Silver Nanocrystals Mediated Combination Therapy of Radiation with Magnetic Hyperthermia on Glioma Cells

Abstract

Based on the study of apoptosis-induced and anti-proliferation behavior of silver nanoparticles (AgNPs) on cancer cells, the attractively therapeutic effect and potential application of AgNPs in anti-cancer field was gradually revealed. Here we investigated the effect of 10 nm silver nanoparticles (AgNPs) on human glioma U251 cells upon the combination treatment of ionizing radiation (IR) treatment with magnetic hyperthermia (MHT). AgNPs showed both radio and thermo sensitivity on U251 cells from the surviving fraction curve. Besides, we found both X-rays and heat could enhance the content of cells uptake of AgNPs. As the amount of intracellular AgNPs accumulated, the apoptosis rate of U251 cells enhanced. Furthermore, we established a simplified model for calculating cell survival rate and demonstrated that after RT, MHT and RT combined with MHT, AgNPs could significantly inhibited cancer cell proliferation. Our results revealed that AgNPs could have a potential application in enhancing effect of RT with MHT combination therapy induced killing of cancer cells.

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

“…68 Jiang et al reported that cellular uptake of AgNPs of #20 nm was greater than that of AgNPs of .100 nm in human glioma U251 cells. 69 Our results are consistent with those previously reported. For example, Park et al showed the effects of various sizes of AgNPs (20, 80, and 113 nm) using in vitro assays for cytotoxicity, inflammation, genotoxicity, and developmental toxicity, and finally concluded that AgNPs of 20 nm were more toxic than larger nanoparticles.…”

Magnetic hyperthermia therapy for the treatment of glioblastoma: a review of the therapy’s history, efficacy and application in humans

“…Combination of MHT with chemotherapy and/or RT has already been studied to a limited extent [106,107]. Phase I and II studies of MHT in combination with RT have been performed in patients with recurrent GBM (see below).…”

“…In another study, U251 human glioma cells were initially treated in vitro with silver nanocrystals (AgNPs), maghemite MNPs or a combination of both, followed by RT and AMF treatment [107]. All treatment groups received 0–6 Gy of RT and were then exposed to an AMF for 15 min.…”

Nanoparticle-Mediated Combination Therapy: Two-in-One Approach for Cancer

“…As intracellular AgNPs accumulated, the apoptosis rate of U251 cells was enhanced. Furthermore, AgNPs significantly inhibited cancer cell proliferation when combined with hyperthermic conditions [ 187 ]. The combination of AgNPs and DOX could form stable complexes that strongly interacted with DNA.…”