Silver catfish (Rhamdia quelen) breeders fed on crude glycerin-containing diets exhibited metabolic alterations and increased sperm concentration

Bombardelli, Robie Allan; Oliveira, Eder José de; Syperreck, Mirna Adriane; Pedreira, Ahiana Cássia de Oliveira; Freitas, Jakeline Marcela Azambuja de; Marques, Antônio Ernesto Meister Luz; Cestari, Marta Margarete; Meurer, Fábio

doi:10.1016/j.aquaculture.2020.735724

Cited by 9 publications

(3 citation statements)

References 61 publications

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“…Rhamdia quelen, commonly known as the silver catfish or jundia, is a neotropical catfish highly valued as a protein source for human consumption and aquaculture in southern Brazil, Argentina, and Uruguay [27][28][29][30]. With its increasing significance, extensive research has focused on feeding management for growth [31,32], breeder nutrition [33][34][35], and the reproductive processes, encompassing sperm quality and gamete conservation [19,[36][37][38][39][40][41]. However, the literature lacks comprehensive coverage of several reproductive parameters, especially those related to testis function, including cryopreservation, transplantation, and spermatogenesis [18,19,23,39].…”

Section: Introductionmentioning

confidence: 99%

Successful Cryopreservation of Spermatogonia Stem Cells of Neotropical Catfish (Rhamdia quelen) and Enriched Germ Cell Transplantation into Common Carp (Cyprinus carpio) Testes

Rosa,

Martinez,

Digmayer

et al. 2023

Fishes

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Cryopreservation and transplantation of spermatogonial stem cells (SSCs) offer new possibilities in the conservation of valuable genetic resources. Therefore, the present study developed a cryopreservation method for whole testicular tissue and for spermatogonial stem cells of jundia catfish (Rhamdia quelen) and developed an enriched germ cell transplantation of jundia catfish into depleted common carp (Cyprinus carpio) testes. Our findings from whole testes indicate that the cryoprotectants MeOH (1.3 M), DMSO (1.4 M), and EG (1.4 M) resulted in high cell viability rates of 67%, 62%, and 51.5%, respectively. Notably, in the case of enriched post-thaw SSCs, DMSO exhibited the highest cell viability at 27%, followed by EG at 16% and MeOH at 7%. Additionally, we observed the successful colonization and proliferation of jundia germ cells within the recipient gonads of common carp following transplantation. Notably, Sertoli cells were identified in the recipient gonads, providing support to the stained donor germ cells and indicated the formation of cysts. Our data suggest that cryopreserving entire testicular tissue presents a viable alternative to cryopreserving isolated testicular cells, and the spermatogonial cells isolated from testes of jundia retained transplantability characteristics. Nonetheless, more investigations are required to reach the goal of functional gamete and to assess the effectiveness of transplantation using these cryopreserved tissues. Taken together, proper cryopreservation methodology and transplantation technology could aid the preservation practice of fish genetic resources.

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Section: Introductionmentioning

confidence: 99%

Successful Cryopreservation of Spermatogonia Stem Cells of Neotropical Catfish (Rhamdia quelen) and Enriched Germ Cell Transplantation into Common Carp (Cyprinus carpio) Testes

Rosa,

Martinez,

Digmayer

et al. 2023

Fishes

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“…No adverse effects were observed from the replacement of maize by crude glycerol on the performance and survival of male reproductive silver catfish (25 to 200 g) (Bombardelli et al, 2020). Theisen et al (2019) state that crude glycerol can be added to the diet of R. voulezi and R. branneri post‐larvae up to 8.8% without affecting performance, can be used as an alternative dietary ingredient for Rhamdia genus fish, resulting in decreased feeding costs.…”

Section: Discussionmentioning

confidence: 99%

Biodiesel‐derived glycerol as a dietary maize replacement for silver catfish ( Rhamdia quelen ) during the fattening phase

Mauerwerk

Zadinelo

Dias

et al. 2022

Aquaculture Research

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Brazilian agriculture has undergone important changes since the 1960s, where public policies were responsible for building an institutional environment favourable to innovation and knowledge and technology adaptation (Vieira Filho & Fishlow, 2017). This has, in turn, led to dramatic increases in agricultural production (Schulter & Filho, 2017).Food security concerns and a growing interest in environmental sustainability are among the main challenges to be faced by countries in the coming decades, due to worldwide population growths (Schulter & Filho, 2017;Vieira Filho & Fishlow, 2017). The productive efficiency of agricultural activities, however, still requires improvements, driven by exponential food demand increases by the world's population (Food and Agriculture Organization of the United Nations [FAO], 2017). In this context, aquaculture has contributed effectively to animal production worldwide, displaying the greatest development in recent years among agricultural activities (FAO, 2020).It is estimated that the global fish production reached 179 million tons in 2018, of which 156 million were destined for human

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“…In the liver´s intracellular compartment, oxidation of glycerol can occur to produce energy through glycolysis and the Krebs cycle (Santos, L. et al, 2019;Silva et al, 2014). Studies demonstrated that as an energy source, glycerin can be included on feeding, thus decreasing the cost of feed for ruminants (Costa et al, 2020), swines (Martínez-Miró et al, 2021), birds (Avellaneda et al, 2020) and more recently for fish (Bombardelli et al, 2021b;Matos et al, 2016;Theisen et al, 2020). However, most studies focused on the apparent effects, such as the animal's zootechnical performance.…”

Section: Introductionmentioning

confidence: 99%

Crude glycerin in feed for Colossoma macropomum fish: evaluation of histopathological effects on the liver and kidney

Santos,

Moron

2023

BIP

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The present study aimed to verify whether the use of crude glycerin in the diet of juvenile Colossoma macropomum could promote histopathological changes in the liver and kidneys. 150 juveniles were used, divided into 15 tanks of 1000 liters (five treatments and three repetitions, n = 10). Five different diets with inclusion of crude glycerin (0%; 7.5%; 10%; 12.5%; 15%) were used in exchange for soybean oil and partial replacement of corn bran. The animals were fed to satiation for 60 days. After this period, liver and kidney samples from each group were submitted to histological processing and histopathological analysis. The semi-quantitative method was used to assess the lesions: calculation of Mean Alteration Values (MVA), which assess the occurrence of the lesion, and the Histopathological Alterations Index (HAI), which allows assessing the severity of the lesions. The results indicated significant non-harmful effects, but adaptive responses to the diet. In conclusion, the inclusion of up to 15% crude glycerin can be used without causing significant liver and kidney changes in C. macropomum juveniles.

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Silver catfish (Rhamdia quelen) breeders fed on crude glycerin-containing diets exhibited metabolic alterations and increased sperm concentration

Cited by 9 publications

References 61 publications

Successful Cryopreservation of Spermatogonia Stem Cells of Neotropical Catfish (Rhamdia quelen) and Enriched Germ Cell Transplantation into Common Carp (Cyprinus carpio) Testes

Successful Cryopreservation of Spermatogonia Stem Cells of Neotropical Catfish (Rhamdia quelen) and Enriched Germ Cell Transplantation into Common Carp (Cyprinus carpio) Testes

Biodiesel‐derived glycerol as a dietary maize replacement for silver catfish ( Rhamdia quelen ) during the fattening phase

Crude glycerin in feed for Colossoma macropomum fish: evaluation of histopathological effects on the liver and kidney

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