Silver Doped Mesoporous Silica Nanoparticles Based Electrochemical Enzyme-Less Sensor for Determination of H2O2 Released from Live Cells

Abstract

In this study, a silver doped mesoporous silica nanoparticles-based enzyme-less electrochemical sensor for the determination of hydrogen peroxide (H2O2) released from live cells was constructed for the first time. The presented electrochemical sensor exhibited fast response (2 s) towards the reduction of H2O2 concentration variation at an optimized potential of −0.5 V with high selectivity over biological interferents such as uric acid, ascorbic acid, and glucose. In addition, a wide linear range (4 μM to 10 mM) with a low detection limit (LOD) of 3 μM was obtained. Furthermore, the Ag-mSiO2 nanoparticles/glass carbon electrode (Ag-mSiO2 NPs/GCE) based enzyme-less sensor showed good electrocatalytic performance, as well as good reproducibility, and long-term stability, which provided a successful way to in situ determine H2O2 released from live cells. It may also be promising to monitor the effect of reactive oxygen species (ROS) production in bacteria against oxidants and antibiotics.

Synergistic Antibacterial Activity of Silver-Loaded Graphene Oxide towards Staphylococcus Aureus and Escherichia Coli

“…This method has been associated with cell cycle arrest, apoptosis, migration, and inflammation and antibacterial activity. Thereby, the CV test of H 2 O 2 plays an important role in identifying the biological and pathological mechanism systems [51]. According to Figure 4b, no peak was detected in the GO sample, whereas a small reduction peak near −0.1 mV was found in Ag NPs, indicating that Ag NPs have a potential ROS response [52].…”

Electrodeposition of Silver Nanoparticles on Indium-Doped Tin Oxide Using Hydrogel Electrolyte for Hydrogen Peroxide Sensing

“…Moreover, the catalytic activity and selectivity for H 2 O 2 are significantly reduced by other interfering substances with similar detection potentials, such as dopamine, uric acid, catecholamine, and ascorbic acid. Because of these disadvantages of enzyme-based electrochemical sensing for H 2 O 2 , metal nanoparticles are prospectively good alternatives for replacing enzyme transducers owing to their outstanding conductivity, long-term stability, and excellent electrocatalytic activity [ 41 , 44 ].…”

Polyethyleneimine-assisted formation of Ag–SiO2 hybrid microspheres for H2O2 sensing and SERS applications

“…The lower sensitivity and good limit of detection for the Ag-SiO 2 microspheres implied a good catalytic ability for H 2 O 2 reduction. 67,68 Further, the selectivity of the electrochemical sensor for the detection of H 2 O 2 was studied by carrying out a series of CV measurements in the presence of varying interfering agents, such as glucose, ethanol, and ascorbic acid, and are depicted in Fig. S8a in the ESI.…”

“…The lower sensitivity and good limit of detection for the Ag–SiO 2 microspheres implied a good catalytic ability for H 2 O 2 reduction. 67,68…”