Silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus

Panferov, Vasily G.; Safenkova, Irina V.; Byzova, Nadezhda A.; Varitsev, Yuri A.; Zherdev, Anatoly V.; Dzantiev, Boris B.

doi:10.1080/09540105.2017.1401044

Cited by 53 publications

(23 citation statements)

References 53 publications

(54 reference statements)

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“…The application of nanoparticles in new technologies used in non-laboratory rapid screening methods for the detection of plant pathogens has a significant impact on the quality of agricultural products. In a study by Panferov et al [ 97 ], an enhanced and rapid method based on lateral flow immunoassay (LFIA) was developed to detect low levels of potato leaf roll virus (PLRV) in contaminated fields. In this method, AuNPs were used as labels and silver ions were reduced at the AuNP surface to increase sensitivity [ 97 ].…”

Section: Application Of Green Synthesized Mtnps In Agriculturementioning

confidence: 99%

“…In a study by Panferov et al [ 97 ], an enhanced and rapid method based on lateral flow immunoassay (LFIA) was developed to detect low levels of potato leaf roll virus (PLRV) in contaminated fields. In this method, AuNPs were used as labels and silver ions were reduced at the AuNP surface to increase sensitivity [ 97 ]. In another report, infection of potato tubers with Ralstonia solanacearum was detected using an AuNP-based immunoassay.…”

Section: Application Of Green Synthesized Mtnps In Agriculturementioning

confidence: 99%

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Green synthesis of metal nanoparticles using microorganisms and their application in the agrifood sector

et al. 2021

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The agricultural sector is currently facing many global challenges, such as climate change, and environmental problems such as the release of pesticides and fertilizers, which will be exacerbated in the face of population growth and food shortages. Therefore, the need to change traditional farming methods and replace them with new technologies is essential, and the application of nanotechnology, especially green technology offers considerable promise in alleviating these problems. Nanotechnology has led to changes and advances in many technologies and has the potential to transform various fields of the agricultural sector, including biosensors, pesticides, fertilizers, food packaging and other areas of the agricultural industry. Due to their unique properties, nanomaterials are considered as suitable carriers for stabilizing fertilizers and pesticides, as well as facilitating controlled nutrient transfer and increasing crop protection. The production of nanoparticles by physical and chemical methods requires the use of hazardous materials, advanced equipment, and has a negative impact on the environment. Thus, over the last decade, research activities in the context of nanotechnology have shifted towards environmentally friendly and economically viable ‘green’ synthesis to support the increasing use of nanoparticles in various industries. Green synthesis, as part of bio-inspired protocols, provides reliable and sustainable methods for the biosynthesis of nanoparticles by a wide range of microorganisms rather than current synthetic processes. Therefore, this field is developing rapidly and new methods in this field are constantly being invented to improve the properties of nanoparticles. In this review, we consider the latest advances and innovations in the production of metal nanoparticles using green synthesis by different groups of microorganisms and the application of these nanoparticles in various agricultural sectors to achieve food security, improve crop production and reduce the use of pesticides. In addition, the mechanism of synthesis of metal nanoparticles by different microorganisms and their advantages and disadvantages compared to other common methods are presented.

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Section: Application Of Green Synthesized Mtnps In Agriculturementioning

confidence: 99%

Section: Application Of Green Synthesized Mtnps In Agriculturementioning

confidence: 99%

Green synthesis of metal nanoparticles using microorganisms and their application in the agrifood sector

et al. 2021

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“…Sandwich ELISA was carried out according to Panferov et al [ 33 ] with the following modifications: the captured antibodies were used at a concentration of 1 μg∙mL −1 in PBS; the viruses were at concentrations ranging from 0.45 ng∙mL −1 to 1000 ng∙mL −1 ; the biotinylated antibodies were at a concentration of 1 μg∙mL −1 . After the completion of all of the stages, including the addition of streptavidin–polyperoxidase conjugate, TMB substrate, and stop solution (1 M H 2 SO 4 ), the optical density at 450 nm (OD 450 ) was measured.…”

Section: Methodsmentioning

confidence: 99%

How to Improve Sensitivity of Sandwich Lateral Flow Immunoassay for Corpuscular Antigens on the Example of Potato Virus Y?

