Silver and Copper Nanoparticles Inhibit Biofilm Formation by Mastitis Pathogens

Abstract

Bovine mastitis is a common bovine disease, frequently affecting whole herds of cattle. It is often caused by resistant microbes that can create a biofilm structure. The rapidly developing scientific discipline known as nanobiotechnology may help treat this illness, thanks to the extraordinary properties of nanoparticles. The aim of the study was to investigate the inhibition of biofilms created by mastitis pathogens after treatment with silver and copper nanoparticles, both individually and in combination. We defined the physicochemical properties and minimal inhibitory concentration of the nanoparticles and observed their interaction with the cell membrane, as well as the extent of biofilm reduction. The results show that the silver–copper complex was the most active of all nanomaterials tested (biofilm was reduced by nearly 100% at a concentration of 200 ppm for each microorganism species tested). However, silver nanoparticles were also effective individually (biofilm was also reduced by nearly 100% at a concentration of 200 ppm, but at concentrations of 50 and 100 ppm, the extent of reduction was lower than for the complex). Nanoparticles can be used in new alternative therapies to treat bovine mastitis.

Tailoring of Novel Azithromycin-Loaded Zinc Oxide Nanoparticles for Wound Healing

“…All of these findings show that raising the AZM concentration had a substantial impact on drug release. These findings are linked to the ease with which AZM can be Equations ( 13) and (14) show that the weight of ZnONP had a considerable favorable influence on drug release after 1 and 3 h in different formulations (9). At constant (X 1 ) and (X 3 ) (0, −1), the percentage of AZM released at the end of 3 h (Y 2 ) was 65.44 ± 2.71 and 75.23 ± 2.75% (D2, D13) at lower and higher ZnONPs weights, respectively.…”

“…Equations ( 13) and (14) show that the weight of ZnONP had a considerable favorable influence on drug release after 1 and 3 h in different formulations (9). At constant (X1) and (X3) (0, −1), the percentage of AZM released at the end of 3 h (Y2) was 65.44 ± 2.71 and 75.23 ± 2.75% (D2, D13) at lower and higher ZnONPs weights, respectively.…”

“…Nanomaterials have a great intrinsic potential for preventing and treating wound infections, with multiple advantages compared with traditional treatment approaches [3,4]. Metal and metal oxide nanoparticles [5][6][7] exhibit remarkable antibacterial activities against different pathogens, in addition to their low toxicity profile [8,9].…”

Antimicrobial Resistance of Salmonella enteritidis and Salmonella typhimurium Isolated from Laying Hens, Table Eggs, and Humans with Respect to Antimicrobial Activity of Biosynthesized Silver Nanoparticles

“…Furthermore, there are different factors that make AgNPs suitable for use as antimicrobial: (1) It is simple and safe to synthesize on a large scale with low cost [ 16 ]; (2) bacterial resistance to AgNPs is extremely rare; and (3) the surface of AgNPs can be easily modified and synthesized in various shapes [ 17 ]. Several studies have reported that AgNPs have antimicrobial activity against a variety of pathogens, including Salmonella enterica, E. coli O157:H7, Pseudomonas aeruginosa, Listeria monocytogenes, Klebsiella pneumoniae [ 18 ], and mastitis pathogens [ 19 ].…”

Antimicrobial second skin using copper nanomesh

“…It is well known that the wet condition of bacteria on the copper surface causes the cell membrane and cytoplasm to be damaged ( 40 ). In addition, the biofilm stretches until it ruptures in direct contact with the nanostructures of the copper nanomesh ( 41 ). After further exposure, the shapes of bacteria on the copper nanomesh became thin, irregular, wrinkled, and coarse, as shown in SI Appendix , Fig.…”