Silver Nanoclusters Beacon as Stimuli-Responsive Versatile Platform for Multiplex DNAs Detection and Aptamer–Substrate Complexes Sensing

Liu, Guoliang; Li, Jingjing; Feng, Da-Qian; Zhu, Jun‐Jie; Wang, Wei

doi:10.1021/acs.analchem.6b04362

Cited by 97 publications

(54 citation statements)

References 39 publications

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“…Not only inorganic analytes can be sensed with silver nanoclusters, but also the detection of biomolecules has been explored by several groups. Among examples of biomolecular detection are gene detection [23], protein detection [24][25][26], detection of specific nucleic acid sequences [27][28][29], and cellular labeling [30,31]. Recently, advanced detection procedures have also been incorporated with the use of AgNCs such as the multiplexed sensing capability of several genes [32] or surface plasmon enhanced energy transfer (SPEET) between AgNCs and AuNP for protein [33] or DNA [34] detection.…”

Section: Introductionmentioning

confidence: 99%

Micro RNA Sensing with Green Emitting Silver Nanoclusters

Yourston

Krasnoslobodtsev

2020

Molecules

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Micro RNA (miR) are regulatory non-coding RNA molecules, which contain a small number of nucleotides ~18–28 nt. There are many various miR sequences found in plants and animals that perform important functions in developmental, metabolic, and disease processes. miRs can bind to complementary sequences within mRNA molecules thus silencing mRNA. Other functions include cardiovascular and neural development, stem cell differentiation, apoptosis, and tumors. In tumors, some miRs can function as oncogenes, others as tumor suppressors. Levels of certain miR molecules reflect cellular events, both normal and pathological. Therefore, miR molecules can be used as biomarkers for disease diagnosis and prognosis. One of these promising molecules is miR-21, which can serve as a biomarker with high potential for early diagnosis of various types of cancer. Here, we present a novel design of miR detection and demonstrate its efficacy on miR-21. The design employs emissive properties of DNA-silver nanoclusters (DNA/AgNC). The detection probe is designed as a hairpin DNA structure with one side of the stem complimentary to miR molecule. The binding of target miR-21 opens the hairpin structure, dramatically modulating emissive properties of AgNC hosted by the C12 loop of the hairpin. “Red” fluorescence of the DNA/AgNC probe is diminished in the presence of the target miR. At the same time, “green” fluorescence is activated and its intensity increases several-fold. The increase in intensity of “green” fluorescence is strong enough to detect the presence of miR-21. The intensity change follows the concentration dependence of the target miR present in a sample, which provides the basis of developing a new, simple probe for miR detection. The detection strategy is specific, as demonstrated using the response of the DNA/AgNC probe towards the scrambled miR-21 sequence and miR-25 molecule. Additionally, the design reported here is very sensitive with an estimated detection limit at ~1 picomole of miR-21.

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Section: Introductionmentioning

confidence: 99%

Micro RNA Sensing with Green Emitting Silver Nanoclusters

Yourston

Krasnoslobodtsev

2020

Molecules

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“…While the synthetic tolerances are more demanding, success in generating nanoparticles with diameters approximating Fermi wavelengths generates metal nanoparticles that exhibit discrete energy levels mirroring molecular orbital transitions . By relying on sequence‐dependent assembly of small‐scale nanoclusters, Liu et al developed the first “activatable silver nanoclusters beacon (ASNCB)” probe that contained variations of a 5′ DNA scaffold sequence, allowing assembly of silver nanoparticles into three discrete nanocrystals (Ag NCs) with unique emission properties . In the case of the aptamer complexes, ATP and thrombin were the proof‐of‐concept targets.…”

Section: Design and Application Of Mab Constructsmentioning

confidence: 99%

Molecular Aptamer Beacons and Their Applications in Sensing, Imaging, and Diagnostics

Moutsiopoulou

Broyles

Dikici

et al. 2019

Small

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The ability to monitor types, concentrations, and activities of different biomolecules is essential to obtain information about the molecular processes within cells. Successful monitoring requires a sensitive and selective tool that can respond to these molecular changes. Molecular aptamer beacon (MAB) is a molecular imaging and detection tool that enables visualization of small or large molecules by combining the selectivity and sensitivity of molecular beacon and aptamer technologies. MAB design leverages structure switching and specific recognition to yield an optical on/off switch in the presence of the target. Various donor–quencher pairs such as fluorescent dyes, quantum dots, carbon‐based materials, and metallic nanoparticles have been employed in the design of MABs. In this work, the diverse biomedical applications of MAB technology are focused on. Different conjugation strategies for the energy donor–acceptor pairs are addressed, and the overall sensitivities of each detection system are discussed. The future potential of this technology in the fields of biomedical research and diagnostics is also highlighted.

