs due to administered consecutive doses. Furthermore, the administration of AgNPs after 24 h of CP seems to have a protective effect on bone marrow and reduces by up to 50% the acute genotoxic damage induced by CP. However, this protection is not enough to counteract several doses of CP. To our knowledge, this is the first time that the exceptional chemoprotective capacity produced by a non-cytotoxic silver nanoparticle formulation against CP genotoxic damage has been reported. These findings raise the possibility of using AgNPs as an adjuvant agent with current treatments, reducing adverse effects.

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Silver-nanoparticle exposure on immune system of mice depending on the route of administration

Cited by 8 publications

References 11 publications

Comparison of cytotoxicity and genotoxicity effects of silver nanoparticles on human cervix and breast cancer cell lines

Comparison of cytotoxicity and genotoxicity effects of silver nanoparticles on human cervix and breast cancer cell lines

Factors Influencing the Manifestation of Toxicity and Danger of Nanomaterials

AgNPs Argovit™ Modulates Cyclophosphamide-Induced Genotoxicity on Peripheral Blood Erythrocytes In Vivo

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