Silver Nanoparticle-Enzyme Composite Films for Hydrogen Peroxide Detection

Abstract

For high-performance biosensor development, two key issues to be significantly considered are the effective integration of biological enzymes with electrode transducer and the rapid communication across the biosensing interface. To combat these issues, the present work reported a robust enzymatic biosensor platform based on binary nanoparticles film composed of enzyme-loaded polymeric nanoparticles and conductive silver nanoparticles (Ag NPs) by a facile electrophoretic deposition method. First, horseradish peroxidase (HRP) as a model enzyme was coassembled with an amphiphilic and photocross-linkable polypeptide of 7-amino-4-methylcoumarin chemically modified poly(γ-glutamic acid) (γ-PGA−AMC, PGA

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

“…Application of ECBs encompasses a wide variety of research area from small biomolecules (dopamine, glucose, xanthine, etc.) to cancer biomarkers and other large biological systems [ 3 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 42 , 44 ]. These biosensors vary from each other based on the use of biorecognition components (BRC) and EAT for the detection process [ 1 , 4 ].…”

“…This is because of enzymes are amino acids that are capable of inducing biochemical catalysis [ 1 , 56 ]. They are capable of interacting with electrochemically inactive bioanalytes and producing electroactive species [ 26 , 27 , 60 ]. Enzymes often significantly increase the rate of reaction and the kinetic parameters can be readily investigated with simple EATs [ 1 , 60 ].…”

“…Besides being highly conducting and biocompatible, AgNPs are also one of the most commonly manufactured MNPs [ 54 , 142 ]. They are more easily oxidized compared to AuNPs in an electrochemical setup [ 19 , 26 , 54 , 143 ]. This makes them an excellent candidate for ECBs.…”

“…Table 1 discusses MNPC-based ECBs that have been reported for the detection of various biomolecules based on the size and shape of MNPs. It can be seen from the table that MNPs with sizes between 5 and 20 nm are the most-used individual component in the ECB fabrication process [ 4 , 23 , 26 , 27 , 29 , 41 , 44 ]. Although size variation is well studied, the shape of the MNPs used is almost always spherical [ 4 , 26 , 41 , 44 , 48 , 85 ].…”

“…Particularly, metal nanoparticles (MNPs) are mostly used due to their biocompatibility, low toxicity, excellent conductivity, and high surface area [ 17 , 18 , 19 , 20 , 21 ]. Among many, gold (AuNPs), silver (AgNPs), and platinum NPs (PtNPs) are some of the most commonly utilized in ECBs [ 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ]. In fabricating biosensors, these MNPs often provide the anchoring site for biorecognition components such as antibodies, enzymes, single stranded RNA (ssRNA) and DNA (ssDNA), aptamers, and affibodies [ 21 , 22 , 23 , 24 , 27 , 29 , 30 , 31 ].…”