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“…In addition to aptamer–gold nanomaterials for in vivo applications, silver nanomaterials have also been used, among which nucleic acid–stabilized silver nanoclusters (Ag NCs) are the most attractive. Ag NCs possess good fluorescence properties, excellent photostability, sub‐nanometer size, as well as low cytotoxicity.…”

Section: Molecular Engineering Of Aptamer‐based Nanomaterials Toward mentioning

confidence: 99%

“…Due to its absence in biological systems, L‐DNA is resistant to nuclease digestion and hence has resulted in NCs with much higher intracellular stability than those templated by regular DNA, thus making cell‐type‐specific imaging possible at physiological conditions, with much lower Ag NC concentration than D‐DNA‐templated ones. Recently, Liu et al synthesized target‐responsive Ag NC beacon (ASNCB) for multiplex DNAs, small molecule, and protein sensing using a one‐pot synthesis . Incorporating an ATP aptamer to trigger ATP‐responsive structure transformation of ASNCB, ATP binding event induced ASNCB conformational transition and increased fluorescent signal of silver nanoclusters.…”

Section: Molecular Engineering Of Aptamer‐based Nanomaterials Toward mentioning

confidence: 99%

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Zhang

Lan

2019

Adv Healthcare Materials

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equally exciting and perhaps more challenging field of application is toward their in vivo applications, including living animals and human bodies, in diverse areas, [2] such as sensing, drug delivery, medical imaging, and cancer therapy. However, most of these nanomaterials lack selectivity toward targets of interests. Therefore, to employ these nanomaterials for a wider range of in vivo applications, various molecular engineering approaches have been employed to obtained nanomaterials functionalized with biomolecules to enable selective targeting. [3][4][5] Among these biomolecules, functional nucleic acids, or FNAs, have shown the most promise.FNAs are DNA or RNA molecules that can interact with or bind to a specific analyte, resulting in a conformational change and/or a catalytic reaction. [6] As their names suggested, ribozymes and deoxyribozymes (called DNAzyme hereafter) can act as enzymes in the presence of cofactors to catalyze various reactions, including cleavage and ligation of RNA/DNA, and phosphorylation and peroxidation of DNA. On the other hand, riboswitches and DNA aptamers are antibody-like receptors that can bind target molecules with high affinity and selectivity. Furthermore, the binding abilities of riboswitches and aptamers can be combined with the enzymatic function of ribozymes or DNAzymes to form aptazymes so that the binding of targets can inhibit or enhance the catalytic activities. These nucleic acids, including DNAzymes, aptamers, and aptazymes, are collectively known as FNAs to emphasize their functions beyond the traditional genetic storage and transformations roles for DNA and RNA. Unlike DNA and RNA inside biological systems, FNAs are isolated via a combinatorial technique known as in vitro selection, or a process also known as systematic evolution of ligands by exponential enrichment (SELEX). The discovery of new functions of nucleic acids has not only resulted in major advances in the fields of molecular biology and biochemistry, but also revolutionized sensors designs for both in vitro and in vivo applications. [7,8] Over the past decade, numerous FNA-based nanomaterials have been developed. [9][10][11][12] Among these FNA-based nanosystems, the functions of FNAs can be categorized into two types: diagnostic function and therapeutic function. For the diagnostic function, the FNAs serve either as the biorecognition ligands for direct sensing and imaging of the Recent advances in nanotechnology and engineering have generated many nanomaterials with unique physical and chemical properties. Over the past decade, numerous nanomaterials are introduced into many research areas, such as sensors for environmental monitoring, food safety, point-ofcare diagnostics, and as transducers for solar energy transfer. Meanwhile, functional nucleic acids (FNAs), including nucleic acid enzymes, aptamers, and aptazymes, have attracted major attention from the biomedical community due to their unique target recognition and catalytic properties. Benefiting from the recent progress of molecular engine...

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Silver Nanoclusters Beacon as Stimuli-Responsive Versatile Platform for Multiplex DNAs Detection and Aptamer–Substrate Complexes Sensing

Cited by 97 publications

References 39 publications

Micro RNA Sensing with Green Emitting Silver Nanoclusters

Micro RNA Sensing with Green Emitting Silver Nanoclusters

Molecular Aptamer Beacons and Their Applications in Sensing, Imaging, and Diagnostics

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

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