Silver decahedral nanoparticles empowered SPR imaging-SELEX for high throughput screening of aptamers with real-time assessment

Jia, Wenchao; Li, Hui; Wilkop, Thomas; Liu, Xiao‐Hui; Yu, Xiao-Dong; Cheng, Quan; Xu, Danke; Chen, Hong‐Yuan

doi:10.1016/j.bios.2018.02.029

Cited by 30 publications

(12 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Surface plasmon resonance imaging (SPRi) is one of the state of the art methods that provides real time, label‐free determination of binding kinetics in a high‐throughput manner. While SPRi may be already used during the selection phase the post synthetic use of high purity aptamers for the assessment of target binding kinetics is more common because the sequence fidelity of aptamers is even more critical than when nucleic acids are used for hybridization assays . Moreover, SPRi could be used successfully also to investigate in real‐time the intricacy of aptamer sandwich complex formation .…”

Section: Introductionmentioning

confidence: 99%

Finding the Optimal Surface Density of Aptamer Monolayers by SPR Imaging Detection‐based Aptamer Microarrays

2020

View full text Add to dashboard Cite

Surface plasmon resonance imaging (SPRi) by enabling label‐free, real time assessment of biomolecular interactions in multiplexed manner is one of the methods of choice for high throughput characterization of large pools of DNA aptamer candidates following in vitro selection. Moreover, with major advances in in situ amplification methods SPRi became also a viable detection platform for aptamer microarrays. In case of aptamer microarrays, commonly prepared by microspotting, the direct assessment of the surface density of aptamer probes, which is essential for both kinetic and sensing measurements is not possible. Therefore, here we introduce a methodology for simple, one‐step determination of surface densities of thiol labelled aptamer monolayers microspotted on gold SPRi chips. Based on this methodology we investigated in detail the effect of the surface density of aptamers on target binding through two aptamer‐target systems, i. e. human immunoglobulin E (hIgE) and six histidine tag 6xHis‐tag. We found that the surface density of the aptamers is indeed critical and shows a sharp maximum in terms of target binding efficiency, which is largely determined by the size of the target. The optimal aptamer surface densities determined, the immobilization chemistry (shared by many detection platforms, e. g., electrochemical, surface acoustic) and the trends identified may be used for rapid rational optimization of aptamer‐target assays.

show abstract

Section: Introductionmentioning

confidence: 99%

Finding the Optimal Surface Density of Aptamer Monolayers by SPR Imaging Detection‐based Aptamer Microarrays

2020

View full text Add to dashboard Cite

show abstract

“…The first one is the affinity estimation of the oligonucleotide pool selected in each round. For this purpose, the direct measurement of the binding/dissociation constant of the pool can be performed using methods such as surface plasmon resonance (SPR) [126,127], enzyme-linked oligonucleotide assay (ELONA) [90], fluorescent dye-linked aptamer assay (FLAA) [128], and many others, which mostly depend on the specific target and the intended use of the aptamer. In this case, the selection is terminated when the bulk affinity of the pool has stopped increasing or has reached a desired value.…”

Section: Monitoring Of Selection Progress and Selection Cycle Numbermentioning

confidence: 99%

Inside the Black Box: What Makes SELEX Better?

2019

View full text Add to dashboard Cite

Aptamers are small oligonucleotides that are capable of binding specifically to a target, with impressive potential for analysis, diagnostics, and therapeutics applications. Aptamers are isolated from large nucleic acid combinatorial libraries using an iterative selection process called SELEX (Systematic Evolution of Ligands by EXponential enrichment). Since being implemented 30 years ago, the SELEX protocol has undergone many modifications and improvements, but it remains a laborious, time-consuming, and costly method, and the results are not always successful. Each step in the aptamer selection protocol can influence its results. This review discusses key technical points of the SELEX procedure and their influence on the outcome of aptamer selection.

show abstract

“…Silver nanoparticles (AgNPs) have also been used for SELEX ( Figure 3 C) [ 80 ]. AgNPs exhibit strong plasmon resonance and have therefore been used extensively to label molecules using various surface plasmon resonance (SPR)-based techniques [ 99 , 100 ].…”

Section: Current Aptamer Discovery Strategies To Detect Chemicals and Biomoleculesmentioning

confidence: 99%

“…AgNPs exhibit strong plasmon resonance and have therefore been used extensively to label molecules using various surface plasmon resonance (SPR)-based techniques [ 99 , 100 ]. Using this property of AgNPs, AgNP-based SPR imaging (SPRi)-SELEX has been developed and used to select an aptamer for lactoferrin (Lac) [ 80 ]. An ssDNA library was attached to AgNPs with decahedral structures.…”

Section: Current Aptamer Discovery Strategies To Detect Chemicals and Biomoleculesmentioning

confidence: 99%

Recent Advances in Micro/Nanomaterial-Based Aptamer Selection Strategies

Kim

Lee

et al. 2021

Molecules

View full text Add to dashboard Cite

Aptamers are artificial nucleic acid ligands that have been employed in various fundamental studies and applications, such as biological analyses, disease diagnostics, targeted therapeutics, and environmental pollutant detection. This review focuses on the recent advances in aptamer discovery strategies that have been used to detect various chemicals and biomolecules. Recent examples of the strategies discussed here are based on the classification of these micro/nanomaterial-mediated systematic evolution of ligands by exponential enrichment (SELEX) platforms into three categories: bead-mediated, carbon-based nanomaterial-mediated, and other nanoparticle-mediated strategies. In addition to describing the advantages and limitations of the aforementioned strategies, this review discusses potential strategies to develop high-performance aptamers.

show abstract

Silver decahedral nanoparticles empowered SPR imaging-SELEX for high throughput screening of aptamers with real-time assessment

Cited by 30 publications

References 35 publications

Finding the Optimal Surface Density of Aptamer Monolayers by SPR Imaging Detection‐based Aptamer Microarrays

Finding the Optimal Surface Density of Aptamer Monolayers by SPR Imaging Detection‐based Aptamer Microarrays

Inside the Black Box: What Makes SELEX Better?

Recent Advances in Micro/Nanomaterial-Based Aptamer Selection Strategies

Contact Info

Product

Resources

About