Silver Nano Particles Prevent Platelet Adhesion on Immobilized Fibrinogen

Abstract

Vascular thrombotic disorders have emerged as a serious threat to our society. Platelet adhesion to fibrinogen, collagen and other platelet activators exposed over the atherosclerotic plaques can trigger platelet signaling events, activate platelets and lead to thrombotic events. Since anticoagulant and thrombolytic treatment strategies are usually associated with serious bleeding complications, preventing platelets adhesion may help to maintain platelets in an inactive state. In this study we tried to find out the effect of Silver nanoparticles, through their interaction with various platelet surface integrins on platelet adhesion on immobilized fibrinogen. Platelets, isolated from anticoagulated human whole blood sample from healthy donors, were suspended in physiological buffer and each sample was divided into four tubes. In three of them 0.05, 0.5, and 5 lM concentrations of Silver nanoparticles were added, fourth tube served as control. Platelet adhesion on immobilized fibrinogen matrices and integrin mediated cell signaling events were studied in all the four samples. In the present study we show that nanosilver prevent platelet adhesion without conferring any lytic effect on them and effectively prevents integrin-mediated platelet responses in a concentration-dependent manner.

An Evaluation of Blood Compatibility of Silver Nanoparticles

“…Coagulation time for the three main pathways which are intrinsic, extrinsic and final common pathways can be evaluated by activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT), respectively. It has been known that the normal physiological levels for APTT, PT and TT were 25.1~36.5 s, 9.4~12.5 s, 10.3~16.6 s, respectively 26 27 . After treated with different concentrations of AgNP-PVP-20 ( Fig.…”

“…In this study, we have systematically investigated hemolysis, platelet aggregation, coagulation, lymphocyte proliferation and the activation of complement system after the treatment with 20 nm AgNPs coated with polyvinyl pyrrolidone (AgNP-PVP-20) and citrate (AgNP-CIT-20). Since plasma corona of nanoparticles has implications on biological processes that control haemostasis, thrombosis and inflammatory responses 26 27 28 29 30 . AgNPs coronas were characterized by label-free quantitative mass spectrometry analysis.…”

The silver lining: towards the responsible and limited usage of silver

“…In another study, nanosilver prevented platelet adhesion without conferring any lytic effect on them and effectively prevented integrin‐mediated platelet responses in a concentration‐dependent manner (Bandyopadhyay et al . ). Antiplatelet activity has also been demonstrated by Gluconobacter roseus ‐mediated biologically synthesized AgNPs (Krishnaraj and Berchmans ).…”

Bio‐effect of nanoparticles in the cardiovascular system

“…Platelet activation triggers of platelet signaling events and thrombotic events when the platelets adhere to fibrinogen (Fbg), collagen, and other platelet activators on the atherosclerotic plaques. Bandyopadhyay et al demonstrated that Ag NPs inhibited platelet adhesion, without lysing the cells, and effectively prevented the integrin‐mediated platelet responses in a dose‐dependent manner.…”