Razo

Panferov

Safenkova

et al. 2018

Sensors

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A simple approach was proposed to decrease the detection limit of sandwich lateral flow immunoassay (LFIA) by changing the conditions for binding between a polyvalent antigen and a conjugate of gold nanoparticles (GNPs) with antibodies. In this study, the potato virus Y (PVY) was used as the polyvalent antigen, which affects economically important plants in the Solanaceae family. The obtained polyclonal antibodies that are specific to PVY were characterized using a sandwich enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR). For LFIA, the antibodies were conjugated with GNPs with a diameter of 17.4 ± 1.0 nm. We conducted LFIAs using GNP conjugates in a dried state on the test strip and after pre-incubation with a sample. Pre-incubating the GNP conjugates and sample for 30 s was found to decrease the detection limit by 60-fold from 330 ng∙mL−1 to 5.4 ng∙mL−1 in comparison with conventional LFIA. The developed method was successfully tested for its ability to detect PVY in infected and uninfected potato leaves. The quantitative results of the proposed LFIA with pre-incubation were confirmed by ELISA, and resulted in a correlation coefficient of 0.891. The proposed approach is rapid, simple, and preserves the main advantages of LFIA as a non-laboratory diagnostic method.

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“…The layered build-up of gold nanoparticles was described by Li et al [132]. Anfossi et al [133] and Panferov et al [134,135] considered the possibilities of silver enhancement (restoration of the silver salt on the surface of a gold nanoparticle with an increase in its size) in LFIA. In a study by Rodriguez et al [136], the optimal regimes of silver and gold enhancements were determined to enhance the signal from the gold nanoparticles.…”

Section: Proper Response For Lfiamentioning

confidence: 99%

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Zherdev¹,

Dzantiev²

2018

Rapid Test - Advances in Design, Format and Diagnostic Applications

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This chapter considers factors influencing sensitivity of lateral flow immunoassay and modern developments that are focused on reaching lower detection limits. The existing variety of proposed approaches is classified in accordance with the "big five rules" for these assays, including proper sample, receptor, interaction, response, and output. The solutions for rapid extraction of target analytes and preventing negative influence of extractants are considered. Role to antibodies affinity and specificity is characterized. Potential of alternate bioreceptor molecules is discussed. Immunoreactants' compositions, concentrations, and locations on the test strip are characterized as factors determining assay parameters. The existing variety of labels is compared in terms of their optical and alternate registration. Tools to modulate a sequence of analytical reactions and to form aggregates of the detected labels are considered. The discussed approaches are illustrated through developments of test strips for detection of mycotoxins, veterinary drugs, and other analytes.The overall design of the immunochromatographic test strip is shown in Figure 1. It is a composite of several membranes of different structures and porosities, fixed on a support. The bundling of the test strip can vary, so it makes sense to consider its design based on what analytical tasks are being performed on its different sites.A. Typically, the lower portion of the test strip contains a sample pad. It ensures the absorption of sample components, in which the presence of the target analyte is checked.B. The following is a section with immunoreagents that are washed out during the analysis and move upward along with the components of the sample. As a rule, a conjugate pad forms this zone. It contains a conjugate of antibodies against the target analyte with a nanodispersed label-particles of colored latex, colloidal gold, and so on.The next two sections are located on the main working membrane of the test strip. C.First, there is a zone along which the movement of the absorbed components of the sample and the washed immunoreagents continues. During this movement, immune reactions occur, and specific intermolecular complexes are formed. D.Next, a mixture of reacted and unreacted molecules enters the binding area with immobilized immunoreagents. Depending on whether the target analyte was present in the sample and in what amount, binding of labeled immune complexes occurs in certain areas (in the traditional case, with the formation of narrow colored lines). Usually, additional reagents are located here to control the functionality of the test system. E. The upper part of the test strip with the final pad, usually structurally similar to the sample pad, ensures the further movement of the reaction mixture under the action of capillary forces and the washing of unreacted components from the underlying areas. These processes allow the label's binding to be evaluated correctly.Membrane components of the test strip are fixed on a plastic support and partia...

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Silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus

Cited by 53 publications

References 53 publications

Green synthesis of metal nanoparticles using microorganisms and their application in the agrifood sector

Green synthesis of metal nanoparticles using microorganisms and their application in the agrifood sector

How to Improve Sensitivity of Sandwich Lateral Flow Immunoassay for Corpuscular Antigens on the Example of Potato Virus Y?

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

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